Expression pattern of acidic and basic fibroblast growth factor genes in adult rat eyes

Noji, Sumihare; Matsuo, Toshihiko; Koyama, Eiki; Yamaai, Tomoichiro; Nohno, Tsutomu; Matsuo, Nobuhiko; Taniguchi, Shigehiko

doi:10.1016/0006-291x(90)91714-4

Cited by 83 publications

(37 citation statements)

References 9 publications

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“…The coincident expression of aFGF during initial lens differentiation with that of a, p, and y-crystallins and within regions of high mitotic activity (McAvoy, 1978) may indicate that an autocrine mechanism is involved in regulating lens cell behaviour. This is supported by the finding that embryonic mouse (Wanaka et al, 1991) and rat lens cells express FGF receptor mRNA and that aFGF mRNA has been localised in postnatal rat lens by in situ hybridisation (Noji et al, 1990). In addition, the untruncated form of aFGF has been isolated from adult bovine lens cells (Schulz et al, 1993).…”

Section: Discussionmentioning

confidence: 90%

Spatio‐temporal distribution of acidic and basic FGF indicates a role for FGF in rat lens morphogenesis

Iongh

McAvoy

1993

Developmental Dynamics

111

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As part of an investigation into the role FGF in lens development, we have studied the distribution of both aFGF and bFGF during eye morphogenesis from embryonic days 10 to 18 (E10–E18) in the rat. For aFGF, reactivity was found only in ectoderm at E10, prior to contact between the optic vesicle and presumptive lens ectoderm. During lens placode formation (E11) there was a transient, diffuse reactivity for aFGF in anterior optic vesicle cells directly apposed to the labelled ectoderm of the lens placode. At E12 the diffuse reactivity of the lens placode had changed to a discrete localisation along the basolateral surfaces of differentiating cells in the lens pit. Similar reactivity was associated with neuroblasts along the inner margin of the optic cup. At the early lens vesicle stage (E13) the baso‐lateral aFGF‐like reactivity associated with elongating lens cells was more intense and extensive. From the late lens vesicle stage (E14) to E18, reactivity in the lens was increasingly restricted to the equatorial regions which incorporate the germinative and transitional zones. From E16 to E18, aFGF‐like reactivity in the retina was predominantly localised in the peripheral regions corresponding to the developing ciliary body and iris and in the central retina associated with ganglion cell axons. For bFGF, weak reactivity was detectable as early as E13 in the developing lens capsule and increased in intensity during lens development with the posterior capsule reacting more intensely than the anterior capsule. Retinal bFGF‐like reactivity was first detected at E14, associated with differentiating ganglion cells in the central retina. From E16 to E18 the retinal ganglion cells showed increasing reactivity and the pattern of reactivity followed the centro‐peripheral pattern of retinal development. Thus reactivity for aFGF is first detected in presumptive lens ectoderm and subsequently in optic vesicle cells which are closely associated with lens ectoderm. This raises the possibility that aFGF may be involved in inductive interactions between presumptive lens ectoderm and optic vesicle. Furthermore the localisation patterns established for both aFGF and bFGF during lens and retina morphogenesis suggest an important role for FGF in regulating their morphogenesis and growth. © 1993 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 90%

Spatio‐temporal distribution of acidic and basic FGF indicates a role for FGF in rat lens morphogenesis

Iongh

McAvoy

1993

Developmental Dynamics

111

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“…1 G-I) raises the distinct possibility that this cell type may synthesize and release bFGF into the IPM; but neither the photoreceptors nor the RPE can be excluded from such a role. RPE cells have the capacity to synthesize bFGF under in vitro conditions (45), and recent in situ hybridization studies using 35S-labeled antisense probes to detect bFGF mRNA have found evidence of photoreceptor inner segment labeling in the rat (46). Moreover, 125I-labeled aFGF and bFGF bind to isolated preparations of bovine rod outer segments and disc membranes (27,29).…”

Section: Discussionmentioning

confidence: 99%

Sequestration of basic fibroblast growth factor in the primate retinal interphotoreceptor matrix.

