Expression on human thymocytes of the idiotypic structures (Ti) from two leukemia T cell lines Jurkat and HPB‐ALL

Abstract

The expression on a significant number of thymocytes of idiotypic structures (Ti) restricted to HPB-ALL or Jurkat cells is demonstrated. As many as 2-4% of thymocytes were stained with anti-Ti HPB-ALL or anti-Ti Jurkat monoclonal antibodies, when analyzed by flow microfluorometry. Immunohistochemical localization studies performed on frozen thymus specimens of either fetal or pediatric origin indicated a scattered distribution of Ti-positive cells in both the cortex and the medulla. From lysates of 125I-labeled pediatric thymocytes, anti-Ti HPB-ALL and anti-Ti Jurkat monoclonal antibodies precipitated disulfide-linked heterodimers comparable to those precipitated from 125I-labeled HPB-ALL or Jurkat cells as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.

Biased t cell receptor v gene usage in rheumatoid arthritis. oligoclonal expansion of t cells expressing vα2 genes in synovial fluid but not in peripheral blood

“…Similar findings have been reported in 1 study using PCR techniques (17). However, direct comparison of our data with those obtained by PCR (13) (29). Consistent with our data, those authors found enrichment for Vp5.3-positive and/or Vp8-positive T cells in the synovial membrane of a subpopulation of patients.…”

Vβ5 andVβ8 memory T cells in adults and infancy: co-ordinated increase in response to early antigen stimulus

“…The K^s and K^j antibodies used here were originally raised by immunizing mice with the transformed T cell lines, HPB and Jurkat (Boylston & May, 1986;Carrel et al, 1986aCarrel et al, , 1986b, The frequencies we found of blood T cells bearing these determinants are similar to those previously reported in small studies of healthy adults. We extend previous observations by showing that the percentage of T cells expressing Vfi and K/ j g is independently variable in adults and in infants: this observation is consistent with the assumption that V region selection in cells developing in the thymus is random.…”

“…Additional aliquots were stained with FITC-conjugated UCHLl or CD45RA (Becton Dickinson) or FITC-CD3 and biotinylated antibody to K^j s (lCl) or Kjg (Mx6). IgG from ascites fluids of the lCl (Boylston & May, 1986) and Mx6 (Carrel et al, 1986b) hybridomas was prepared by elution from protein G (Pharmacia) at pH 2 8, neutralized and conjugated with A^-hydrosuccinimidobiotin (Sigma, no. H1759) for 4 h at pH 9, allowing 120 /ig per mg of protein.…”

T cell antigen receptor V gene usage. Increases in V beta 8+ T cells in Crohn's disease.

“…A growing number ofMAbs to human TCR V region products are being carefully characterized with respect to their specificity for various V gene products (4,5,9,11,12,18,19). These MAbs are useful tools to determine V gene usage by T cells in different tissue sites.…”