Expression patterns and quantitative assessment of neurochemical markers in the lung of the gray bichir, Polypterus senegalus (Cuvier, 1829)

Lauriano, Eugenia Rita; Icardo, José M.; Zaccone, Daniele; Kuciel, Michał; Satora, Leszek; Alesci, Alessio; Alfa, Maria; Zaccone, Giacomo

doi:10.1016/j.acthis.2015.08.007

Cited by 22 publications

(21 citation statements)

References 33 publications

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“…Only previous studies (Lauriano et al, 2015;Porteus et al, 2015;Rimoldi et al, 2016;Zaccone et al, 2003) have reported the presence of nNOS in the neuroepithelial cells, the putative oxygen receptor of fish and their associated innervation in the gill, larval skin and respiratory gas bladder in fish. The role of gasotransmitters NO, hydrogen disulphide (H2S), carbon monoxide (CO) has been studied in carotid body O 2 sensing.…”

Section: Introductionmentioning

confidence: 99%

“…No study has yet assessed the role of NO in O 2 chemoreception in air-breathing fishes. Only previous studies (Lauriano et al, 2015;Porteus et al, 2015;Rimoldi et al, 2016;Zaccone et al, 2003) have reported the presence of nNOS in the neuroepithelial cells, the putative oxygen receptor of fish and their associated innervation in the gill, larval skin and respiratory gas bladder in fish. Porteus et al (2015) have also demonstrated that NO has an inhibitory effect on the hypoxic ventilatory response in adult zebrafish, but an excitatory effect on the hypoxic ventilation response of larvae.…”

Section: Introductionmentioning

confidence: 99%

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First demonstration of the neuroepithelial cells and their chemical code in the accessory respiratory organ and the gill of the sharptooth catfish, Clarias gariepinus: A preliminary study

et al. 2018

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Available studies that have examined O 2 sensing in fish have indicated that oxygensensitive neuroepithelial cells (NECs) are O 2 sensors in the gills and initiate cardiorespiratory reflexes in aquatic vertebrates. This is the first study describing the occurrence of NECs in accessory respiratory organs in the air-breathing catfish Clarias gariepinus. Immunocytochemical stainings with specific neuronal markers such as nNOS, VAchT, 5-HT and TH have been shown to be very useful for location and distribution of these cells in the gill fans and suprabranchial chamber that take origin from the transformation of the gill tissue. But the response of these putative O 2 chemoreceptors, their role in the respiratory reflexes and their innervation await investigation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First demonstration of the neuroepithelial cells and their chemical code in the accessory respiratory organ and the gill of the sharptooth catfish, Clarias gariepinus: A preliminary study

et al. 2018

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“…Numerous 5HT‐positive nerve fibers and enteroendocrine cells have been observed in the gut of the basal actinopterygian, Lepisosteus oculatus , and the euteleost, Clarias batrachus (Zaccone et al, ). In the polymorphous granular cells (PGCs) that find in the mucociliated epithelium of the lung in the air breathing fish Polypterus senegalus (Lauriano et al, ) has been demonstrated the simultaneous occurrence of piscidin 1 and 5‐HT, suggesting the involvement of serotonin in immune responses.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical colocalization of G protein alpha subunits and 5-HT in the rectal gland of the cartilaginous fishScyliorhinus canicula

et al. 2017

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Serotonin [5-hydroxytryptamine (5-HT)] is an important neuromodulator involved in a wide range of physiological functions. The effects of serotonin are mediated by an extended family of receptors coupled to multiple heterotrimeric G-proteins, associated with cellular membrane. G proteins connect receptors to effectors and thus trigger intracellular signaling pathways. These cellular processes several regulate systemic functions such as embryonic development, gonadal development, learning and memory, and organismal homeostasis. Generally, elasmobranch fish dwell a hypersaline environment and utilize a specialized extrarenal salt secreting organ, the rectal gland, to face ionic homeostasis. In this study in addition to the morphological, histochemical and immunohistochemical description of the Scyliorhinus canicula rectal gland, for the first time, the presence of serotonin (5-HT), and distribution of different types of G protein alpha subunits (Gα o, Gα q/11, and Gα s/olf) has been investigated in the rectal gland epithelium by confocal immunofluorescence techniques. Colocalization G proteins and 5-HT in the secretory epithelium of the gland suggests serotonin acts as a hormone and involves G proteins in an autocrine-paracrine control of rectal gland homeostasis.

