Expression patterns of WNT/β-CATENIN signaling molecules during human tooth development

Wang, Bingmei; Li, Hanliang; Liu, Ying; Xiao-qin, Lin; Lin, Yao; Wang, Ye; Hu, Xuefeng; Zhang, Yanding

doi:10.1007/s10735-014-9572-5

Cited by 49 publications

(44 citation statements)

References 41 publications

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“…Inset: details of individual cervical loops on the opposite sides of tooth germ with complete (1) and delayed (2) showing tendency to increase during development in the dental pulp and odontoblasts, but in the preameloblasts as well. Co-expression of CKs and vimentin seems to be a common phenomenon that occurs at brief periods of organogenesis with strictly regulated timeline, and it usually hallmarks epithelial-to-mesenchymal or mesenchymalto-epithelial transformation of developing tissues (Carev et al 2008;Wang et al 2014;Vukojevic et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Expression of cytokeratin 8, vimentin, syndecan-1 and Ki-67 during human tooth development

et al. 2014

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Spatio-temporal immunolocalizations of cytokeratin 8 (CK8), vimentin, syndecan-1 and Ki-67 were analyzed in ten human incisors and canine tooth germs between the 7th and 20th developmental weeks. CK8 expression was mild to moderate in the epithelial tooth parts, while it shifted from absent or mild in its mesenchymal parts, but few cells, sparsely distributed throughout the tooth germ, strongly expressed CK8. As development progressed, CK8 expression increased to strong in preameloblasts, while expression of vimentin increased to moderate in the epithelial and mesenchymal tooth parts, particularly in the dental papilla and sac. Co-expression of CK8 and vimentin was observed in some parts of the tooth germ, and was increasing in the differentiating preameloblasts and preodontoblasts. Syndecan-1 showed characteristic shift of expression from epithelial to mesenchymal tooth parts, being particularly strong in dental papilla, sac and cervical loops, while co-expression of Ki-67/syndecan-1 was strong in the dental papilla. Our study demonstrated spatio-temporal expression and restricted co-expression of the investigated markers, indicating participation of CK8 and vimentin in cell proliferation and migration, and differentiation of preodontoblasts and preameloblasts. Our data also suggest involvement of syndecan-1 in morphogenesis of the developing tooth crown and cervical loops, and together with CK8 and vimentin in differentiation of preameloblasts and preodontoblasts.

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Section: Discussionmentioning

confidence: 99%

Expression of cytokeratin 8, vimentin, syndecan-1 and Ki-67 during human tooth development

et al. 2014

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“…Several Wnt signaling components, including Wnt ligands, receptors, transducers, transcription factors, and antagonists, are expressed in the dental epithelium and mesenchyme during tooth development in humans and mice (Table 1) [38], [39]. Wnt 3, Wnt4, Wnt6, Wnt7b, and Wnt10b are expressed in the epithelium.…”

Section: Canonical Wnt Signaling Pathway Components In the Tooth And mentioning

confidence: 99%

Role of the Wnt signaling molecules in the tooth

Tamura

Nemoto

2016

Japanese Dental Science Review

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SummaryWnt signaling plays a central role in many processes during embryonic development and adult homeostasis. At least 19 types of Wnt ligands, receptors, transducers, transcription factors, and antagonists have been identified in mammals. Two distinct Wnt signaling pathways, the canonical signaling pathway and the noncanonical signaling pathway, have been described. Some Wnt signaling pathway components are expressed in the dental epithelium and mesenchyme during tooth development in humans and mice. Functional studies and experimental analysis of relevant animal models confirm the effects of Wnt signaling pathway on the regulation of developing tooth formation and adult tooth homeostasis. Mutations in some Wnt signaling pathway components have been identified in syndromic and non-syndromic tooth agenesis. This review provides an overview of progress in elucidating the role of Wnt signaling pathway components in the tooth and the resulting possibilities for therapeutic development.

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“…It is conceivable that the differences in human and mouse tooth development process could be a consequence of the differential expression of FGF4, FGF7, FGF8, and FGF9, because they are continuously expressed in the differentiating ameloblasts and odontoblasts at the differentiation stage. In addition to FGFs, multiple families of signaling molecules are also expressed in the developing human tooth, including SHH, WNTs, and BMPs (Dong et al 2014;Hu et al 2013;Lin et al 2007;Wang et al 2014). These genes also exhibited similar but slightly distinct expression patterns in the developing human tooth as compared to their counterparts in the mouse.…”

Section: The Differences Between Human and Mouse Tooth Morphology Cormentioning

confidence: 99%

Expression profile of critical genes involved in FGF signaling pathway in the developing human primary dentition

Huang

Chun-ni

et al. 2015

Histochem Cell Biol

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Mammalian tooth development is regulated by paracrine signal molecules of several conserved family interactions between epithelium and mesenchyme. The expression patterns and regulative roles of FGF signaling have been extensively studied in the mouse odontogenesis; however, that is not well known in human tooth development. In order to unveil the molecular mechanisms that regulate human tooth morphogenesis, we examined the expression patterns of the critical molecules involved in FGF signaling pathway in the developing human tooth germ by in situ hybridization, immunohistochemistry, and real-time RT-PCR, including FGF ligands, receptors, and intracellular transducer. We found overlapping but distinct expression pattern of FGF ligands and receptors in the different stages and components. Expression of FGF4, FGF7, FGF8, and FGF9 persists widespread in human tooth mesenchyme, which is quite different to that of in mouse. FGFR1 may be the major receptor in regulate mechanisms of FGF signals in human tooth development. Real-time RT-PCR indeed confirmed the results of in situ hybridization. Results of K-Ras, p-ERK1/2, p-p38, p-JNK, and p-PDK1 expression reveal spatial and temporal patterns of FGF signaling during morphogenesis and organogenesis of human tooth germ. Activity of the FGF signaling transducer protein in human tooth germ was much higher than that of in mouse. Our results provided important FGF singling information in the developing process, pinpoint to the domains where the downstream target genes of FGF signaling can be sought, and enlightened our knowledge about the nature of FGF signaling in human tooth germ.

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Expression patterns of WNT/β-CATENIN signaling molecules during human tooth development

Cited by 49 publications

References 41 publications

Expression of cytokeratin 8, vimentin, syndecan-1 and Ki-67 during human tooth development

Expression of cytokeratin 8, vimentin, syndecan-1 and Ki-67 during human tooth development

Role of the Wnt signaling molecules in the tooth

Expression profile of critical genes involved in FGF signaling pathway in the developing human primary dentition

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