Expression Profile in Omental and Subcutaneous Adipose Tissue from Lean and Obese Subjects. Repression of Lipolytic and Lipogenic Genes

Pozo, Carmen Hurtado del; Calvo, Rosa; Vesperinas-García, Gregorio; Gómez-Ambrosi, Javier; Frühbeck, Gema; Rubio, Miguel A.; Obregón, Marı́a Jesús

doi:10.1007/s11695-010-0246-4

Cited by 25 publications

(8 citation statements)

References 56 publications

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“…In support of previous studies (15), we showed that the expression of adipogenic and lipogenic genes was down-regulated with obesity in SAT of both black and white women. Decreased expression of these genes may represent an adaptive process limiting further accumulation of fat mass.…”

Section: Discussionsupporting

confidence: 91%

Reduced Gluteal Expression of Adipogenic and Lipogenic Genes in Black South African Women Is Associated with Obesity-Related Insulin Resistance

Goedecke

Evans

Keswell

et al. 2011

The Journal of Clinical Endocrinology & Metabolism

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Context Black South African women are less insulin sensitive than their white counterparts, despite less central and greater peripheral fat deposition. We hypothesized that this paradox may be explained, in part, by differences in the adipogenic capacity of subcutaneous adipose tissue (SAT). Objective To measure adipogenic and lipogenic gene expression in abdominal and gluteal SAT depots, and determine their relationships with insulin sensitivity (SI) in South African women. Design Cross-sectional. Participants 14 normal-weight (BMI <25 kg/m2) black, 13 normal-weight white, 14 obese (BMI >30 kg/m2) black and 13 obese white premenopausal South African women. Main outcomes SI (frequently sampled intravenous glucose tolerance test) in relation to expression of adipogenic and lipogenic genes in abdominal and gluteal SAT depots. Results With increasing BMI, black women had less visceral fat (P=0.03) and more abdominal (P=0.017) and gynoid (P=0.041) SAT but had lower SI (P<0.01) than white women. The expression of adipogenic and lipogenic genes was proportionately lower with obesity in black, but not white women in the gluteal and deep SAT depots (P<0.05 for ethnicity x BMI effect). In black women only, the expression of these genes correlated positively with SI (all P<0.05), independently of age and fat mass. Conclusions Obese black women have reduced SAT expression of adipogenic and lipogenic genes compared to white women, which associates with reduced SI. These findings suggest that obesity in black women impairs SAT adipogenesis and storage, potentially leading to insulin resistance and increased risk of type 2 diabetes.

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Section: Discussionsupporting

confidence: 91%

Reduced Gluteal Expression of Adipogenic and Lipogenic Genes in Black South African Women Is Associated with Obesity-Related Insulin Resistance

Goedecke

Evans

Keswell

et al. 2011

The Journal of Clinical Endocrinology & Metabolism

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“…This opposite regulation might have arisen as a consequence of the adipose tissue expansion limit, which once reached, will reduce the capacity of the adipose tissue to store energy efficiently, and lipids begin to accumulate in other tissues such as the liver, contributing to lipotoxicity and associated pathologies (insulin resistance, diabetes or fatty liver) [28]. In a recent study with adipose tissues from obese subjects we found a generalized decrease in the expression of enzymes involved in lipogenesis, as well as in lipolysis, [29], both likely due to a defense mechanism. Regarding lipolysis, the mRNA expression of two enzymes, ADCY3 and ATGL, were assayed in the liver and adipose tissues samples of our present study.…”

Section: Discussionmentioning

confidence: 90%

ChREBP expression in the liver, adipose tissue and differentiated preadipocytes in human obesity

