Expression profile of epithelial-mesenchymal transition markers in non–muscle-invasive urothelial carcinoma of the bladder: Correlation with intravesical recurrence following transurethral resection

Liu, Bing; Miyake, Hideaki; Nishikawa, Masatomo; Fujisawa, Masato

doi:10.1016/j.urolonc.2014.08.012

Cited by 30 publications

(31 citation statements)

References 28 publications

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“…The present study was approved by the Medical Ethics Committee of Sun Yat-sen University (Guangzhou, Guangdong, China) and written informed consent was obtained from each patient. The IHC staining assays and evaluation methods were performed as previously described (18,21). The antibody used was rabbit polyclonal ZEB1 antibody (1:200; cat.…”

Section: Clinical Tissue Samples and Immunohistochemistry (Ihc)mentioning

confidence: 99%

Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1

Mao

Wang

et al. 2018

Oncol Lett

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Abstract. The aim of the present study was to investigate the associations between vasculogenic mimicry (VM) and zinc finger E-box binding homeobox 1 (ZEB1) in bladder cancer. VM structure and ZEB1 expression were analyzed by cluster of differentiation 34/periodic acid Schiff (PAS) double staining and immunohistochemical staining in 135 specimens from patients with bladder cancer, and a further 12 specimens from normal bladder tissues. Three-dimensional (3-D) culture was used to detect VM formation in the bladder transitional cancer cell lines UM-UC-3 and J82, and the immortalized human bladder epithelium cell line SV-HUC-1 in vitro. ZEB1 expression in these cell lines was compared by reverse transcription-quantitative polymerase chain reaction and western blot assays. In addition, small interfering RNA was used to inhibit ZEB1 in UM-UC-3 and J82 cells, followed by 3-D culturing of treated cell lines. As a result, VM was observed in 31.1% of specimens from bladder cancer tissues, and cases with high ZEB1 expression accounted for 60.0% of patients with bladder cancer. In addition, ZEB1 expression was closely associated with VM (r=0.189; P<0.05), and also increased as the grade and stage of the tumor developed. In an in vitro assay, UM-UC-3 and J82 cells exhibited VM formation, however, SV-HUC-1 did not. Furthermore, VM-forming cancer cell lines UM-UC-3 and J82 exhibited higher ZEB1 expression. Notably, VM formation was inhibited following knockdown of ZEB1. In conclusion, ZEB1 may be associated with VM in bladder cancer and serve an important role in the process of VM formation. However, its detailed mechanism requires further study.

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Section: Clinical Tissue Samples and Immunohistochemistry (Ihc)mentioning

confidence: 99%

Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1

Mao

Wang

et al. 2018

Oncol Lett

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“…In support of these results, some recent studies have identified alteration in a subset of EMT markers associated with poor outcomes in conventional UCa. 29,30 Further assessment of the role of EMT in the biology of sarcomatoid UCa development and progression may be warranted in this setting.…”

Section: Emt-associated Immunomarker Expressionmentioning

confidence: 99%

Sarcomatoid Urothelial Carcinoma of the Bladder: Analysis of 28 Cases With Emphasis on Clinicopathologic Features and Markers of Epithelial-to-Mesenchymal Transition

Sanfrancesco

McKenney²,

Leivo

et al. 2016

Archives of Pathology &Amp; Laboratory Medicine

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“…Among the included articles, the protein expression level was detected using IHC in 22 studies [6,8,10,12,13,[15][16][17][18][19][20]25,[29][30][31][32][33][34][35][36][37][38], the mRNA transcriptional level was probed by RT-qPCR in 5 studies [7,9,14,22,39], and both methods were used in 1 study [10]. In terms of the NOS scale, 9 articles scored 9, 4 articles scored 8, 7 articles scored 7 and 3 articles scored 6 in the protein group; 2 articles scored 9, 2 articles scored 8 and the rest two articles scored 7 and 6, respectively, in the mRNA group ( Table S1).…”

Section: Studies Identification and Characteristics Of Eligible Studiesmentioning

confidence: 99%

The prognostic implication of slug in all tumour patients – a systematic meta‐analysis

Huang

Zhang

et al. 2016

Eur J Clin Investigation

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Expression profile of epithelial-mesenchymal transition markers in non–muscle-invasive urothelial carcinoma of the bladder: Correlation with intravesical recurrence following transurethral resection

Cited by 30 publications

References 28 publications

Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1

Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1

Sarcomatoid Urothelial Carcinoma of the Bladder: Analysis of 28 Cases With Emphasis on Clinicopathologic Features and Markers of Epithelial-to-Mesenchymal Transition

The prognostic implication of slug in all tumour patients – a systematic meta‐analysis

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