Expression profile and function analysis of MsCSP17 and MsCSP18 in the larval development of Mythimna separata

Chen, Tingting; Zhou, Xue; Wu, Jing; Xing, Gaoliang; Cao, Haiqun; Huang, Yong

doi:10.1017/s0007485323000354

Cited by 4 publications

(1 citation statement)

References 34 publications

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“…1 With these sensitive olfactory systems, they can precisely recognize various volatile molecules in the external environment, enabling them to complete a variety of essential behavioral responses including foraging, mating, spawning, avoiding natural enemies and so on. 2,3 Usually, volatile molecules from the environment are diffused through sensory lymph into holes in the insect's antennae, where they precisely bind to the corresponding odorant binding protein (OBP) and are then transported to specific olfactory receptors (Fig. S1).…”

Section: Introductionmentioning

confidence: 99%

Molecular recognition between volatile molecules and odorant binding proteins 7 by homology modeling, molecular docking and molecular dynamics simulation

Wang,

Duan,

Zhao

et al. 2024

J Sci Food Agric

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BACKGROUNDOdorant‐binding proteins (OBPs) in insects are key to detection and recognition of external chemical signals associated with survival. OBP7 in Spodoptera frugiperda's larval stage (SfruOBP7) may search for host plants by sensing plant volatiles, which are important sources of pest attractants and repellents. However, the atomic‐level basis of binding modes remains elusive.RESULTSSfruOBP7 structure was constructed through homology modeling, and complex models of six plant volatiles ((E)‐2‐hexenol, α‐pinene, (Z)‐3‐hexenyl acetate, lauric acid, O‐cymene and 1‐octanol) and SfruOBP7 were obtained through molecular docking. To study the detailed interactions between the six plant volatile molecules and SfruOBP7, we conducted three 300 ns molecular dynamics simulations for each study object. The correlation coefficients between binding free energy obtained by molecular mechanics/generalized Born surface area together with solvated interaction energy methods and experimental values are 0.90 and 0.88, respectively, showing a good correlation. By comparing binding free energy along with interaction patterns between SfruOBP7 and the six volatile molecules, hotspot residues of SfruOBP7 when binding with different volatile molecules were determined. Hydrophobic interactions stemming from van der Waals interactions play a significant role in SfruOBP7 and these plant volatile systems.CONCLUSIONThe optimized three‐dimensional structure of SfruOBP7 and its binding modes with six plant volatiles revealed their interactions, thus providing a means for estimating the binding energies of other plant volatiles. Our study will help to guide the rational design of effective and selective insect attractants. © 2024 Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Molecular recognition between volatile molecules and odorant binding proteins 7 by homology modeling, molecular docking and molecular dynamics simulation

Wang,

Duan,

Zhao

et al. 2024

J Sci Food Agric

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CYP4G subfamily genes mediate larval integument development in Spodoptera frugiperda

Zhou,

Yuan,

et al. 2024

Journal of Economic Entomology

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Cytochrome P450 (CYP) 4G subfamily is closely related to the synthesis of cuticular hydrocarbons, leading to the enhanced desiccation and insecticide resistance of pests. However, functions of CYP4Gs in larval integument development remain unknown in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), which is a major transboundary migratory pest and become a common pest in China. On the basis of the genome and transcriptome datasets of S. frugiperda, CYP4G74, CYP4G75, CYP4G108, and CYP4G109 were identified, which contained the conserved domains of P450s and CYP4Gs. The spatial and temporal expression analysis showed that CYP4G74 and CYP4G75 were significantly highly expressed in adults and larval integuments, while CYP4G108 and CYP4G109 had low expressions in larval integuments. After silencing CYP4G74 and CYP4G75 by RNA interference, abnormal integument development occurred in larvae, some of which became smaller and dead, indicating important roles of CYP4G74 and CYP4G75 in the synthesis and development of integuments. The results clarify the functions of CYP4Gs in S. frugiperda and provide potential targets for the control of this pest.

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A larval expressed chemosensory protein involved in recognition of anthocyanins in Spodoptera litura (Lepidoptera: Noctuidae)

Dong,

Huang,

Pei

et al. 2024

Journal of Economic Entomology

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Anthocyanins are secondary metabolites which act as diverse functions during plant growth. Insects can discriminate host plants by their sensitive gustatory systems. It is hypothetical that chemosensory proteins (CSPs) play a crucial role in regulating this behavioral process. However, the underlying molecular mechanisms remain obscure. In the present study, we characterized a CSP SlitCSP8 from the Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Quantitative real-time-polymerase chain reaction analysis demonstrated that SlitCSP8 was mainly expressed in the head of the 7th S. litura larvae, especially labrum. Further, recombinant SlitCSP8 was obtained using bacterial expression system. Fluorescence competitive binding assays demonstrated that the purified SlitCSP8 exhibited a strong binding affinity to anthocyanins, a natural compound derived from the host plant. Silencing SlitCSP8 through RNAi significantly reduced the sensitivity of S. litura larvae to anthocyanins-treated leaf disks, the development from larva to pupae was not affected. These data provide insight into the molecular basis that CSP8 can detect anthocyanins in host plants by chemosensory system of insects. It can be further used in designing novel optimal food attractant targeting to the CSPs for pest control.

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Expression profile and function analysis of MsCSP17 and MsCSP18 in the larval development of Mythimna separata

Cited by 4 publications

References 34 publications

Molecular recognition between volatile molecules and odorant binding proteins 7 by homology modeling, molecular docking and molecular dynamics simulation

Molecular recognition between volatile molecules and odorant binding proteins 7 by homology modeling, molecular docking and molecular dynamics simulation

CYP4G subfamily genes mediate larval integument development in Spodoptera frugiperda

A larval expressed chemosensory protein involved in recognition of anthocyanins in Spodoptera litura (Lepidoptera: Noctuidae)

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