Expression pattern of a tobacco lipid transfer protein gene within the shoot apex

Fleming, Adam; Mandel, Therese; Hofmann, Sylvia; Sterk, Peter J.; Vries, SC de; Kuhlemeier, Cris

doi:10.1046/j.1365-313x.1992.t01-2-00999.x

Cited by 90 publications

(36 citation statements)

References 25 publications

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“…The conserved regions are boxed. C. Alignment of the deduced amino acid sequence of SDi-9 with other members of the LTP family, GLTPl from Gerbera hybrida [29], LPTl from Nicotiana tabacum [14], LTP from spinach 151 and PLTP from maize [40]. Both amino acid sequence identity and homology with SDi-9 protein are indicated in brackets at the end of each sequence.…”

Section: Discussionmentioning

confidence: 99%

Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype

et al. 1996

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We have studied two lines of sunflower (Helianthus annuus L.) selected in the field as drought-tolerant (R1 genotype) or drought-sensitive (S1 genotype). When subjected to drought conditions, the R1 line was able to maintain high leaf water potential longer and wilted later than the S1 line. Therefore, this indicates that R1 tolerance includes a leaf-adaptive response. By subtractive hybridization, we have isolated six different cDNAs (designated sdi for sunflower drought-induced) corresponding to transcripts accumulated in R1 and S1 leaves during adaptive response. Analysis of transcript accumulation in response to drought in both genotypes suggests a preferential expression of three sdi genes in the tolerant line. Abscisic acid-mediated induction, analysed in R1 leaves, was observed for only four sdi genes. Sequence analysis of six sdi clones revealed that five clones were related to known proteins including non specific lipid transfer proteins (nsLTP), early light-induced proteins (ELIP), l-aminocyclopropane-l-carboxylate oxidase (ACC oxidase) or dehydrins, predicted to be involved in a wide range of physiological processes.

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Section: Discussionmentioning

confidence: 99%

Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype

et al. 1996

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“…The volume of the hydrophobic cavity was calculated to be ca. [2,7,14,17,18,23]. On the basis of this result, it was suggested that a ns-LTP isoform might be expressed specifically in root tissue [1,2,17].…”

Section: Modelling Of Pvr3 Proteinmentioning

confidence: 90%

“…As shown in Fig. 5A The PVR3 amino acid sequence was aligned with LTP from Arabidopsis [22], barley [9], brassica [8], broccoli [14], carrot [18], caster bean [25], gerbera [11 ], maize [20], spinach [1 ], tobacco [7], tomato [24], Vigna [12], and wheat [5] using the program CLU STAL. The amino acid residues are presented in standard one letter code.…”

Section: Modelling Of Pvr3 Proteinmentioning

confidence: 99%

Isolation of a root-specific cDNA encoding a ns-LTP-like protein from the roots of bean (Phaseolus vulgaris L.) seedlings

Choi

Song

et al. 1996

Plant Mol Biol

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A root-specific cDNA clone, PVR3, was isolated from a bean (Phaseolus vulgaris L.) root cDNA library by a differential screening procedure. The nucleotide sequence of PVR3 contains an open reading frame coding for an 11.14 kDa polypeptide of 102 amino acid residues; the first 25 amino acids correspond to the sequence characteristic of a signal peptide. Comparison of the deduced PVR3 polypeptide sequence with the polypeptide sequences of previously cloned genes indicates that PVR3 may encode a ns-LTP-like protein. Molecular modelling of the PVR3 protein predicts that it has a three-dimensional structure that is similar to the three-dimensional model determined from the maize ns-LTP. The PVR3 mRNA accumulated mainly in the roots of young seedlings. It can be detected at low levels in flowers, but it is not detected in other organs. Genomic Southern blot analysis indicates that the genomic DNA corresponding to PVR3 cDNA is encoded by a single gene or small gene family in the bean genome.

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“…Nuclease S1 protection was described previously [10]. A uniformly 32p-labelled probe (nt + 143 to -188), prepared using the same primer as above, was hybridized to 50/2g total RNA in 80~o formamide at 37 °C, digested with 60 units nuclease S1 (Boehringer, Mannheim).…”

Section: Determination Of Transcription Start Sitementioning

confidence: 99%

“…2,5,10,24,30]. However, there are relatively few reports on the expression of constitutively expressed genes.…”

Section: Introductionmentioning

confidence: 99%

Definition of constitutive gene expression in plants: the translation initiation factor 4A gene as a model

et al. 1995

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The NelF-4A 10 gene belongs to a family of at least ten genes, all of which encode closely related isoforms of translation initiation factor 4A. The promoter region of NelF-4A10 was sequenced, and four m R N A 5' ends were determined. Deletions containing 2750, 689 and 188 bp of untranscribed upstream DNA were fused to the GUS reporter gene and introduced into transgenic tobacco. The three constructs mediated GUS expression in all cells of the leaf, stem and shoot apical meristem. Control experiments using in situ hybridization and tissue printing indicated that the observed GUS expression matches the expression patterns of NelF-4A m R N A and protein. This detailed analysis at the level ofmRNA, protein and reporter gene expression shows that NelF-4A10 is an ideal constitutively expressed control gene. We argue that inclusion of such a control gene in experiments dealing with specifically expressed genes is in many cases essential for the correct interpretation of observed expression patterns.

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Expression pattern of a tobacco lipid transfer protein gene within the shoot apex

Cited by 90 publications

References 25 publications

Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype

Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype

Isolation of a root-specific cDNA encoding a ns-LTP-like protein from the roots of bean (Phaseolus vulgaris L.) seedlings

Definition of constitutive gene expression in plants: the translation initiation factor 4A gene as a model

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