Expression patterns of Doppel gene in golden hamster: Quantification using real-time RT-PCR

Li, Y.R.; Li, Q.; Yang, Jianmin; Zhou, Xiangmei; Yin, Xiaonan; Zhao, Deming

doi:10.1016/j.mcp.2008.04.004

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…Real-time fluorescence quantitative RT-PCR for gene mRNA expression are reliable, and results are comparable and repeatable (Li et al, 2008). In the cited study, PrP mRNA expression was evaluated in the central and peripheral nervous tissues of golden hamster using the real-time quantitative fluorescent dye SYBR Green I, which could be incorporated into double-stranded DNA during PCR product formation.…”

Section: Discussionmentioning

confidence: 99%

“…In general, the ovine alleles VRQ and ARQ seem to be related to a higher susceptibility to scrapie, while the alleles ARR and AHQ are associated with lower susceptibility. Doppel mRNA expression in humans (Makrinou et al, 2002) and mice (Li et al, 2000) has been shown to have spatial and temporal patterns, as determined by Northern blot or in situ hybridization, and Doppel mRNA levels in golden hamster have been shown to be tissuespecific using real-time RT-PCR (Li et al, 2008). However, the expression patterns of the Doppel gene in differential ovine PRNP genotypes were previously unknown.…”

Section: Introductionmentioning

confidence: 99%

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Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR

Wang

Liu

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Doppel is a homologue of cellular prion protein (PrP)-like protein (PrP C ). Different tissue samples were collected from the central nervous system plus four regions of lymphoid system, eleven regions of digestive tract and two reproductive organs from four ARR/ARQ and four ARH/ARQ sheep, genotypes of the PrP gene. Total RNA was isolated from these samples, and Doppel mRNA was quantified by real-time RT-PCR using SYBR Green. Doppel mRNA expression was higher in the ovary, hypothalamus and brain than in other tissues, and it significantly differed between the two genotypes in brain, ileum, cecum, rectum, colon, and uterus. This study demonstrated that Doppel mRNA expression in sheep with ARR/ARQ or ARH/ARQ genotypes was very different. These findings could be helpful in future studies of the relationship between PrP and Doppel.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR

Wang

Liu

et al. 2015

Genet. Mol. Res.

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“…Since the invention of the flotation column, it has made great progress because of its wide adaptability in handling fine-grained materials and other scientific fields [4][5][6][7][8]. The gas parameters, such as gas retention rate, bubble diameter, and bubble surface area flux, are the most critical indexes to evaluate flotation, which directly affect the effectiveness of flotation columns [9][10][11]. Therefore, when controlling and diagnosing the operation of flotation columns, it is important to know the gas parameters.…”

Section: Introductionmentioning

confidence: 99%

Study of a Novel Fluidized Bed Flotation Column with Enhanced Bubble Dispersion

Yin,

Han,

Yang

et al. 2024

Minerals

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Flotation machines and flotation columns are widely used as separation equipment for coal sludge. In order to obtain better flotation results for different particle sizes, the bubble distribution and size in the flow field need to be studied. In this paper, a novel three-phase fluidized bed flotation column (TFC) was designed, and the shear effect of liquid velocity (0.198 m/s, 0.226 m/s, and 0.254 m/s) and static bed height (0.1 m, 0.2 m, and 0.3 m) on the bubbles in the mineralized flow field region was investigated and evaluated for the formation of bubbles by shear in laboratory and semi-industrial experiments. The results show that the increase in filling bed height has a very obvious strengthening effect on the reduction of bubble diameter, and after the filling bed height reaches a certain value, the filling bed height will weaken the effect of apparent gas velocity on bubble diameter. The apparent gas velocity has different influencing effects on bubble diameter, and under conditions of low water velocity, the increase in the apparent gas velocity contributes to the reduction of bubble diameter. The conclusions of this study are expected to optimize the operating parameters of the flotation mineralization process and enrich the study of TFC, which can provide a reference for the design of future TFC studies.

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“…two (instead of one as for PrP) disulphide bonds, and two N-glycosylation sites. Dpl expression is highest in the testis, where it is mainly involved in the male reproductive functions and its mRNA has also been found in the spleen, bone marrow, skeletal muscles, and heart [25].…”

Section: The Cellular Prion Protein and Its Structurementioning

confidence: 99%

Dimerization of the prion protein studied by site specific mutagenesis to p-benzoyl-L-phenylalanine

Sangeetham

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Expression patterns of Doppel gene in golden hamster: Quantification using real-time RT-PCR

Cited by 7 publications

References 22 publications

Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR

Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR

Study of a Novel Fluidized Bed Flotation Column with Enhanced Bubble Dispersion

Dimerization of the prion protein studied by site specific mutagenesis to p-benzoyl-L-phenylalanine

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