Expression pattern of Boule in dairy goat testis and its function in promoting the meiosis in male germline stem cells (mGSCs)

Li, Mingzhao; Liu, Chao; Zhu, Haijing; Sun, Junwei; Yu, Meng; Niu, Zhiwei; Liu, Weishuai; Peng, Sha; Hua, Jinlian

doi:10.1002/jcb.24368

Cited by 28 publications

(38 citation statements)

References 41 publications

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“…Dazl and Boule are two members of the Daz family which encode proteins required for meiosis [Kim et al, 2012]. Disruption of Dazl or Boule leads to meiotic arrest, spermatogenetic failure and eventually male infertility [Ruggiu et al, 1997;Jenkins et al, 2011;Liu et al, 2011;Li et al, 2012]. We revealed that Dazl and Boule had a significantly lower expression in the SU5416-treated group compared to the control or VEGF-treated grafts.…”

Section: Discussionmentioning

confidence: 77%

VEGF/VEGFR2 Signaling Regulates Germ Cell Proliferation in vitro and Promotes Mouse Testicular Regeneration in vivo

Tian

Yang

Zhu

et al. 2016

Cells Tissues Organs

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Vascular endothelial growth factor (VEGF) plays fundamental roles in testicular development; however, its function on testicular regeneration remains unknown. The objective of this study was to explore the roles VEGF/VEGFR₂ signaling plays in mouse germ cells and in mouse testicular regeneration. VEGF and the VEGFR₂ antagonist SU5416 were added to culture medium to evaluate their effects on spermatogonial stem cell line (C18-4 cells) proliferation. Testicular cells obtained from newborn male ICR mice were grafted into the dorsal region of male BALB/c nude mice. VEGF and SU5416 were injected into the graft sites to assess the effects of the VEGF and VEGFR₂ signaling pathways on testicular reconstitution. The grafts were analyzed after 8 weeks. We found that VEGF promoted C18-4 proliferation in vitro, indicating its role in germ cell survival. HE staining revealed that seminiferous tubules were reconstituted and male germ cells from spermatogonia to spermatids could be observed in testis-like tissues 8 weeks after grafting. A few advantaged male germ cells, including spermatocytes and spermatids, were found in SU5416-treated grafts. Moreover, VEGF enhanced the expression of genes specific for male germ cells and vascularization in 8-week grafts, whereas SU5416 decreased the expression of these genes. SU5416-treated grafts had a lower expression of MVH and CD31, indicating that blockade of VEGF/VEGFR₂ signaling reduces the efficiency of seminiferous tubule reconstitution. Collectively, these data suggest that VEGF/VEGFR₂ signaling regulates germ cell proliferation and promotes testicular regeneration via direct action on germ cells and the enhancement of vascularization.

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Section: Discussionmentioning

confidence: 77%

VEGF/VEGFR2 Signaling Regulates Germ Cell Proliferation in vitro and Promotes Mouse Testicular Regeneration in vivo

Tian

Yang

Zhu

et al. 2016

Cells Tissues Organs

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“…For the FACS-mediated cell purity assay, the cells were digested by trypsin and washed in PBS at room temperature, cells without GFP were used as negative control and set 0.1% as gating. FACS was performed on an EPICS ELITE apparatus (Beckmann-Coulter) with MultiCycle software (Phoenix Flow Systems, Inc.)63.…”

Section: Methodsmentioning

confidence: 99%

The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

Zheng

Zhai

et al. 2016

Sci Rep

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Epigenetic modification plays key roles in spermatogenesis, especially DNA methylation dynamic is important in sustaining normal spermatogenesis. Ten-eleven translocation 1 (Tet1) is not only a key demethylase, which works in specific gene regions, but also crosstalks with partners to regulate epigenetic progress as protein complexes. Dairy goat is an important livestock in China, while the unstable culture system in vitro inhibits optimization of new dairy goat species. The study of epigenetic modification in male germline stem cells (mGSCs) is beneficial to the optimization of adult stem cell culture system in vitro, and the improvement of sperm quality and breeding of selected livestock. In our study, we not only analyzed the morphology, gene expression, DNA methylation and histone methylation dynamic in mouse Tet1 (mTet1) modified mGSCs, we also analyzed the stemness ability by in vivo transplantation and explored the functional mechanism of Tet1 in dairy goat mGSCs. The results showed mTet1 modified mGSCs had better self-renewal and proliferation ability than wild-type mGSCs, mTet1 could also up-regulate JMJD3 to decrease H3K27me3, which also showed to suppress the MEK-ERK pathway. Furthermore, Co-IP analysis demonstrated that TET1 interact with PCNA and HDAC1 by forming protein complexes to comprehensively regulate dairy goat mGSCs and spermatogenesis.

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“…Sections were then washed in 1 × PBS three times and incubated with a goat anti-rabbit IgG antibody conjugated to Alexa Fluor 488 (Invitrogen) for 1 h at RT. Coverslips were mounted onto slides with Anti-fading Buffer mounting medium (Bioworld) containing 1 μg/ml Hoechst33342 (Sigma)4951.…”

Section: Methodsmentioning

confidence: 99%

Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT

Zhou

et al. 2016

Sci Rep

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Lin28a is a conserved RNA-binding protein that plays an important role in development, pluripotency, stemness maintenance, proliferation and self-renewal. Early studies showed that Lin28a serves as a marker of spermatogonial stem cells (SSCs) and promotes the proliferation capacity of mouse SSCs. However, there is little information about Lin28a in livestock SSCs. In this study, we cloned Capra hircus Lin28a CDS and found that it is evolutionarily conserved. Lin28a is widely expressed in different tissues of Capra hircus, but is expressed at a high level in the testis. Lin28a is specifically located in the cytoplasm of Capra hircus spermatogonial stem cells and may also be a marker of dairy goat spermatogonial stem cells. Lin28a promoted proliferation and maintained the self-renewal of GmGSCs-I-SB in vivo and in vitro. Lin28a-overexpressing GmGSCs-I-SB showed an enhanced proliferation rate, which might be due to increased PCNA expression. Moreover, Lin28a maintained the self-renewal of GmGSCs-I-SB by up-regulating the expression of OCT4, SOX2, GFRA1, PLZF and ETV5. Furthermore, we found that Lin28a may activate the AKT, ERK, and mTOR signaling pathways to promote the proliferation and maintain the self-renewal of GmGSCs-I-SB.

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Expression pattern of Boule in dairy goat testis and its function in promoting the meiosis in male germline stem cells (mGSCs)

Cited by 28 publications

References 41 publications

VEGF/VEGFR<sub>2</sub> Signaling Regulates Germ Cell Proliferation in vitro<b> </b>and Promotes Mouse Testicular Regeneration in vivo

VEGF/VEGFR<sub>2</sub> Signaling Regulates Germ Cell Proliferation in vitro<b> </b>and Promotes Mouse Testicular Regeneration in vivo

The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT

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