Pozo

Vesperinas-García

Rubio

et al. 2011

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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ChREBP is an essential transcription factor for lipogenesis. Its physiological role in adipose tissue has been studied only to a small extent and the control of its expression remains unknown in human adipocytes. We have studied ChREBP mRNA and protein expression levels in the liver and the omental (OM) and subcutaneous (SC) adipose tissues from obese and lean subjects, as well as in human differentiated preadipocytes. Liver and OM and SC adipose tissue biopsies were obtained from lean and obese patients. Human preadipocytes were isolated from the adipose tissues from obese patients and differentiated under adipogenic conditions. ChREBP expression levels were quantified by RT-PCR and Western blot analysis. We found opposing results in terms of ChREBP regulation in the liver and adipose samples. ChREBP increased in the liver from obese compared to lean subjects, whereas the expression decreased in both adipose tissues. The mRNAs of other adipogenic markers were checked in these tissues. The pattern of FASN was similar to the one for ChREBP, ADCY3 decreased in both adipose tissues from obese patients, AP2 decreased only in OM adipose tissue of obese patients and ATGL did not change. The levels of ChREBP mRNA and protein showed dramatic increases during the differentiation of human OM and SC preadipocytes. In conclusion, ChREBP expression has an opposite regulation in the liver and adipose tissue from obese subjects which is compatible with the increased hepatic lipogenesis and decreased adipocytic lipogenesis found in these patients. The dramatic increase of ChREBP mRNA and protein levels during preadipocyte differentiation suggests a role in adipogenesis.

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“…miRNAs are a group of diverse regulatory molecules with potential to target hundreds of genes [30] and integrate complex molecular regulatory networks. Studies have focused on identifying differential expression of miRNAs in adipose of individuals submitted to opposite conditions such as low fat vs. high fat diet treatments [31] or lean vs. obese individuals [32] which reveals only particular aspects of miRNA biology in adipose tissue. However, in this study we aimed to perform a more holistic approach by focusing on the genomic context features of miRNA genes and their global expression under physiological adipose tissue conditions.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs in bovine adipogenesis: genomic context, expression and function

Romao

Jin

et al. 2014

BMC Genomics

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BackgroundMicroRNAs (miRNAs) are small non-coding RNAs found to regulate several biological processes including adipogenesis. Understanding adipose tissue regulation is critical for beef cattle as fat is an important determinant of beef quality and nutrient value. This study analyzed the association between genomic context characteristics of miRNAs with their expression and function in bovine adipose tissue. Twenty-four subcutaneous adipose tissue biopsies were obtained from eight British-continental crossbred steers at 3 different time points. Total RNA was extracted and miRNAs were profiled using a miRNA microarray with expression further validated by qRT-PCR.ResultsA total of 224 miRNAs were detected of which 155 were expressed in all steers (n = 8), and defined as the core miRNAs of bovine subcutaneous adipose tissue. Core adipose miRNAs varied in terms of genomic location (59.5% intergenic, 38.7% intronic, 1.2% exonic, and 0.6% mirtron), organization (55.5% non-clustered and 44.5% clustered), and conservation (49% highly conserved, 14% conserved and 37% poorly conserved). Clustered miRNAs and highly conserved miRNAs were more highly expressed (p < 0.05) and had more predicted targets than non-clustered or less conserved miRNAs (p < 0.001). A total of 34 miRNAs were coordinately expressed, being part of six identified relevant networks. Two intronic miRNAs (miR-33a and miR-1281) were confirmed to have coordinated expression with their host genes, transcriptional factor SREBF2 and EP300 (a transcriptional co-activator of transcriptional factor C/EBPα), respectively which are involved in lipid metabolism, suggesting these miRNAs may also play a role in regulation of bovine lipid metabolism/adipogenesis. Furthermore, a total of 17 bovine specific miRNAs were predicted to be involved in the regulation of energy balance in adipose tissue.ConclusionsThese findings improve our understanding on the behavior of miRNAs in the regulation of bovine adipogenesis and fat metabolism as it reveals that miRNA expression patterns and functions are associated with miRNA genomic location, organization and conservation.

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Expression Profile in Omental and Subcutaneous Adipose Tissue from Lean and Obese Subjects. Repression of Lipolytic and Lipogenic Genes

Cited by 25 publications

References 56 publications

Reduced Gluteal Expression of Adipogenic and Lipogenic Genes in Black South African Women Is Associated with Obesity-Related Insulin Resistance

Reduced Gluteal Expression of Adipogenic and Lipogenic Genes in Black South African Women Is Associated with Obesity-Related Insulin Resistance

ChREBP expression in the liver, adipose tissue and differentiated preadipocytes in human obesity

MicroRNAs in bovine adipogenesis: genomic context, expression and function

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