Hageman¹,

Kirchoff-Rempe²,

Lewis³

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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The interphotoreceptor matrix (IPM) occupies the extracellular space between the photoreceptors of the retina and the apical surface of the retinal pigmented epithelium. A large proportion of the IPM is composed of aqueousinsoluble glycoconjugates, including chondroitin sulfatecontaining proteoglycans, the distribution of which exhibits both apical-basal and photoreceptor cell type-specific heterogeneities. The precise function of most insoluble IPM constituents is unknown, although the available evidence suggests some may contribute to retinal adhesion or photoreceptor survival. We have now identified basic fibroblast growth factor (bFGF), or an immunologically related protein from the FGF family, within the IPM. The IPM is labeled on sections of primate retinas by a battery of polyclonal antibodies (Abs) directed against various peptide sequences of bFGF and by an Ab to bovine brain bFGF. bFGF Abs also bind to purified preparations of aqueous-insoluble IPM. AU bFGF Abs utilized cross-react with equivalent low molecular mass components of 16.5-17.5 kDa on Western blots of insoluble IPM proteins, purified bFGF, and recombinant bFGF. The Abs do not bind any aqueous-soluble IPM components, suggesting that the bFGF is normally bound to an insoluble 1PM constituent(s) in situ. The fact that bFGF is sequestered in the IPM and is located in such close proximity to the photoreceptors, the retinal pigmented epithelium, and Mueller's glia raises the strong possibility that it is synthesized by and regulates the activities of one or more of these three cell types in vivo.The fibroblast growth factors (FGFs) are a family of peptides that have been isolated from a broad range of cell types. FGFs share a number of structural, biochemical, and biological properties, the most characteristic being that they bind to heparin (1-4). The FGF family consists of acidic FGF (aFGF), basic FGF (bFGF), and a number of other related molecules that exhibit significant sequence homology to these two proteins. FGFs, like some other growth factors, are regarded as multifunctional in that they have a range of effects and activities in vitro that depend upon the target cell type (5). To date, FGFs have been shown: to influence neuronal differentiation, survival, and regeneration (6-9); to act as mitogens (10-13) and as inducing factors (14-17); to modulate protein synthesis (12,18); and to induce cell motility and migration (19). Much more is known about the effects of FGFs in vitro than under in vivo conditions.Although biochemical assays have shown that FGFs are present in a wide variety of cell types and tissues (for review, see refs. 3 and 4), only a few studies have sought to determine their specific cellular and/or extracellular distributions. Antibodies (Abs) have been used to localize bFGF to the cytoplasm of rat brain neurons (20-22) and chicken striated muscle myoblasts (23) and to the extracellular matrix of mouse hind-limb muscle (24). A recent comprehensive study of bFGF distribution in the 18-day rat fetus revealed intrace...

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“…The neural retina of stage 22-24 chick embryos does not normalfy regenerate after retinectomy, but if contact between the outer kyer of the optic cup (which forms the RPE) and inner kyer (which forms the NR kyers) is prevented, the RPE differentktes as NR (Orts-Llorca and GenisGalvez, 1960). Both the RPE and NR of adult rat eyes express FGFs and their receptors (Noji et al, 1990). Regeneration of the NR from the RPE can be induced by impknting a piece of NR into the vitreous chamber (Coulombre and Coulombre, 1970).…”

Section: Dediffekntiation/tmnsde Terminationmentioning

confidence: 99%

Rx for tissue restoration: Regenerative biology and medicine

Stocum¹

2001

Korean Journal of Biological Sciences

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Expression pattern of acidic and basic fibroblast growth factor genes in adult rat eyes

Cited by 83 publications

References 9 publications

Spatio‐temporal distribution of acidic and basic FGF indicates a role for FGF in rat lens morphogenesis

Spatio‐temporal distribution of acidic and basic FGF indicates a role for FGF in rat lens morphogenesis

Sequestration of basic fibroblast growth factor in the primate retinal interphotoreceptor matrix.

Rx for tissue restoration: Regenerative biology and medicine

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