Expression Profile of Genes Regulated by Curcumin in Y79 Retinoblastoma Cells

Abstract

Curcumin, a well-known chemopreventive agent from turmeric, inhibits the expression of several oncogenes and cell proliferation genes in tumor cells. This study aims to understand the precise molecular mechanism by which curcumin exerts its effects on retinoblastoma cells, by performing whole genome microarray analysis to determine the gene expression profiles altered by curcumin treatment. Curcumin suppressed cell viability and altered the cell cycle of retinoblastoma cells. We identified 903 downregulated genes and 1,319 upregulated genes when compared with the control cells after treatment with 20 μM curcumin concentration for 48 h. These genes were grouped into respective functional categories according to their biological function. We found that curcumin regulated the expression of genes that are involved in the regulation of apoptosis, tumor suppressor, cell-cycle arrest, transcription factor, and angiogenesis. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to validate the results of genome array, and the results were consistent with the obtained data. In conclusion, treatment of curcumin affects the expression of genes involved in various cellular functions and plays an important role in tumor metastasis and apoptosis. Thus, curcumin might be an effective chemopreventive agent for retinoblastoma cancer.

Dietary curcumin inhibits atherosclerosis by affecting the expression of genes involved in leukocyte adhesion and transendothelial migration

“…Genes involved in different cellular functions have been identified, such as cellular metabolic process (GO:0044237), signal transduction (GO:0007135), cell death (GO:0008219), protein processing (GO:0016485), cell adhesion (GO:0007155), or apoptosis (GO:0006915) (Supporting Information Table S5). Some of these cellular functions have already been described as modulated at the genomic level by curcumin in different cell lines . Interestingly, in our study, classification of identified differentially expressed genes using both GO and KEGG databases, revealed that over 100 genes could be involved in cytoskeleton organization, cell junction, cell communication, or cell adhesion.…”

“…The microarray analysis showed that the expression of 1670 genes has been affected by curcumin in aorta. It has been previously reported, using transcriptome approach, that curcumin can modulate expression of genes in different cell lines, such as Y79 retinoblastoma cells [35], microglial cells [36], breast cancer cell [37], or human colon cancer cells [38], as well as in-vivo, in rat heart and liver [39,40] or mice colon [41]. To our knowledge, our study is the first to report impact of curcumin on expression of genes in aorta using genomewide approach in relation to the antiatherogenic effect of this compound.…”

Suppression of PAX6 promotes cell proliferation and inhibits apoptosis in human retinoblastoma cells

“…The Y79 cell line was obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The maintenance of these cell lines was carried out as previously described (17–19). In brief, the cells were cultured in RPMI-1640 medium (HyClone Co., Logan, UT, USA) supplemented with 10% fetal bovine serum, 100 U/l penicillin, and 100 U/l streptomycin at 37°C in a humidified atmosphere of 95% air/5% CO 2 .…”

Herbs and Spices in Aging

“…12 Additionally, curcumin has antibacterial, chemopreventive, chemotherapeutic, antinociceptive, antiparasitic and antimalarial properties 13 and it blocks HIV replication. Curcumin is non-toxic in normal cells and kills only tumor cells.…”

“…Curcumin regulates the expression of genes related to apoptosis, tumor suppression, cell-cycle arrest, transcription factor, angiogenesis and metastasis. 12 Curcumin ameliorates various chronic illnesses, linked to free radical damage, affecting the eyes, lungs, liver, kidneys and the gastrointestinal and cardiovascular systems. Curcumin modulates signaling molecules, including inflammatory molecules, transcription factors, enzymes, protein kinases, protein reductases, carrier proteins, cell-survival proteins, drug resistance proteins, adhesion molecules, growth factors, receptors, cell-cycle regulatory proteins, chemokines, DNA, RNA Normal lenses of normal rats and diabetic rats supplemented with clove.…”