Expression profile of carp IFN correlate with the up-regulation of interferon regulatory factor-1 (IRF-1) in vivo and in vitro: the pivotal molecules in antiviral defense

Shan, Shijuan; Qi, Chenchen; Zhu, Yuying; Li, Hua; An, Liguo

doi:10.1016/j.fsi.2016.03.019

Cited by 33 publications

(35 citation statements)

References 52 publications

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“…IRF1 is constitutively expressed in different organs. When stimulated by poly(I:C), a molecular species associated with viral infection, peripheral blood leukocytes exhibit upregulation of IRF1 . IRF5 is a key molecule in antiviral and antibacterial immunity.…”

Section: Antiviral and Antibacterial Immunitymentioning

confidence: 99%

Molecular mechanisms underlying stress response and adaptation

Sun

Zhou

2017

Thoracic Cancer

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Environmental stresses are ubiquitous and unavoidable to all living things. Organisms respond and adapt to stresses through defined regulatory mechanisms that drive changes in gene expression, organismal morphology, or physiology. Immune responses illustrate adaptation to bacterial and viral biotic stresses in animals. Dysregulation of the genotoxic stress response system is frequently associated with various types of human cancer. With respect to plants, especially halophytes, complicated systems have been developed to allow for plant growth in high salt environments. In addition, drought, waterlogging, and low temperatures represent other common plant stresses. In this review, we summarize representative examples of organismal response and adaptation to various stresses. We also discuss the molecular mechanisms underlying the above phenomena with a focus on the improvement of organismal tolerance to unfavorable environments.

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Section: Antiviral and Antibacterial Immunitymentioning

confidence: 99%

Molecular mechanisms underlying stress response and adaptation

Sun

Zhou

2017

Thoracic Cancer

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“…Inflammation is a common and important physiological process that is activated in response to infections, such as bacteria and virus, of the body surface and organs of body surfaces, and organs in mammals . Many common lesions and frequently occurring diseases, such as boils, carbuncles, pneumonia, hepatitis, and nephritis, are associated with inflammation .…”

Section: Introductionmentioning

confidence: 99%

Targeted inhibition of histone deacetylase 6 in inflammatory diseases

Ran

Zhou

2019

Thoracic Cancer

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Targeting epigenetic modification of gene expression represents a promising new approach under investigation for the treatment of inflammatory diseases. Accumulating evidence suggests that epigenetic mechanisms, such as histone modification, play a crucial role in a number of inflammatory diseases, including rheumatoid arthritis, asthma, and contact hypersensitivity. Consistent with this role, histone deacetylase (HDAC) inhibitors have shown efficacy in the treatment of inflammatory diseases. In particular, selective inhibitors of HDAC6, a cytoplasmic member of the HDAC family that contains two deacetylase domains, are under investigation as a potential treatment strategy for inflammatory diseases due to their ability to regulate inflammatory cells and cytokines. Here, we review recent findings highlighting the critical roles of HDAC6 in a variety of inflammatory diseases, and discuss the therapeutic potential of HDAC6 inhibitors in these settings.

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“…IRFs play critical roles in antiviral and antibacterial immunity. Previous studies in C. carpio have shown that the expression of irf1 , irf3 , irf5 and irf7 was up‐regulated upon stimulation with poly I:C, viruses and bacteria (Feng et al, ; Shan et al, ; Zhu et al, ). We selected the spleen and head kidney in the present study, as these organs represent lymphoid and myeloid tissues, which are important in fish immune system.…”

Section: Discussionmentioning

confidence: 97%

Molecular characterization and functional analysis of interferon regulatory factor 9 (irf9) in common carp Cyprinus carpio: a pivotal molecule in the Ifn response against pathogens

Zhu

Shan

et al. 2019

Journal of Fish Biology

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In the present study, interferon (IFN) regulatory factor (IRF) 9 gene (irf9) was identified and characterized in common carp Cyprinus carpio. The predicted protein sequence of Irf9 contains a DNA binding domain (DBD) that possess five tryptophans, an IRF association domain (IAD) and two nuclear localisation signals (NLS). Alignment of Irf9 of C. carpio with the corresponding Irf9 proteins of other species showed that the DBD is more highly conserved than the IAD. The putative Irf9 protein sequence of C. carpio shares higher identities with teleosts (53.8–82.3%) and lower identities with mammals (30.2–31.0%). Phylogenetic studies of the putative amino‐acid sequence of IRF9 based on the neighbour‐joining method showed that Irf9 of C. carpio has the closest relationship with the grass carp Ctenopharyngodon idella. Tissue distribution analysis showed that irf9 transcripts were detectable in all examined tissues with the highest expression in the skin and the lowest expression in the head kidney. Poly I:C and Aeromonas hydrophila stimulation up‐regulated irf9 expression in the spleen, head kidney, foregut and hindgut at different time intervals. In addition, irf9 was induced by Poly I:C and lipopolysaccharides (LPS) in vitro. These results indicate that Irf9 participates in antiviral and antibacterial immunity. Transfection of irf9 up‐regulated the expression of cytokines, including type I IFN, protein kinase R (PKR), interferon‐stimulated gene (ISG)15 and tumour necrosis factor (TNF)α in epithelioma papulosum cyprini cells (EPC) upon poly I:C and LPS stimulation. A dual‐luciferase reporter assay revealed that Irf9 has no effect on NF‐κB activation. The present study on Irf9 provides new insights into the IFN system of C. carpio and a valuable experimental platform for future studies on the immune system of fish.

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Expression profile of carp IFN correlate with the up-regulation of interferon regulatory factor-1 (IRF-1) in vivo and in vitro: the pivotal molecules in antiviral defense

Cited by 33 publications

References 52 publications

Molecular mechanisms underlying stress response and adaptation

Molecular mechanisms underlying stress response and adaptation

Targeted inhibition of histone deacetylase 6 in inflammatory diseases

Molecular characterization and functional analysis of interferon regulatory factor 9 (irf9) in common carp Cyprinus carpio: a pivotal molecule in the Ifn response against pathogens

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