Expression pattern of NLRC5 in the postnatal mouse brain

Hu, Yue; Shen, Yingqiang; Wu, Xiaojing; Ba, Ru; Xu, Hui; Lu, Keze; Shao, Yong; Sun, Chen; Zhang, Ying; Miao, Fengqin; Shen, Yuqing; Zhang, Jianqiong

doi:10.1016/j.acthis.2022.151939

Cited by 1 publication

(1 citation statement)

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“…Until recently, the expression and function of NLRC5 in the central nervous system have not been fully clarified. NLRC5 is found to be expressed in neurons and microglia but rarely in astrocytes in developing and adult mouse brains [14,15]. NLRC5 is also expressed by BV2 cells, a classical microglial cell line [14].…”

Section: Introductionmentioning

confidence: 99%

NLRC5 Deficiency Reduces LPS-Induced Microglial Activation via Inhibition of NF-κB Signaling and Ameliorates Mice’s Depressive-like Behavior

Sun,

Shen,

Liu

et al. 2023

IJMS

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Microglia are believed to be the key immune effectors of the central immune microenvironment, and their dysregulation is associated with neuroinflammation and mood disorders. Nucleotide-binding oligomerization domain-like receptor family caspase recruitment domain-containing five (NLRC5) is a new member of the Nod-like receptor family. Recently, NLRC5 has been reported to be expressed by microglia. Nonetheless, the exact roles of NLRC5 in microglial activation and its function in depression have not been investigated yet. Herein, we found that reducing NLRC5 decreased lipopolysaccharide (LPS)-induced secretion of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) in primary cultured microglia and microglial cell lines but not in bone marrow-derived macrophages (BMDMs). In more detail, reducing NLRC5 diminished the secretion of LPS-induced cytokines by attenuating IKKα/β phosphorylation and inhibiting NF-κB signaling. Moreover, the expression of Nlrc5 in the hippocampus of LPS- or chronic unpredictable mild stress (CUMS)-induced depressive mice was increased. In line with the in vitro findings, Nlrc5 deficiency inhibited microglial activation in the mouse hippocampus and improved LPS- or CUMS-induced depressive-like behaviors. In summary, we demonstrated the critical role of NLRC5 in LPS-induced microglial activation and LPS- or CUMS-induced depressive mouse models.

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Section: Introductionmentioning

confidence: 99%