2007
DOI: 10.1099/mic.0.2006/002642-0
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Expression, production and release of the Eis protein by Mycobacterium tuberculosis during infection of macrophages and its effect on cytokine secretion

Abstract: The eis gene of Mycobacterium tuberculosis has been shown to play a role in the survival of the avirulent Mycobacterium smegmatis within the macrophage. In vitro and in vivo analysis of Deis deletion mutants and complemented strains showed no effect on survival of M. tuberculosis in U-937 macrophages or in a mouse aerosol infection model, respectively. Further studies were done in an attempt to determine the role of eis in M. tuberculosis intracellular survival and to define a phenotypic difference between wil… Show more

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Cited by 54 publications
(61 citation statements)
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“…The biological function of Eis in Mtb has been the subject of recent interest (6,7,(22)(23)(24)(25)(26)(27)(28)(29). The biochemical and structural studies described in this report provide clear evidence for an unprecedented multiacetylation capability of Eis that inactivates AG antibiotics.…”
mentioning
confidence: 74%
“…The biological function of Eis in Mtb has been the subject of recent interest (6,7,(22)(23)(24)(25)(26)(27)(28)(29). The biochemical and structural studies described in this report provide clear evidence for an unprecedented multiacetylation capability of Eis that inactivates AG antibiotics.…”
mentioning
confidence: 74%
“…It is possible that Eis of M. tuberculosis has functions lacked by the homologous genes. Eis is a member of the GNAT superfamily of acetyltransferases (Samuel et al, 2007), and although there are some conserved motifs within these enzymes, they also have extensive sequence variation and a broad array of roles (Vetting et al, 2005). Comparative analyses of the mycobacterial orthologues may give insights into the regions of the M. tuberculosis gene that are important in host-pathogen interactions, and shed light on the function of M. tuberculosis Eis.…”
Section: Discussionmentioning
confidence: 99%
“…An eis deletion mutant induces higher levels of TNF-a and lower levels of IL-10 in monocytes than those induced by control strains (Samuel et al, 2007), suggesting that Eis induces secretion of IL-10, which is known to inhibit TNF-a production. However, the eis mutant shows no growth defect in a macrophage cell line or in mice (Samuel et al, 2007). Eis reduces TNF-a and IL-4 production by mitogen-stimulated human T-cells (Lella & Sharma, 2007), indicating that Eis affects T-cell function.…”
mentioning
confidence: 97%
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