Expression pattern of uricase II gene during root nodule development inPhaseolus vulgaris

Papadopoulou, Kalliopi; Roussis, Andreas; Kuin, H.M.A.; Katinakis, Panagiotis

doi:10.1007/bf01964927

Cited by 16 publications

(16 citation statements)

References 27 publications

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“…We also performed in situ hybridization experiments on 21-d-old nodule sections as a positive control. We detected the uricase II mRNA not only in the uninfected cells of the central tissue, as previously reported (Tate et al, 1994;Papadopoulou et al, 1995), but also in the nodule parenchyma cells and in the vascular tissue (Fig. 9G).…”

Section: Spatial Localization Of Uricase II Transcripts In the Roots supporting

confidence: 88%

“…Uricase I1 transcripts have been localized by. in situ hybridization in the uninfected cells of the central tissue of bean nodules (Taté et al, 1994;Papadopoulou et al, 1995). However, we have also detected uricase mRNA in the vascular tissue that traverses the nodule as well as in nodule parenchyma cells (Fig.…”

Section: Discussionmentioning

confidence: 60%

“…Vol. 1 1 5, 1997 jackbean (Canavalia liniata) (Kim and An, 1993), and bean (Sánchez et al, 1987;Papadopoulou et al, 1995) nodule libraries.…”

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confidence: 99%

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Characterization of the Common Bean Uricase II and Its Expression in Organs Other than Nodules

Capote

Sánchez

1997

Plant Physiology

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Uricase II is a purine metabolic enzyme highly induced in root nodules during the symbiosis established between legumes and bacteria of the genera Rhizobium and Bradyrhizobium. Here we describe the characterization of bean (Phaseolus vulgaris) nodule uricase II cDNA and show that uricase I I is encoded by a single gene in the bean genome. This gene is also expressed in cotyledons, roots, and hypocotyls during bean seedling establishment, and an antiuricase antibody recognizes the protein in different seedling organs. Uricase II has also been found in leucaena leucocephala seedlings, suggesting that it participates during seedling establishment in legumes that do not transport ureides. A 50-kD polypeptide that is detected by the anti-uricase antibody is found in cotyledons during seedling development. This higher-molecular-mass form is also detected in developing roots and hypocotyls but not in nodules. In situ hybridization experiments in root seedlings showed uricase II transcripts in the metaxylem parenchyma cells and phloem fibers of the vascular system.

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Section: Spatial Localization Of Uricase II Transcripts In the Roots supporting

confidence: 88%

Section: Discussionmentioning

confidence: 60%

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Characterization of the Common Bean Uricase II and Its Expression in Organs Other than Nodules

Capote

Sánchez

1997

Plant Physiology

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“…Nothing is known about expression regulation of these uricases. Synthesis of nodulin-35 takes place during the nodule ontogeny in uninfected cells of Glycine max and Phaseolus vulgaris (Bergmann et al 1983, Nguyen et al 1985, Sfinchez et al 1987, Papadopoulou et al 1995, and the enzyme synthesis is regulated by oxygen at the mRNA level in Glycine max callus tissue Jochimsen 1986, Xue et al 1991). However, little is known about the regulation of uricase synthesis in the green alga Chlamydomonas reinhardtii.…”

Section: Introductionmentioning

confidence: 96%

Urate-mediated regulation of urate oxidase inChlamydomonas reinhardtii

et al. 1998

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Summary.Expression of uricase (urate oxidase) from Chlamydomonas reinhardtii has been investigated by using specific polyclonal antibodies. By Western blot analyses performed under nondenaturing conditions, a 124 kDa protein band corresponding to active uricase was detected in protein extracts from cells cultured with urate or nitrogen-starved cells. This protein band was absent in cells cultured with ammonium. Besides the 124 kDa band, the antibodies also reacted with a massive protein band, with an apparent molecular mass of 500 kDa, that was detected in all nutritional conditions assayed. In vitro, inactive uricase from cells grown with ammonium was activated by incubation in presence of urate. The appearance of uricase activity in vitro coincided with a decrease of the 500 kDa protein and an increase of the 124 kDa band corresponding to the active enzyme. We suggest that a posttranslational regulation of uricase synthesis takes place in C. reinhardtii, and that urate may be responsible for the assembly or maturation of inactive precursors to form the active uricase.

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“…Its homologues were also present in same non-leguminous plants -tobacco and rice Genomes of different legumes contain more than one copy of that gene (for example soybean, alfalfa and French bean) (Kouchi and Hata 1993, Papadopoulou et al 1996, Fang and Hirch 1998. All these E N O D 4 0 genes contain two highly conserved short open reading frames (sORF); called boxes I and II (Fig.…”

Section: Enod40mentioning

confidence: 98%

Plant signalling peptides

2003

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A b s t r a c tBiochemical and genetic studies have identified peptides that play crucial roles in plant growth and development, including defence mechanisms in response to wounding by pests, the control of cell division and expansion, and pollen self-incompatibility. The first two signalling peptides to be described in plants were tomato systemin and phytosulfokine (PSK). There is also biochemical evidence that natriuretic peptide-like molecules, immunologically-related to those found in animals, may exist in plants. Another example of signalling peptide is ENOD40, a product of a gene, which became active early in the root nodulation process following Rhizobium infection of legumes. Other predicted bioactive peptides or oligopeptides have been identified by means of genetic, rather then biochemical methods. The Arabidopsis CLAVATA3 protein is required for the correct organization of the shoot apical meristem and the pollen S determinant S-locus cysteine-rich protein (SCR also called S-locus protein 11, SP11).The plant signalling peptides discovered so far are involved in various processes and play an important role in communication between cells or organs, respectively. This review will focus on these peptides and their role in intercellular signalling.

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Expression pattern of uricase II gene during root nodule development inPhaseolus vulgaris

Cited by 16 publications

References 27 publications

Characterization of the Common Bean Uricase II and Its Expression in Organs Other than Nodules

Characterization of the Common Bean Uricase II and Its Expression in Organs Other than Nodules

Urate-mediated regulation of urate oxidase inChlamydomonas reinhardtii

Plant signalling peptides

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