Expression Patterns, Activities and Sugar Metabolism Regulation of Sucrose Phosphate Synthase, Sucrose Synthase, Neutral Invertase and Soluble Acid Invertase in Different Goji Cultivars during Fruit Development

Wang, T.; Wright, Donald N.; Xu, Haoyang; Yang, Yaqin; Rui, Zheng; Shi, Jinhua; Chen, R.; Wang, L.

doi:10.1134/s1021443719010199

Cited by 16 publications

(13 citation statements)

References 24 publications

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“…In addition, the differential expression patterns among roots, stems, leaves, and fruits under different CO 2 conditions revealed that these genes have diverse spatiotemporal expression patterns. A study found that SPS and NI expression levels correlated with the corresponding enzyme activities during goji berry fruit development, whereas those of AI and SS were not related to changes in enzyme activities in different cultivars [24]. Our data further demonstrates that these four sucrose metabolism-related genes play an important role in sugar metabolism and respond positively to different CO 2 concentrations in goji berry plants.…”

Section: Regulation Of Sucrose Metabolism-related Gene Expressionsupporting

confidence: 59%

“…This study identi ed and isolated four sucrose metabolism-related genes-LBAI, LBSS, LBSPS, and LBNIbased on the de novo transcriptome of goji berry [27]; BLAST searches indicated that the sequences of these genes had high homology with the reported sequences for goji berry [24]. In addition, the differential expression patterns among roots, stems, leaves, and fruits under different CO 2 conditions revealed that these genes have diverse spatiotemporal expression patterns.…”

Section: Regulation Of Sucrose Metabolism-related Gene Expressionmentioning

confidence: 96%

“…Numerous studies have explained how different fruit sugar levels in different cultivars are maintained [24]. Additionally, physiological studies have shown a close correlation between sucrose metabolismrelated enzymes and different kinds of sugars in goji berries [25].…”

Section: Introductionmentioning

confidence: 99%

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WITHDRAWN: Effect of Elevated CO2 on Photosynthetic Accumulation and Sucrose Metabolism-related Enzyme Activity and Gene Expression Patterns in Goji Berry (Lycium Barbarum L.)

Xie

et al. 2020

Preprint

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Background: Exposing goji berries to elevated CO2 (eCO2) for long periods reduces sugar and secondary metabolite contents. However, sugar accumulation in fruit depends on photosynthesis and photoassimilate partitioning. The objectives of this study were a) to explore photosynthesis, sugar content, and sucrose metabolism-related enzyme activity in goji berry leaves and fruits under ambient and elevated CO2, and b) to identify the genes encoding Lycium barbarum acid invertase (LBAI), L. barbarum sucrose synthase (LBSS), L. barbarum sucrose phosphate synthase (LBSPS), and L. barbarum neutral invertase (LBNI) based on transcriptome profiling and expression analyses in different tissues. Results: The results showed that the expression of the above genes changed significantly in plants grown under eCO2 for 90 and 120 days as photosynthetic rate increased, while leaf and fruit sugar content decreased and the activity of four sucrose metabolism-related enzymes increased in leaves, and acid and neutral invertase increased in fruits. Furthermore, a significant correlation was detected between sugar content and enzyme activity. The expression of LBAI, LBSPS, and LBNI were high in stems, whereas LBSS was predominantly expressed in fruits. Conclusions: eCO2 affects chlorophyll, photosynthesis, sugars, and related enzyme activities in goji berry leaves and fruits after 90 and 120 days of treatment. Our findings provide fundamental data on photosynthesis and sugar accumulation trends in goji berries under eCO2 exposure.

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Section: Regulation Of Sucrose Metabolism-related Gene Expressionsupporting

confidence: 59%

Section: Regulation Of Sucrose Metabolism-related Gene Expressionmentioning

confidence: 96%

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WITHDRAWN: Effect of Elevated CO2 on Photosynthetic Accumulation and Sucrose Metabolism-related Enzyme Activity and Gene Expression Patterns in Goji Berry (Lycium Barbarum L.)

Xie

et al. 2020

Preprint

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“…Sucrose, glucose, and fructose are the main soluble sugars in the fruit of the plant, and the contents of these sugars play a key role in the quality of fruit. Research in goji berry and other fruit crops indicated a close relationship between fruit sugar accumulation and sucrose-metabolizing enzymes, including sucrose synthase, sucrose phosphate synthase, acid invertase, and neutral invertase [ 12 , 26 , 27 ]. In contrast to the sugar components response, a significant variation from 90 days to 120 days in the activity levels of sucrose metabolism-related enzymes was observed ( Figure 6 e–h).…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of Three Sugar Metabolism Genes (LBGAE, LBGALA, and LBMS) Regulated in Response to Elevated CO2 in Goji Berry (Lycium barbarum L.)

