Expression pattern and association analysis of porcine matrix metallopeptidase 9 (MMP9) with diarrhea and performance traits in piglets

Kou, Mingxing; Guo, Dongchun; Liu, Lu; Gao, Xiaowen; Xing, Guiling; Zha, Andong; Shao, Bin; Sun, Yuan; Yang, Xiuqin; Wang, Xibiao; Di, Shengwei; Cai, Jiancheng; Niu, Buyue

doi:10.1016/j.rvsc.2019.12.017

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…Matrix metallopeptidase 9 (MMP9), a member of MMPs family, has been implicated in a variety of biological processes, such as inflammation and cell proliferation ( Kou et al, 2020 ; Zhang et al, 2020 ; Ringland et al, 2021 ). Mechanically, MMP9 can degrade many extracellular matrix (ECM) proteins and remodel the dynamic balance of ECM ( Huang, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Clostridium butyricum Protects Against Pancreatic and Intestinal Injury After Severe Acute Pancreatitis via Downregulation of MMP9

et al. 2022

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Background: Evidence have shown that gut microbiota plays an important role in the development of severe acute pancreatitis (SAP). In addition, matrix metalloproteinase-9 (MMP9) plays an important role in intestinal injury in SAP. Thus, we aimed to determine whether gut microbiota could regulate the intestinal injury during SAP via modulating MMP9.Methods: In this study, the fecal samples of patients with SAP (n = 72) and healthy controls (n = 32) were analyzed by 16S rRNA gene sequencing. In addition, to investigate the association between gut microbiota and MMP9 in intestinal injury during SAP, we established MMP9 stable knockdown Caco2 and HT29 cells in vitro and generated a MMP9 knockout (MMP9−/−) mouse model of SAP in vivo.Results: We found that the abundance of Clostridium butyricum (C. butyricum) was significantly decreased in the SAP group. In addition, overexpression of MMP9 notably downregulated the expressions of tight junction proteins and upregulated the expressions of p-p38 and p-ERK in Caco2 and HT29 cells (p < 0.05). However, C. butyricum or butyrate treatment remarkably upregulated the expressions of tight junction proteins and downregulated the expressions of MMP9, p-p38 and p-ERK in MMP9-overexpressed Caco2 and HT29 cells (p < 0.05). Importantly, C. butyricum or butyrate could not affect the expressions of tight junction proteins, and MMP9, p-p38 and p-ERK proteins in MMP9-knockdown cells compared with MMP9-knockdown group. Consistently, C. butyricum or butyrate could not attenuate pancreatic and intestinal injury during SAP in MMP9−/− mice compared with the SAP group.Conclusion: Collectively, C. butyricum could protect against pancreatic and intestinal injury after SAP via downregulation of MMP9 in vitro and in vivo.

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Section: Introductionmentioning

confidence: 99%

Clostridium butyricum Protects Against Pancreatic and Intestinal Injury After Severe Acute Pancreatitis via Downregulation of MMP9

et al. 2022

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“…Related studies found that MMP9 could play critical roles in many biological processes, such as reproduction, host defenses, cell proliferation and differentiation. MMP9 is a candidate gene for resistance to diarrhea (Kou et al., 2019). TNF is a target in TNF signaling pathway and is associated with inflammation (Chattopadhyay et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

“…The study demonstrates that suchilactone could tightly bind with the protein to relieve constipation (Fan et al., 2020), such as PIK3CA. The compound 20‐hexadecanoylingenol is positively associated with diarrhea controlling, especially MMP9 (Kou et al., 2019). The flavonoids vitexin 7‐glucoside weakly interacts with all the receptors, but it possesses an effect to relieve inflammation in mice (Peng et al., 2020).…”

Section: Discussionmentioning

confidence: 99%

Identification of the active compounds and their mechanisms of medicinal and edible Shanzha based on network pharmacology and molecular docking

Pei

Zheng

et al. 2021

Journal of Food Biochemistry

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Shanzha (Crataegus pinnatifida Bunge), an edible traditional Chinese medicine (TCM), has an effect on dyspepsia. However, the investigations of the pharmacological effects have not been carried out. This study aimed to identify the potential targets and pharmacological mechanisms of Shanzha in the treatment of dyspepsia by network pharmacology and molecular docking. Five active compounds and 13 key targets were obtained by a set of bioinformatics assays. Vitexin 7‐glucoside, suchilactone, and 20‐hexadecanoylingenol were the main compounds acting on dyspepsia. The key targets were prostaglandin‐endoperoxide synthase 2 (PTGS2), serine/threonine‐protein kinase mTOR (MTOR), heat shock protein HSP 90‐alpha (HSP90AA1), mitogen‐activated protein kinase 1 (MAPK1), MAPK3, E3 ubiquitin‐protein ligase Mdm2 (MDM2), receptor tyrosine‐protein kinase erbB‐2 (ERBB2), caspase‐3 (CASP3), matrix metalloproteinase‐9 (MMP9), estrogen receptor (ESR1), tumor necrosis factor (TNF), phosphatidylinositol 4,5‐bisphosphate 3‐kinase catalytic subunit alpha isoform (PIK3CA), and peroxisome proliferator‐activated receptor gamma (PPARG), which played the vital roles in TNF, prostate cancer, thyroid hormone, hepatitis B and estrogen signaling pathway. The molecular mechanisms of Shanzha regulating dyspepsia were mainly related to reduction of inflammatory response, controlling cell proliferation and survival, increasing intestinal moisture, and promoting intestinal motility. Practical applications Shanzha has been used as an edible TCM to improve digestion for a long time. However, the ingredients and mechanisms of Shanzha in the treatment of dyspepsia are not clear. In this research, network pharmacological analysis integrated with molecular docking was conducted to investigate the molecular mechanism. The results suggested that the core targets alleviated dyspepsia by reducing the intestinal inflammatory response, increasing intestinal movement, controlling cell physiological activities, and reducing constipation. In summary, this study demonstrated the multiple compounds, targets, and pathways characteristics of Shanzha in the treatment of dyspepsia, which may provide guidance and foundations for further application of edible medicine.

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Characterization, mRNA expression profile, subcellular distribution and association analysis with piglet diarrhea of porcine matrix metallopeptidase 7 (pMMP7)

Niu

Lü

Chen

et al. 2022

Gene

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Expression pattern and association analysis of porcine matrix metallopeptidase 9 (MMP9) with diarrhea and performance traits in piglets

Cited by 4 publications

References 24 publications

Clostridium butyricum Protects Against Pancreatic and Intestinal Injury After Severe Acute Pancreatitis via Downregulation of MMP9

Clostridium butyricum Protects Against Pancreatic and Intestinal Injury After Severe Acute Pancreatitis via Downregulation of MMP9

Identification of the active compounds and their mechanisms of medicinal and edible Shanzha based on network pharmacology and molecular docking

Characterization, mRNA expression profile, subcellular distribution and association analysis with piglet diarrhea of porcine matrix metallopeptidase 7 (pMMP7)

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