Expression profile of human defensins and antimicrobial proteins in oral tissues

Dunsche, Anton; Açil, Yahya; Siebert, Reiner; Harder, Jürgen; Schröder, Jens‐M.; Jepsen, Søren

doi:10.1034/j.1600-0714.2001.300305.x

Cited by 108 publications

(93 citation statements)

References 15 publications

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“…One report observed HD-5 peptide at various sites in the human female genital tract and found high ng͞ml levels of HD-5 in vaginal wash material (48). Other studies have reported HD-5 expression in the testes and male urethra, as well as in breast milk and keratinocytes of the oral and airway mucosa (49)(50)(51)(52)(53). In some of these studies, HD-5 expression appeared to correlate with local inflammation.…”

Section: Discussionmentioning

confidence: 99%

Human α-defensins block papillomavirus infection

Buck

Day

Thompson

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

216

169

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Section: Discussionmentioning

confidence: 99%

Human α-defensins block papillomavirus infection

Buck

Day

Thompson

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

216

169

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“…Human β-defensins (HBDs or DEFB) and cathelicidin LL37 are being actively used as study models for their potent ability to kill a broad spectrum of bacteria and fungi [6,7,[9][10][11][12][13], as well as to inhibit viral infections [14,15]. HBDs are expressed mostly in epithelia [11,12,[16][17][18][19][20][21][22][23][24].…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

Capacity of human β-defensin expression in gene-transduced and cytokine-induced cells

Yin¹,

Dang²,

Zhang³

et al. 2006

Biochemical and Biophysical Research Communications

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The purpose of this study was to determine the capacity of cells transduced with human β-defensins (HBDs) to express antimicrobial peptides, since sufficient expression level is required for effective antimicrobial activity. Retroviral vector pBabeNeo and lentiviral vector SIN18cPPTRhMLV (SIN18) carrying HBDs were utilized to transduce non-HBD-expressing cells such as fibroblasts or HBD-producing oral epithelial cells. We found that HBD-3 gene transfer to fibroblasts was possible not via retrovirus but by direct vector transfection. SIN18 had high transduction efficiencies (80.9-99.9%) and transduced cells expressed higher amounts of HBD-2 than those by pBabe-Neo. Primary human gingival epithelial cells (HGECs) expressed greater amounts of HBD-2 than primary fibroblasts after lentiviral transduction. Additionally, HBD-2 secretion from transduced HGECs cells was further increased when stimulated with IL-1 or TNFα. Our data indicate that while HBD-2 expression is limited in primary fibroblasts, its expression in HGECs may be maximized by gene transduction plus cytokine induction. KeywordsHBD-2; HBD-3; Lentiviral vectors; Retroviral vectors; IL-1; TNFα; Gingival epithelial cells Natural antimicrobial peptides have been considered as an alternative option for infection control due to the increasing resistance of microbes to conventional antibiotics. Because some potent antimicrobial peptides are encoded by single genes, the possibility of artificially delivering these genes into tissues/organs to augment innate immunity against infection or creating transgenic animals to produce antimicrobial peptides has been explored [1][2][3][4][5]. Subsequently, the term antimicrobial gene therapy has emerged [6] and engineered skin NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript models have been used to test the concept of antimicrobial gene therapy both in vitro and in vivo [7,8]. Human β-defensins (HBDs or DEFB) and cathelicidin LL37 are being actively used as study models for their potent ability to kill a broad spectrum of bacteria and fungi [6,7,[9][10][11][12][13], as well as to inhibit viral infections [14,15]. HBDs are expressed mostly in epithelia [11,12,[16][17][18][19][20][21][22][23][24]. Besides the cloned, sequenced, and well-characterized HBD-1, -2, -3, and -4, there appears to be at least 28 more HBDs existing based on the genomic surveys using computational search strategy [25][26][27]. Fourteen HBDs or DEFB gene transcripts (DEFB-1, -4, and DEFB-103 to -114) were investigated using reverse transcriptionpolymerase chain reaction (RT-PCR) to detect their expression in gingival keratinocytes and a selected few have been classified as constitutive or inducible [27].HBDs are considered to play an important role in epithelial defense mechanism and the regulation of their expression in diseased conditions is being actively investigated. Bacteria and cytokines IL-1 and TNFα upregulate HBD-2 expression in lung and skin epithelial cells [10,28] and induction of HBDs by these stimuli is...

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“…The importance of the protective role played by ␤-defensins against pathogenic microorganisms in oral epithelia has been discussed by several authors (5,15,18). Moreover, a recent observation by Putsep et al on patients suffering from morbus Kostmann (24) highlights the role of antimicrobial peptides in periodontal disease.…”

mentioning

confidence: 99%

Novel Hairpin-Shaped Primer Assay To Study the Association of the −44 Single-Nucleotide Polymorphism of theDEFB1Gene with Early-Onset Periodontal Disease

Boniotto

Hazbón

Jordan

et al. 2004

Clin Vaccine Immunol

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A powerful, cost-effective new method for studying single-nucleotide polymorphisms (SNPs) is described. This method is based on the use of hairpin-shaped primers (HP), which give a sensitive and specific PCR amplification of each specific allele, without the use of costly fluorophore-labeled probes and any post-PCR manipulation. The amplification is monitored in real-time using SYBR Green I dye and takes only 2 h to yield results. The HP assay has a simple design and utilizes a conventional real-time PCR apparatus. The ؊44 C3G transversion in the DEFB1 gene (which encodes human ␤-defensin 1) has been previously associated with Candida carriage in oral epithelia. In this study, we analyzed the association between early-onset periodontal disease (EOP) and the ؊44 SNP. We used an HP assay to study the distribution of the ؊44 SNP in 264 human DNAs obtained from two cohorts of EOP patients and healthy controls from different ethnic backgrounds. The results indicate that the ؊44 SNP has a similar distribution between EOP and healthy patients, suggesting that it is not associated with the disease.

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Expression profile of human defensins and antimicrobial proteins in oral tissues

Cited by 108 publications

References 15 publications

Human α-defensins block papillomavirus infection

Human α-defensins block papillomavirus infection

Capacity of human β-defensin expression in gene-transduced and cytokine-induced cells

Novel Hairpin-Shaped Primer Assay To Study the Association of the −44 Single-Nucleotide Polymorphism of theDEFB1Gene with Early-Onset Periodontal Disease

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