Expression profile analysis based on DNA microarray for patients undergoing off-pump coronary artery bypass surgery

Abstract

Off-pump coronary artery bypass (OPCAB) surgery is the most effective treatment for coronary heart disease. The aim of this study was to explore the effects of OPCAB on the basis of the associated molecular mechanisms. GSE12486 expression profiles downloaded from the Gene Expression Omnibus database (GEO) were analyzed to identify the differentially expressed genes (DEGs). Principal component analysis (PCA) was conducted based on the expression profiles of DEGs. Function and pathway enrichment of upregulated DEGs was performed, followed by protein-protein interaction (PPI) network construction. Gene Set Enrichment Analysis (GSEA) was used for miRNA enrichment analysis based on expression profiles and prediction of their association with the disease. Cytoscape was applied to construct miRNA regulatory networks of DEGs. In total 64 DEGs were identified, including 63 upregulated and 1 downregulated gene. The first principal component in the PCA analysis was able to distinguish between pre- and post-OPCAB samples. Upregulated DEGs mainly enriched 20 Gene Ontology terms, such as chemokine activity, and 5 pathways including the chemokine signaling pathway. The constructed PPI network contained 234 edges and 55 nodes, and 10 upregulated hub nodes, including FBJ murine osteosarcoma viral oncogene homolog (FOS), were screened. A total of 36 miRNAs, including MIR-224 and MIR-7, were screened by GSEA enrichment analysis. A miRNA regulatory network including 176 edges and 97 nodes was constructed, showing the regulatory relationships between miRNAs and DEGs. For example, early growth response 2 (EGR2) was regulated by 8 miRNAs including MIR-150, MIR-142-3P, MIR-367 and MIR-224. The identified DEGs might play important roles in patients pre- and post-OPCAB surgery via the regulation of associated genes.

RelB/NF-κB links cell cycle transition and apoptosis to endometrioid adenocarcinoma tumorigenesis

“…GSEA (version 2.2.0) (Cambridge, MA, UK) is a powerful analysis tool for integrating gene expression data with gene sets to identify unified biological themes. 23 Significantly differentially expressed genes were verified by qRT-PCR and WB after identification via Z-score fold-change screening.…”

Circulating microRNA expression profiling and bioinformatics analysis of patients with coronary artery disease by RNA sequencing

“…Another report has shown that early growth response 2 (EGR2) is regulated by 8 microRNAs, including miR‐150. It might play important roles in patients before and after off‐pump coronary artery bypass (OPCAB) surgery via the regulation of associated genes . Many studies have shown that miR‐17‐5p is an important regulator in the G1/S phase cell cycle transition .…”

Exosomal MicroRNA for Detection of Cardiac Sarcoidosis

“…Among these, we selected several of the most affected miRNAs (miR-7-1-3p, miR-32-3p, miR-211-5p, and miR-548u-3p) for evaluation by qRT-PCR (normalized to miR-16) in the validation cohort. Notably, miR-7-1-3p and miR-211-5p are reported biomarkers of ischemic heart disease (11)(12)(13). The concentrations of these miRNAs were significantly different in all patients with sarcoidosis, taken collectively, relative to control subjects.…”

“…Exosomal contents offer a rich source of potential biomarkers and may reflect the underlying molecular mechanisms of disease (5). miR-7-1-3p and miR-211-5p have been shown to be markers of, and in the case of miR-7-1-3p, incriminated in the pathogenesis of ischemic cardiac disease (11)(12)(13). However, the role of miR-7 in the pathogenesis of CS, if any, remains to be determined.…”