Expression pattern of cellulolytic and xylanolytic genes regulated by transcriptional factors XYR1 and CRE1 are affected by carbon source in Trichoderma reesei

Castro, Lilian dos Santos; Antoniêto, Amanda Cristina Campos; Pedersoli, Wellington Ramos; Silva‐Rocha, Rafael; Persinoti, Gabriela Félix; Silva, Roberto Nascimento

doi:10.1016/j.gep.2014.01.003

Cited by 54 publications

(73 citation statements)

References 29 publications

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“…These ions participate in essential processes, such as electron transport through the respiratory chain to produce ATP (Dmitriev et al, 2013), suggesting that the metabolism of the fungus decelerates when CRE1 is active. Copper and iron can also act as final electron acceptors for the enzyme cellobiose dehydrogenase (enzyme which in T. reesei has possible candidates to its function), which oxidizes several sugars, such as cellobiose, glucose oligomers, and cellulose (Dos Santos Castro et al, 2014). The reduction of Fe 3+ to Fe 2+ can generate hydroxyl radicals through the Fenton reaction, promoting the depolymerization of polysaccharides and structural modification of cell wall lignins (Henriksson et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, this is the first time that such a mechanism, in which multilevel control of cellulase expression and activity is regulated by CRE1, has been described in filamentous fungi. However, because the filamentous fungi that degrade lignocellulose polymers can respond differently to inducers (Dos Santos Castro et al, 2014;Znameroski et al, 2012), additional comparative studies are necessary to understand the CCR process.…”

Section: Discussionmentioning

confidence: 99%

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Defining the genome-wide role of CRE1 during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis

Antoniêto

Castro

Silva‐Rocha

et al. 2014

Fungal Genetics and Biology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Defining the genome-wide role of CRE1 during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis

Antoniêto

Castro

Silva‐Rocha

et al. 2014

Fungal Genetics and Biology

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“…Since glucose was used as a carbon source in the present study, one can expect that in a natural biotope (glucose poor), more CAZY could be expressed. Indeed, it has been shown that cellulolytic and xylanolytic genes are regulated by transcriptional factors XYR1 and CRE1 in Trichoderma reesei depending on the carbon source, with glucose acting as a repressor (39).…”

Section: Transcriptomic Analysis (I) Overview Of Gene Expressionmentioning

confidence: 99%

Transcriptomic Responses of Phanerochaete chrysosporium to Oak Acetonic Extracts: Focus on a New Glutathione Transferase

Thuillier

Chibani

Bellı́

et al. 2014

Appl Environ Microbiol

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The first steps of wood degradation by fungi lead to the release of toxic compounds known as extractives. To better understand how lignolytic fungi cope with the toxicity of these molecules, a transcriptomic analysis of Phanerochaete chrysosporium genes was performed in the presence of oak acetonic extracts. It reveals that in complement to the extracellular machinery of degradation, intracellular antioxidant and detoxification systems contribute to the lignolytic capabilities of fungi, presumably by preventing cellular damages and maintaining fungal health. Focusing on these systems, a glutathione transferase (P. chrysosporium GTT2.1 [PcGTT2.1]) has been selected for functional characterization. This enzyme, not characterized so far in basidiomycetes, has been classified first as a GTT2 compared to the Saccharomyces cerevisiae isoform. However, a deeper analysis shows that the GTT2.1 isoform has evolved functionally to reduce lipid peroxidation by recognizing high-molecular-weight peroxides as substrates. Moreover, the GTT2.1 gene has been lost in some non-wood-decay fungi. This example suggests that the intracellular detoxification system evolved concomitantly with the extracellular ligninolytic machinery in relation to the capacity of fungi to degrade wood.

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“…Genes encoding GH-5, GH-6, GH-7 and GH-61 (AA9) were found to be differentially expressed by P. chrysosporium cultured on cellulose (GSE14734), ball milled aspen (GSE14734, GSE27941). It was reported that, in Trichoderma reesei genes coding for cellulolytic and xylanolytic enzymes are regulated by XYR1 and CRE1 transcriptional factors corresponding to the carbon source used for its growth 43. It was also seen that, presence of glucose in the growth media represses the expression of cellulolytic and xylanolytic genes 44-47.…”

Section: Resultsmentioning

confidence: 99%

Metadata Analysis of Phanerochaete chrysosporium Gene Expression Data Identified Common CAZymes Encoding Gene Expression Profiles Involved in Cellulose and Hemicellulose Degradation

Kameshwar

Qin

2017

Int. J. Biol. Sci.

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In literature, extensive studies have been conducted on popular wood degrading white rot fungus, Phanerochaete chrysosporium about its lignin degrading mechanisms compared to the cellulose and hemicellulose degrading abilities. This study delineates cellulose and hemicellulose degrading mechanisms through large scale metadata analysis of P. chrysosporium gene expression data (retrieved from NCBI GEO) to understand the common expression patterns of differentially expressed genes when cultured on different growth substrates. Genes encoding glycoside hydrolase classes commonly expressed during breakdown of cellulose such as GH-5,6,7,9,44,45,48 and hemicellulose are GH-2,8,10,11,26,30,43,47 were found to be highly expressed among varied growth conditions including simple customized and complex natural plant biomass growth mediums. Genes encoding carbohydrate esterase class enzymes CE (1,4,8,9,15,16) polysaccharide lyase class enzymes PL-8 and PL-14, and glycosyl transferases classes GT (1,2,4,8,15,20,35,39,48) were differentially expressed in natural plant biomass growth mediums. Based on these results, P. chrysosporium, on natural plant biomass substrates was found to express lignin and hemicellulose degrading enzymes more than cellulolytic enzymes except GH-61 (LPMO) class enzymes, in early stages. It was observed that the fate of P. chrysosporium transcriptome is significantly affected by the wood substrate provided. We believe, the gene expression findings in this study plays crucial role in developing genetically efficient microbe with effective cellulose and hemicellulose degradation abilities.

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Expression pattern of cellulolytic and xylanolytic genes regulated by transcriptional factors XYR1 and CRE1 are affected by carbon source in Trichoderma reesei

Cited by 54 publications

References 29 publications

Defining the genome-wide role of CRE1 during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis

Defining the genome-wide role of CRE1 during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis

Transcriptomic Responses of Phanerochaete chrysosporium to Oak Acetonic Extracts: Focus on a New Glutathione Transferase

Metadata Analysis of Phanerochaete chrysosporium Gene Expression Data Identified Common CAZymes Encoding Gene Expression Profiles Involved in Cellulose and Hemicellulose Degradation

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