Expression profile of circular RNAs in cystic echinococcosis pericystic tissue

Kalifu, Baheti; Maitiseyiti, Abulaihaiti; Ge, Xiaohu; Chen, Xiong; Yuan, Meng

doi:10.1002/jcla.23687

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…Differentially expressed miRNAs (miR-181, miR-30, miR-365, miR-378, miR-449, and miR-16) that were identified in the sheep intestine, liver, and serum, as well as in mouse macrophages, following infection with E. granulosus may be critically involved in the body's immune response [ 30 – 32 ]. Microarray analysis of circRNA expression profiles in adjacent tissues of CE-infected patients showed that hsa_circRNA_006773, hsa_circRNA_049637, hsa_circRNA_104349, and hsa_circRNA_406281 might serve as CE prognostic biomarkers and therapeutic targets [ 33 ]. Interestingly, lncRNA regulates lipolysis and metabolic remodeling in E. granulosus –infected mice [ 34 ].…”

Section: Analysis and Discussion Of Clinical Datamentioning

confidence: 99%

[Retracted] Coding and Noncoding RNA Expression Profiles of Spleen CD4+ T Lymphocytes in Mice with Echinococcosis

Yang

Wang

et al. 2022

Contrast Media & Molecular Imaging

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Cystic echinococcosis (CE) is a severe and neglected zoonotic disease that poses health and socioeconomic hazards. So far, the prevention and treatment of CE are far from meeting people's ideal expectations. Therefore, to gain insight into the prevention and diagnosis of CE, we explored the changes in RNA molecules and the biological processes and pathways involved in these RNA molecules as E. granulosus infects the host. Interferon (IFN)-γ, interleukin (IL)-2, IL-4, IL-6, IL-10, IL-17A, and tumor necrosis factor (TNF)-α levels in peripheral blood serum of E. granulosus infected and uninfected female BALB/c mice were measured using the cytometric bead array mouse Th1/Th2/Th17 cytokine kit. mRNA, microRNA (miRNA), long noncoding RNA (lncRNA), and circular RNA (circRNA) profiles of spleen CD4+ T cells from the two groups of mice were analyzed using high-throughput sequencing and bioinformatics. The levels of IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNF-α were significantly higher in the serum of the CE mice than in control mice (P < 0.01). In total, 1,758 known mRNAs, 37 miRNAs, 175 lncRNAs, and 22 circRNAs were differentially expressed between infected and uninfected mice (|fold change| ≥ 0.585, P < 0.05). These differentially expressed molecules are involved in chromosome composition, DNA/RNA metabolism, and gene expression in cell composition, biological function, and cell function. Moreover, closely related to the JAK/STAT signaling pathways, mitogen-activated protein kinase signaling pathways, P53 signaling pathways, PI3K/AKT signaling pathways, cell cycle, and metabolic pathways. E. granulosus infection significantly increased the levels of IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNF-α in mouse peripheral blood of mice and significantly changed expression levels of various coding and noncoding RNAs. Further study of these trends and pathways may help clarify the pathogenesis of CE and provide new insights into the prevention and treatment of this disease.

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Section: Analysis and Discussion Of Clinical Datamentioning

confidence: 99%

[Retracted] Coding and Noncoding RNA Expression Profiles of Spleen CD4+ T Lymphocytes in Mice with Echinococcosis

Yang

Wang

et al. 2022

Contrast Media & Molecular Imaging

View full text Add to dashboard Cite

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“…Differentially expressed miRNAs (miR-181, miR-30, miR-365, miR-378, miR-449, miR-99, miR-130, and miR-16) that were identi ed in the sheep intestine, liver, and serum, as well as in mouse macrophages, following infection with E. granulosus may be critically involved in the body's immune response [29][30][31][32][33][34][35][36][37][38][39] . Microarray analysis of circRNA expression pro les in adjacent tissues of CE-infected patients, showed that hsa_circRNA_006773, hsa_circRNA_049637, hsa_circRNA_104349, and hsa_circRNA_406281 might serve as CE prognostic biomarkers and therapeutic targets [40] . Our high-throughput sequencing data are consistent with previous results showing that miR-29c-3p and miR-30b-5p are signi cantly downregulated in CE [41] .…”