Devi

Reddy

et al. 2021

Plants

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The composition and content of sugar play a pivotal role in goji berry (Lycium barbarum L.) fruits, determining fruit quality. Long-term exposure of goji berry to elevated CO2 (eCO2) was frequently demonstrated to reduce sugar content and secondary metabolites. In order to understand the regulatory mechanisms and improve the quality of fruit in the changing climate, it is essential to characterize sugar metabolism genes that respond to eCO2. The objectives of this study were to clone full-length cDNA of three sugar metabolism genes—LBGAE (Lycium barbarum UDP-glucuronate 4-epimerase), LBGALA (Lycium barbarum alpha-galactosidase), and LBMS (Lycium barbarum malate synthase)—that were previously identified responding to eCO2, and to analyze sequence characteristics and expression regulation patterns. Sugar metabolism enzymes regulated by these genes were also estimated along with various carbohydrates from goji berry fruits grown under ambient (400 μmol mol−1) and elevated (700 μmol mol−1) CO2 for 90 and 120 days. Homology-based sequence analysis revealed that the protein-contained functional domains are similar to sugar transport regulation and had a high sequence homology with other Solanaceae species. The sucrose metabolism-related enzyme’s activity varied significantly from ambient to eCO2 in 90-day and 120-day samples along with sugars. This study provides fundamental information on sugar metabolism genes to eCO2 in goji berry to enhance fruit quality to climate change.

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“…Starch is the main carbohydrate stored, which allows them to meet their long-term carbon needs [69]. The main component of starch is synthesized by starch synthases (SS2), GBEs, and PYGs [70,71]. 'XBJ' highly expressed three PYGs and GBEs in each vernalization period.…”

Section: Gene Expression Changes In the Starch And Sucrose Metabolismmentioning

confidence: 99%

Comparative Transcriptome Analysis of Gene Expression and Regulatory Characteristics Associated with Different Vernalization Periods in Brassica rapa

Dai

Zhang

Sun

et al. 2020

Genes

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Brassica rapa is an important Chinese vegetable crop that is beneficial to human health. The primary factor affecting B. rapa yield is low temperature, which promotes bolting and flowering, thereby lowering its commercial value. However, quickened bolting and flowering can be used for rapid breeding. Therefore, studying the underlying molecular mechanism of vernalization in B. rapa is crucial for solving production-related problems. Here, the transcriptome of two B. rapa accessions were comprehensively analyzed during different vernalization periods. During vernalization, a total of 974,584,022 clean reads and 291.28 Gb of clean data were obtained. Compared to the reference genome of B. rapa, 44,799 known genes and 2280 new genes were identified. A self-organizing feature map analysis of 21,035 differentially expressed genes was screened in two B. rapa accessions, ‘Jin Wawa’ and ‘Xiao Baojian’. The analysis indicated that transcripts related to the plant hormone signal transduction, starch and sucrose metabolism, photoperiod and circadian clock, and vernalization pathways changed notably at different vernalization periods. Moreover, different expression patterns of TPS, UGP, CDF, VIN1, and seven hormone pathway genes were observed during vernalization between the two accessions. The transcriptome results of this study provide a new perspective on the changes that occur during B. rapa vernalization, as well as serve as an excellent reference for B. rapa breeding.

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Expression Patterns, Activities and Sugar Metabolism Regulation of Sucrose Phosphate Synthase, Sucrose Synthase, Neutral Invertase and Soluble Acid Invertase in Different Goji Cultivars during Fruit Development

Cited by 16 publications

References 24 publications

WITHDRAWN: Effect of Elevated CO2 on Photosynthetic Accumulation and Sucrose Metabolism-related Enzyme Activity and Gene Expression Patterns in Goji Berry (Lycium Barbarum L.)

WITHDRAWN: Effect of Elevated CO2 on Photosynthetic Accumulation and Sucrose Metabolism-related Enzyme Activity and Gene Expression Patterns in Goji Berry (Lycium Barbarum L.)

Cloning and Characterization of Three Sugar Metabolism Genes (LBGAE, LBGALA, and LBMS) Regulated in Response to Elevated CO2 in Goji Berry (Lycium barbarum L.)

Comparative Transcriptome Analysis of Gene Expression and Regulatory Characteristics Associated with Different Vernalization Periods in Brassica rapa

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Expression Patterns, Activities and Sugar Metabolism Regulation of Sucrose Phosphate Synthase, Sucrose Synthase, Neutral Invertase and Soluble Acid Invertase in Different Goji Cultivars during Fruit Development

Cited by 16 publications

References 24 publications

WITHDRAWN: Effect of Elevated CO2&nbsp;on Photosynthetic Accumulation and Sucrose Metabolism-related Enzyme Activity and Gene Expression Patterns in Goji Berry (Lycium Barbarum L.)

WITHDRAWN: Effect of Elevated CO2&nbsp;on Photosynthetic Accumulation and Sucrose Metabolism-related Enzyme Activity and Gene Expression Patterns in Goji Berry (Lycium Barbarum L.)

Cloning and Characterization of Three Sugar Metabolism Genes (LBGAE, LBGALA, and LBMS) Regulated in Response to Elevated CO2 in Goji Berry (Lycium barbarum L.)

Comparative Transcriptome Analysis of Gene Expression and Regulatory Characteristics Associated with Different Vernalization Periods in Brassica rapa

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WITHDRAWN: Effect of Elevated CO2 on Photosynthetic Accumulation and Sucrose Metabolism-related Enzyme Activity and Gene Expression Patterns in Goji Berry (Lycium Barbarum L.)

WITHDRAWN: Effect of Elevated CO2 on Photosynthetic Accumulation and Sucrose Metabolism-related Enzyme Activity and Gene Expression Patterns in Goji Berry (Lycium Barbarum L.)