Expression of γ-tocopherol methyltransferase gene from Brassica napus increased α-tocopherol content in soybean seed

Chen, D. F.; Zhang, M.; Wang, Y. Q.; Chen, X. W.

doi:10.1007/s10535-011-0192-6

Cited by 8 publications

(12 citation statements)

References 18 publications

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“…The association that we observed between transcript abundance of Bo2g050970.1 in leaves and the c/atocopherol ratio in seeds is consistent with our understanding that tocopherols are synthesized and localized in plastids and accumulate in all tissues, with generally the highest content in seeds (Sattler et al, 2004). In Arabidopsis, c-TMT (VTE4, AT1G64970) is known to use dand c-tocopherols as substrates to produce b-and a-tocopherols, respectively (Shintani and DellaPenna, 1998), and the effect of the VTE4 gene from B. napus on a-tocopherol content has also been proven by overexpression in Glycine max (soya bean) and Arabidopsis (Endrigkeit et al, 2009;Chen et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Validation of an updated Associative Transcriptomics platform for the polyploid crop species Brassica napus by dissection of the genetic architecture of erucic acid and tocopherol isoform variation in seeds

Havlíčková

Wang

et al. 2017

The Plant Journal

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SummaryAn updated platform was developed to underpin association genetics studies in the polyploid crop species Brassica napus (oilseed rape). Based on 1.92 × 1012 bases of leaf mRNAseq data, functional genotypes, comprising 355 536 single‐nucleotide polymorphism markers and transcript abundance were scored across a genetic diversity panel of 383 accessions using a transcriptome reference comprising 116 098 ordered coding DNA sequence (CDS) gene models. The use of the platform for Associative Transcriptomics was first tested by analysing the genetic architecture of variation in seed erucic acid content, as high‐erucic rapeseed oil is highly valued for a variety of applications in industry. Known loci were identified, along with a previously undetected minor‐effect locus. The platform was then used to analyse variation for the relative proportions of tocopherol (vitamin E) forms in seeds, and the validity of the most significant markers was assessed using a take‐one‐out approach. Furthermore, the analysis implicated expression variation of the gene Bo2g050970.1, an orthologue of VTE4 (which encodes a γ‐tocopherol methyl transferase converting γ‐tocopherol into α‐tocopherol) associated with the observed trait variation. The establishment of the first full‐scale Associative Transcriptomics platform for B. napus enables rapid progress to be made towards an understanding of the genetic architecture of trait variation in this important species, and provides an exemplar for other crops.

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Section: Discussionmentioning

confidence: 99%

Validation of an updated Associative Transcriptomics platform for the polyploid crop species Brassica napus by dissection of the genetic architecture of erucic acid and tocopherol isoform variation in seeds

Havlíčková

Wang

et al. 2017

The Plant Journal

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“…) and Brassica napus (Chen et al. ). A cDNA encoding γ‐TMT from B. napus overexpressed in soybean seeds resulted in 11.1‐fold and 18.9‐fold increases in α‐Toc and β‐Toc contents, respectively (Chen et al.…”

mentioning

confidence: 99%

“…A cDNA encoding γ‐TMT from B. napus overexpressed in soybean seeds resulted in 11.1‐fold and 18.9‐fold increases in α‐Toc and β‐Toc contents, respectively (Chen et al. ). The γ‐TMT gene isolated from P. frutescens was overexpressed in soybean, resulting in 10.4‐fold (Tavva et al.…”

