Expression Patterns of Poliovirus Receptor, Erythrocyte Protein Band 4.1-Like 3, Regulator of G-Protein Signaling 11, and Oxytocin Receptor in Mouse Ovarian Cells During Follicle Growth and Early Luteinization In Vitro and In Vivo1

Abstract

Poliovirus receptor (Pvr), erythrocyte protein band 4.1-like 3 (Epb4.1l3), regulator of G-protein signaling 11 (Rgs11), and oxytocin receptor (Oxtr) expression were quantified in in vitro- and in vivo-grown mouse follicles. The expression of all genes was increased during antral growth in in vitro-grown cumulus cells, whereas only Rgs11 and Oxtr were increased and Pvr and Epb4.1l3 were decreased in in vivo grown cumulus cells. In vivo mural granulosa cells showed the highest expression of Pvr, Rgs11, and Oxtr. The in vitro granulosa + theca compartment responded to human chorionic gonadotropinduring early luteinization by either an upregulation (Pvr, Oxtr) or downregulation (Epb41l3, Rgs11). Oocytes expressed Epb4.1l3, not Rgs11, and Pvr only in in vitro-grown oocytes. Translation into protein was confirmed for Epb4.1l3 in in vitro-grown follicles and in vivo-grown cumulus-oocyte complexes. Protein 4.1B was present during antral growth in cumulus, granulosa cells, and oocytes. Hypothetical functions of Epb4.1l3 and Pvr involve cell adhesion regulation and Rgs11 could be involved in cAMP production in the follicle. Oxtr is known to be important during and after the ovulatory stimulus, but, as in bovine, was also regulated during folliculogenesis. High expression of Pvr and Epb4.1l3 with culture duration in cumulus cells might mark inappropriate differentiation into a mural granulosa-like cell type and function as negative follicle development marker. Rgs11 and Oxtr are both in vivo and in vitro upregulated in cumulus cells during antral follicle growth and might be considered positive markers for follicle development.

New candidate genes to predict pregnancy outcome in single embryo transfer cycles when using cumulus cell gene expression

“…This may confirm the findings that a family member of CAMK1D, CAMK4, can increase the CYP11A1 expression in granulosa-luteal cells (20). The role of the three most promising genes in pregnancy prediction might be studied in an in vitro model (37), allowing observation and regulation during the follicular growth and post-LH phases.…”

Fertility preservation for social indications: a cost-based decision analysis

“…In vitro growth of follicles isolated from cryopreserved ovarian tissue, followed by in vitro maturation to produce oocytes competent for fertilization and implantation, is an active area of investigation for cancer patients (13)(14)(15)(16)(17)(18)(19)(20)(21)(22). Thus far, successful live birth has been achieved in murine models (23), and the technology is being optimized in nonhuman primates (19,(24)(25)(26)(27).…”

Gene expression differences induced by equimolar low doses of LH or hCG in combination with FSH in cultured mouse antral follicles

“…All three genes were not affected by gonadotropins. Absence of significant Oxtr, Pgr, and Egfr expression induction indicated that mural cells did not express early signs of luteinization (Fujinaga et al 1994, Clemens et al 1998, Segers et al 2012, which was reinforced by the endocrine profile on day 9.…”

“…35 pM rhCG, or 25 mIU/ml HP-hMG ('Menopur', Ferring). The 10 mIU/ml rFSH condition is a continuation of the minimal effective dose of FSH needed in culture (Adriaens et al 2004, Segers et al 2012. The 25 mIU/ml rFSH condition is considered 'slightly supraphysiological' in accordance with the supraphysiological FSH injections in assisted reproductive technologies (ART) patients .…”

“…Egfr mRNA and protein are downregulated in mouse cumulus Oxytocin receptor (Oxtr) G-protein-coupled receptor influencing steroidogenesis, ovulation, luteinization, and luteal regression (Gimpl & Fahrenholz 2001) Oxtr mRNA is induced in mouse mural granulosa cells but not in cumulus cells (Segers et al 2012) treatment rFSH10, rFSH25CLH, rFSH25ChCG, and HP-hMG25 (A); acute phase of treatment rFSH10 and rFSH25 (B); chronic phase of treatment rFSH10, rFSH25CLH, rFSH25ChCG, and HP-hMG25 (C); or chronic phase of treatment rFSH10 and rFSH25 (D). In each follicle culture experiment, follicles were harvested from the ovaries of several mice and randomly distributed to different culture plates, resulting in on average ten follicles per culture plate.…”