Expression profile analysis of the polygalacturonase-inhibiting protein genes in rice and their responses to phytohormones and fungal infection

Lu, Liaoxun; Zhou, Fei; Zhou, Yong; Fan, Xiaolei; Ye, Shuifeng; Wang, Lei; Chen, Hao

doi:10.1007/s00299-012-1239-7

Cited by 21 publications

(19 citation statements)

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“…Earlier evaluation of cis -regulatory elements of various plant pgip genes in silico detected elements responsive to light, wounding, salicylic acid, abscisic acid, fungal elicitors, ethylene, and various other motifs with unknown functions (Kumar et al , 2009; Lu et al , 2012). The 1078bp sequence upstream of the Pglpgip1 ORF was analysed in silico for the identification of cis- elements and showed the presence of numerous important regulatory motifs.…”

Section: Resultsmentioning

confidence: 99%

Experimental and bioinformatic characterization of a recombinant polygalacturonase-inhibitor protein from pearl millet and its interaction with fungal polygalacturonases

Prabhu

Singh

Kolkenbrock

et al. 2014

Journal of Experimental Botany

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Section: Resultsmentioning

confidence: 99%

Experimental and bioinformatic characterization of a recombinant polygalacturonase-inhibitor protein from pearl millet and its interaction with fungal polygalacturonases

Prabhu

Singh

Kolkenbrock

et al. 2014

Journal of Experimental Botany

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“…PGIP expression is regulated through different transduction pathways [ 33 , 51 ], perhaps to precisely calibrate the response to a wide range of biotic and environmental cues, including pathogen infection [ 52 – 54 ]. It is also clear that plants may have evolved mechanisms that integrate various stress signals in order to mount a coordinated, coherent response [ 51 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular and Functional Characterization of a Polygalacturonase-Inhibiting Protein from Cynanchum komarovii That Confers Fungal Resistance in Arabidopsis

Liu

Zhou

et al. 2016

PLoS ONE

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Compliance with ethical standards: This study did not involve human participants and animals, and the plant of interest is not an endangered species.Polygalacturonase-inhibiting proteins (PGIPs) are leucine-rich repeat proteins that plants produce against polygalacturonase, a key virulence agent in pathogens. In this paper, we cloned and purified CkPGIP1, a gene product from Cynanchum komarovii that effectively inhibits polygalacturonases from Botrytis cinerea and Rhizoctonia solani. We found the expression of CkPGIP1 to be induced in response to salicylic acid, wounding, and infection with B. cinerea and R. solani. In addition, transgenic overexpression in Arabidopsis enhanced resistance against B. cinerea. Furthermore, CkPGIP1 obtained from transgenic Arabidopsis inhibited the activity of B. cinerea and R. solani polygalacturonases by 62.7–66.4% and 56.5–60.2%, respectively. Docking studies indicated that the protein interacts strongly with the B1-sheet at the N-terminus of the B. cinerea polygalacturonase, and with the C-terminus of the polygalacturonase from R. solani. This study highlights the significance of CkPGIP1 in plant disease resistance, and its possible application to manage fungal pathogens.

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“…Rice Actin1 was used as an internal control. qRT-PCR was performed with Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA) using SYBR Green I (TaKaRa, Japan) as previously described [42]. Three replicates were performed for the analysis of each gene.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of OsSWEET5 in Rice Causes Growth Retardation and Precocious Senescence

et al. 2014

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As a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized a SWEET gene named OsSWEET5 through its overexpression in rice. Heterologous expression assay indicated that OsSWEET5 encoded a galactose transporter in yeast. OsSWEET5-overexpressing plants displayed the phenotypes of growth retardation and precocious senescence at seedling stage. GC-MS analysis showed that the sugar levels were largely altered in the leaves of the OsSWEET5-overexpressing plants. Molecular analysis revealed that these phenotypes might be due to the transcriptional changes of the genes involved in sugar metabolism and transport. In addition, the transgenic plants showed a lower level of auxin with altered transcription of genes involved in auxin signaling and translocation pathways. However, no obvious phenotype was observed between the amiRNA-OsSWEET5 transgenic lines and WT plants, which could be a result of the functional redundancy of the galactose transporters in rice. Taken together, our findings suggest that OsSWEET5 plays a crucial role in regulating the crosstalk between sugar and auxin in rice.

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Expression profile analysis of the polygalacturonase-inhibiting protein genes in rice and their responses to phytohormones and fungal infection

Abstract: All the seven Ospgip genes showed variable expression patterns in Minghui 63 and their expressions were regulated by different phytohormone treatments or fungal infection in Minghui 63 and Zhonghua 11.

Cited by 21 publications

References 50 publications

Experimental and bioinformatic characterization of a recombinant polygalacturonase-inhibitor protein from pearl millet and its interaction with fungal polygalacturonases

Experimental and bioinformatic characterization of a recombinant polygalacturonase-inhibitor protein from pearl millet and its interaction with fungal polygalacturonases

Molecular and Functional Characterization of a Polygalacturonase-Inhibiting Protein from Cynanchum komarovii That Confers Fungal Resistance in Arabidopsis

Overexpression of OsSWEET5 in Rice Causes Growth Retardation and Precocious Senescence

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