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confidence: 99%

Identification of the QTL underlying the vitamin E content of soybean seeds

Liu

Cao²,

Han

et al. 2017

Plant Breeding

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Vitamin E (VE) is an important antioxidant supplement for human health. Soybean seed extracts are the main source of VE supplements. Therefore, increasing the VE content of soybean seeds is important issue in breeding programmes. To detect quantitative trait loci (QTL) associated with VE in soybean seeds, 238 F6:7 recombinant inbred lines (RILs) were created by crossing a high VE cultivar, ‘Beifeng 9’, with a low VE cultivar, ‘Freeborn’. A genetic map was constructed using 218 polymorphic simple sequence repeat (SSR) markers. Composite interval mapping analysis detected 66 QTLs for contents of individual and total VE, 21 for α‐tocopherol, seventeen for γ‐tocopherol, thirteen for δ‐tocopherol and fifteen for total VE. The QTLs were located on chromosomes 9, 10, 15, 18 and 19. Phenotypic variance underlain by each QTL ranged from 2.4% to 32.6%. Two major QTLs (BARCSOYSSR_10_1140–BARCSOYSSR_10_1188 and BARCSOYSSR_15_0855 to BARCSOYSSR_15_0887) associated with α‐Toc, γ‐Toc, δ‐Toc and total VE contents were mapped on chromosomes 10 and 15. They explained 12.0% and 32.6% of phenotypic variance for α‐Toc; 5.5% and 13.0% for γ‐Toc; 6.6% and 23.6% for δ‐Toc; and 19.6% and 21.8% for total VE. QTL intervals BARCSOYSSR_15_0790–BARCSOYSSR_15_0855 (Qα15_1, Qγ15_1), BARCSOYSSR_15_1113–BARCSOYSSR_15_1159 (Qα15_3, Qδ15_2, QTVE15_4) and BARCSOYSSR_15_1159–BARCSOYSSR_15_1190 (Qα15_4, Qγ15_5, QTVE15_5) were associated with α‐Toc and explained 22.2%, 23.8% and 24.4% of the phenotypic variation in multiple environments. BARCSOYSSR_09_1098–BARCSOYSSR_09_1128 (QTVE9_1) and BARCSOYSSR_15_0887–BARCSOYSSR_15_0935 (QTVE15_2, Qγ15_3) associated with total VE content explained 21.8% and 16.4% of the phenotypic variation in two environments. These QTLs allow for marker‐assisted selection for cultivars with high VE contents.

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“…While g-tocopherol dominated the tocopherol composition of wild-type seeds (>95%), a-tocopherol represented up to 95% of seed tocopherols in the best transgenic event [23]. Subsequently, the successful conversion of g-tocopherol into a-tocopherol via g-TMT overexpression has been reported in many other plants including soybean [24][25][26][27], shiso [28], lettuce [29], mustard [30], maize [31], and tobacco [32]. Because of the higher biological activity of a-tocopherol, most g-TMT overexpressing crops exhibited 5-10 times higher vitamin E activity than untransformed plants.…”

Section: Update On the Tocochromanol Biosynthetic Pathwaymentioning

confidence: 98%

“…Because of the higher biological activity of a-tocopherol, most g-TMT overexpressing crops exhibited 5-10 times higher vitamin E activity than untransformed plants. In species accumulating d-tocopherol, which has 3% of the vitamin E activity of a-tocopherol, g-TMT overexpression also enhanced its conversion into b-tocopherol, which at 50% of the vitamin E activity of a-tocopherol is 16.6 times more potent [23][24][25][26][27]30] ( Figure 2 and Table 1). Because the gain in vitamin E activity is very significant and no adverse effects on growth and fertility have been reported in g-TMT overexpressing plants, this strategy is today among the most effective for vitamin E biofortification of crops.…”

Section: Update On the Tocochromanol Biosynthetic Pathwaymentioning

confidence: 99%

Current strategies for vitamin E biofortification of crops

Mène-Saffrané

Pellaud

2017

Current Opinion in Biotechnology

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Vitamin E refers to four tocopherols and four tocotrienols that are exclusively synthesized by photosynthetic organisms. While a-tocopherol is the most potent vitamin E compound, it is not the main form consumed since the composition of most major crops is dominated by g-tocopherol. Nutritional studies show that populations of developed countries do not consume enough vitamin E and that a large proportion of individuals exhibit plasma a-tocopherol deficiency. Following the identification of vitamin E biosynthetic genes, several strategies including metabolic engineering, classic breeding and mutation breeding, have been undertaken to improve the vitamin E content of crops. In addition to providing crops in which vitamin E content is enhanced, these studies are revealing the bottlenecks limiting its biosynthesis.

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Expression of γ-tocopherol methyltransferase gene from Brassica napus increased α-tocopherol content in soybean seed

Cited by 8 publications

References 18 publications

Validation of an updated Associative Transcriptomics platform for the polyploid crop species Brassica napus by dissection of the genetic architecture of erucic acid and tocopherol isoform variation in seeds

Validation of an updated Associative Transcriptomics platform for the polyploid crop species Brassica napus by dissection of the genetic architecture of erucic acid and tocopherol isoform variation in seeds

Identification of the QTL underlying the vitamin E content of soybean seeds

Current strategies for vitamin E biofortification of crops

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