Expression profile of circular RNA in rat intimal hyperplasia and target gene prediction

Li, Tao; Rong, Zhihua; Chang, Neng-Bin; Liu, Xing; Xu, Jia‐Ying; Liu, Dong; Shi, Congcong; Zhang, Wenyi; Jiang, Rui; Jiang, Jun

doi:10.1002/jcp.28164

Cited by 4 publications

(3 citation statements)

References 39 publications

(50 reference statements)

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“…The rat CCA intimal hyperplasia model was made by balloon injury. 11,12 14 days after the procedure, the animals were sacrificed and CCAs were harvested. Histological sections showed marked neointima formed in CCA.…”

Section: Results Circdcbld1 Was Highly Expressed In Injured Arterymentioning

confidence: 99%

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Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1

Rong

Chang

Yao

et al. 2019

Molecular Therapy - Nucleic Acids

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We replicated the rat common carotid artery (CCA) intima hyperplasia model and found the expression of a circular RNA, circRNA_009723 (circDcbld1), was markedly increased in the CCA with intimal hyperplasia. In vitro, the suppression of circDcbld1 in rat vascular smooth muscle cells (VSMCs) led the increase of contractile smooth muscle cell markers and the decrease of cell migration. In vivo, the injection of chemically modified circDcbld1 small interfering RNA (siRNA) lessened the formation of neointima in rat CCA after balloon injury. Further experiments proved that circDcbld1, as a competing endogenous RNA, interacted with miR-145-3p and upregulated the level of neuropilin-1 (Nrp1), thereby regulating the migration of VSMCs. In this study, we demonstrated a new mechanism by which circular RNA promotes intimal hyperplasia. We deem that intervention in the circDcbld1-miR-145-3p/Nrp1 pathway might be a feasible approach to alleviate the post-injury intimal hyperplasia.

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Section: Results Circdcbld1 Was Highly Expressed In Injured Arterymentioning

confidence: 99%

“…The Detection of circRNA As previously described, 11 the total RNA of CCA was extracted, and the linear RNAs were degraded with exonuclease (Thermo Fisher Scientific, Waltham, MA, USA). circRNAs in samples were detected using Arraystar rat circRNA array (8 Â 15K; Kangchen Biotech, Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1

Rong

Chang

Yao

et al. 2019

Molecular Therapy - Nucleic Acids

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“…Being closed loops, circRNAs are not easily degraded by exonucleases. In addition, the half-life of circRNAs is doubled that of linear RNAs [18]. CircRNAs are reproducibly and easily detected in clinical blood samples.…”

Section: Discussionmentioning

confidence: 99%

Expression Profile Analysis of Differentially Expressed Circular RNAs in Steroid-Induced Osteonecrosis of the Femoral Head

Zhu

et al. 2019

Disease Markers

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Background. A growing number of studies have suggested that circular RNAs (circRNAs) serve as potential diagnostic biomarkers in many diseases. However, the role of circRNAs in steroid-induced osteonecrosis of the femoral head (SONFH) has not been reported. Methods. Secondary sequencing was performed to profile circRNA expression in peripheral blood samples from three SONFH patients and three healthy individuals. We confirmed our preliminary findings by qRT-PCR. Bioinformatics analysis was conducted to predict their functions. Results. The result showed 345 dysregulated circRNAs. qRT-PCR of eight selected circRNAs preliminarily confirmed the results, which were consistent with RNA sequencing. Bioinformatics analyses were performed to predict the functions of circRNAs to target the genes of miRNAs and the networks of circRNA-miRNA-mRNA interactions. Conclusions. This study provides a new and fundamental circRNA profile of SONFH and a theoretical basis for further studies on the functions of circRNAs in SONFH.

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Circular RNAs in the pathogenesis of atherosclerosis

Cao

Guo

et al. 2020

Life Sciences

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Expression profile of circular RNA in rat intimal hyperplasia and target gene prediction

Cited by 4 publications

References 39 publications

Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1

Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1

Expression Profile Analysis of Differentially Expressed Circular RNAs in Steroid-Induced Osteonecrosis of the Femoral Head

Circular RNAs in the pathogenesis of atherosclerosis

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