Section: Discussionmentioning

confidence: 99%

Coding and Noncoding RNA Expression Profiles of Spleen CD4+ T Lymphocytes in Mice With Echinococcosis

Yang

Wang

et al. 2021

Preprint

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Background: Cystic echinococcosis (CE) is a severe and neglected zoonotic disease, which is caused by Echinococcus granulosus sensu lato and poses health and socioeconomic hazards. RNA molecules play important roles in genetic coding, translation, regulation, and gene expression and are classified into noncoding RNAs, such as long noncoding RNAs (lncRNAs), miRNAs, and circular RNAs (circRNAs), and coding RNAs (mRNAs) based on whether they encode proteins.Methods: Peripheral blood serum from E. granulosus-infected and uninfected female BALB/c mice was used to measure IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNF-α levels using the cytometric bead array mouse Th1/Th2/Th17 cytokine kit. mRNA, lncRNA, miRNA, and circRNA profiles were analyzed in spleen CD4+ T cells from the two groups of mice using high-throughput sequencing.Results: The results showed that the levels of serum IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNF-α were significantly higher in the CE mice than in the control group (P < 0. 01). A total of 1,758 known mRNAs, 37 miRNAs, 175 lncRNAs, and 22 circRNAs were differentially expressed between infected and uninfected mice (|fold change| ≥ 0.585, P < 0.05). These differentially expressed molecules were closely related to the JAK/STAT, mitogen-activated protein kinase, p53, and PI3K/Akt signaling pathways, cell cycle, and metabolic pathways.Conclusions: E. granulosus infection caused significant increases in the IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNF-α levels in the peripheral blood of mice, as well as significant changes in the expression levels of a variety of coding and noncoding RNAs. A further study of these trends and pathways may help clarify the pathogenesis of CE and provide new insights into the prevention and treatment of this infection.

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“…Microarray analysis is a sensitive tool to accurately investigate differentially expressed circRNAs during many disease processes [4][5][6][7][8]. It is based on hybridization and fluorescence detection and uses specific probes targeting the back-spliced junction of each circRNA.…”

Section: To the Editormentioning

confidence: 99%

Response to comments on our article (Yin YL et al., Parasit Vectors, 10.1186/s13071-021-04739-w) by Yuqing Wang and colleagues

2021

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This letter responds to comments on our article (Yin YL et al., Parasit Vectors, 10.1186/s13071-021-04739-w) by Yuqing Wang and colleagues, who wrote a letter entitled “Microarray analysis of circular RNAs in HCT-8 cells infected with Cryptosporidium parvum” and discussed statistical procedures for microarray analysis during C. parvum infection. To further confirm our data, in this letter, a common R package for analyses of differentially expressed genes, namely DESeq2, with Benjamini-Hochberg correction, was used to analyze our microarray data and identified 26 significantly differentially expressed circRNAs using adjusted P value < 0.05 and | Log2 (fold change [FC]) | ≥ 1.0, including our circRNA ciRS-7 of interest. Therefore, the protocol for selecting circRNAs of interest for further study in our article is acceptable and did not affect the subsequent scientific findings in our article.

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Expression profile of circular RNAs in cystic echinococcosis pericystic tissue

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 8 publications

References 34 publications

[Retracted] Coding and Noncoding RNA Expression Profiles of Spleen CD4+ T Lymphocytes in Mice with Echinococcosis

[Retracted] Coding and Noncoding RNA Expression Profiles of Spleen CD4+ T Lymphocytes in Mice with Echinococcosis

Coding and Noncoding RNA Expression Profiles of Spleen CD4+ T Lymphocytes in Mice With Echinococcosis

Response to comments on our article (Yin YL et al., Parasit Vectors, 10.1186/s13071-021-04739-w) by Yuqing Wang and colleagues

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