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“…Neuroendocrine cells (NECs) lacking intraepithelial nerve endings (Zaccone, Goniakowska‐Witalinska, Lauweryns, Fasulo, & Tagliafierro, ) have been described in the ciliated areas of the polypterid lung (Zaccone et al, ; Zaccone, Mauceri, & Fasulo, ). Recently, granular cells and NECs have been identified on using immunohistochemistry and have been suggested to be implicated in the control of both circulation and respiration in bichirs (Lauriano et al, ; Zaccone et al, ). Recent works have also provided data on lung innervation (Lauriano et al, ; Zaccone, Mauceri, Maisano, & Fasulo, ) and vascularization (Longo, Riccio, & McCune, ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, granular cells and NECs have been identified on using immunohistochemistry and have been suggested to be implicated in the control of both circulation and respiration in bichirs (Lauriano et al, ; Zaccone et al, ). Recent works have also provided data on lung innervation (Lauriano et al, ; Zaccone, Mauceri, Maisano, & Fasulo, ) and vascularization (Longo, Riccio, & McCune, ). While many histological details of the polypterid lung appear to be well established, detailed structural systematic studies have never been performed.…”

Section: Introductionmentioning

confidence: 99%

The lungs of Polypterus senegalus and Erpetoichthys calabaricus: Insights into the structure and functional distribution of the pulmonary epithelial cells

Icardo

Colvée

Kuciel

et al. 2017

Journal of Morphology

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The present article is a comparative, structural study of the lung of Polypterus senegalus and Erpetoichthys calabaricus, two species representative of the two genera that constitute the Polypteriformes. The lung of the two species is an asymmetric, bi-lobed organ that arises from a slit-like opening in the ventral side of the pharynx. The wall is organized into layers, being thicker in P. senegalus. The inner epithelium contains ciliated and non-ciliated bands. The latter constitute the respiratory surface and are wider in E. calabaricus. The air-blood barrier is thin and uniform in P. senegalus and thicker and irregular in E. calabaricus. In the two species, the ciliated areas contain ciliated cells, mucous cells and cells with lamellar bodies. Additionally, P. senegalus contains polymorphous granular cells (PGCs) and neuroendocrine cells (NECs) while E. calabaricus lacks PGCs but shows granular leukocytes and a different type of NEC. Interestingly, ciliated cells and secretory cells show a dual morphology in E. calabaricus indicating the presence of cellular subtypes and suggesting more complex secretory activity. Also in E. calabaricus, cilia show a novel doublet-membrane interaction that may control the displacement of the microtubule doublets. The subepithelium is a connective layer that appears thicker in P. senegalus and contains, in the two species, fibroblasts and granulocytes. The outer layer contains bundles of richly innervated striated muscle. This layer is likely involved in the control of lung motion. In the two species, smooth muscle cells constitute a limiting layer between the subepithelium and the striated muscle compartment. The role of this layer is unclear.

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Expression patterns and quantitative assessment of neurochemical markers in the lung of the gray bichir, Polypterus senegalus (Cuvier, 1829)

Cited by 22 publications

References 33 publications

First demonstration of the neuroepithelial cells and their chemical code in the accessory respiratory organ and the gill of the sharptooth catfish, Clarias gariepinus: A preliminary study

First demonstration of the neuroepithelial cells and their chemical code in the accessory respiratory organ and the gill of the sharptooth catfish, Clarias gariepinus: A preliminary study

Immunohistochemical colocalization of G protein alpha subunits and 5-HT in the rectal gland of the cartilaginous fishScyliorhinus canicula

The lungs of Polypterus senegalus and Erpetoichthys calabaricus: Insights into the structure and functional distribution of the pulmonary epithelial cells

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