Expression profile of immune response genes during acute myopathy induced by chikungunya virus in a mouse model

Patil, Dilip R.; Hundekar, Supriya; Arankalle, Vidya A.

doi:10.1016/j.micinf.2011.12.008

Cited by 31 publications

(32 citation statements)

References 33 publications

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“…CHIKV is known to induce cellular inflammatory responses and the level of certain cytokines in CHIKV-infected patient serum has been shown to correlate with the symptomatic phase of infection. We therefore wanted to study the expression of selected cytokine genes that were previously shown to be activated during symptomatic phase of CHIKV infection and involved in the genesis of inflammatory host responses, including rheumatoid arthritis (RA) [30]. 293T cells were infected with either CHIKV T-SBY or V-181 at a MOI of 10.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Chalaem

Chusri

Fernandez

et al. 2016

Virol J

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BackgroundChikungunya virus (CHIKV) is a prevalent mosquito-borne pathogen that is emerging in many parts of the globe causing significant human morbidity. Here, we report the isolation and characterization of an infectious CHIKV from banked serum specimens of suspected patients from the 2009 epidemic in Thailand.MethodsStandard plaque assay was used for CHIKV isolation from the banked serum specimens. Isolated CHIKV was identified base on E1 structural gene sequence. Growth kinetic, infectivity, cell viability and cytokine gene expression throughout CHIKV infection in a permissive cell line, 293T cells, was performed using several approaches, including standard plaque assay, immunofluorescence assay, classical MTT assay, and quantitative real-time PCR. Two tailed Student’s t test was used for evaluation statistically significance between the mean values of the groups.ResultsBased on the E1 structural gene sequence and phylogenetic analysis, we identified the virus as the CHIK/SBY8/10 isolate from Indonesia. Assessment of the growth kinetics, cytopathic effects as well as its ability to induce cellular immune responses suggested that the currently isolated CHIK/SBY8/10 virus is relatively more virulent than a known CHIKV vaccine strain, which also induces more dramatic proinflammatory responses.ConclusionsOur studies further add to the infectivity of a less-studied yet infectious CHIKV isolate as well as underscored the importance and utility of 293T cells as an excellent cell culture model for studying viral growth, CHIKV-induced inflammatory cellular responses and cell death. Together, these studies provide novel information on the CHIKV biology, infectivity and virus-cell interaction, which would help develop novel interventions against the infection.

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Section: Resultsmentioning

confidence: 99%

Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Chalaem

Chusri

Fernandez

et al. 2016

Virol J

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“…Several studies on alphaviruses-induced arthritis using animal models or patients samples support that the macrophage production of inflammatory mediators, such as TNF, in damaged tissue, contributes to the establishment of the disease (Way et al 2002, Lidbury et al 2008, Gardner et al 2010, Chopra and Venugopalan 2011, Patil et al 2012, Dhanwani et al 2014. In agreement to these observations, we showed here that MAYV and SINV replication in RAW and primary macrophages induces TNF release.…”

Section: Discussionmentioning

confidence: 99%

Macrophages as target cells for Mayaro virus infection: involvement of reactive oxygen species in the inflammatory response during virus replication

Cavalheiro

Costa

Campos

et al. 2016

An. Acad. Bras. Ciênc.

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Alphaviruses among the viruses that cause arthritis, consisting in a public health problem worldwide by causing localized outbreaks, as well as large epidemics in humans. Interestingly, while the Old World alphaviruses are arthritogenic, the New World alphaviruses cause encephalitis. One exception is Mayaro virus (MAYV), which circulates exclusively in South America but causes arthralgia and is phylogenetically related to the Old World alphaviruses. Although MAYV-induced arthritis in humans is well documented, the molecular and cellular factors that contribute to its pathogenesis are completely unknown. In this study, we demonstrated for the first time that macrophages, key players in arthritis development, are target cells for MAYV infection, which leads to cell death through apoptosis. We showed that MAYV replication in macrophage induced the expression of TNF, a cytokine that would contribute to pathogenesis of MAYV fever, since TNF promotes an inflammatory profile characteristic of arthritis. We also found a significant increase in the production of reactive oxygen species (ROS) at early times of infection, which coincides with the peak of virus replication and precedes TNF secretion. Treatment of the cells with antioxidant agents just after infection completely abolished TNF secretion, indicating an involvement of ROS in inflammation induced during MAYV infection.

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“…In the 2005 outbreak, Sri Lanka reported 62.5% of patients affected 37 . Abnormalities basically consist of sinus bradycardia, T inversion, depression of ST segment in precordial leads and avF, AV blocks, (Mobitz type I second degree has been mentioned) bundle branch blocks and rarely, atrial fibrillation 33, 34, 37, 38 . All were reported as supposedly transient 39 .…”

Section: Resultsmentioning

confidence: 99%

Cardiovascular involvement and manifestations of systemic Chikungunya virus infection: A systematic review

et al. 2017

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Background: In the last three years, chikungunya virus disease has been spreading, affecting particularly the Americas, producing more than two million cases. In this setting, not only new disease-related epidemiological patterns have been found, but also new clinical findings have been reported by different research groups. These include findings on the cardiovascular system, including clinical, electrocardiographic and echocardiographic alterations. Methods: We performed a systematic review looking for reports about cardiovascular compromise during chikungunya disease. Cardiac compromise is not so common in isolated episodes; but countries where chikungunya virus is an epidemic should be well informed about this condition. We used 6 bibliographical databases as resources: Medline/Pubmed, Embase, ScienceDirect, ClinicalKey, Ovid and SciELO. Dengue reports on cardiovascular affectation were included as well, to compare both arbovirus’ organic affectations. Articles that delved mainly into the rheumatic articular and cutaneous complications were not considered, as they were not in line with the purpose of this study. The type of articles included were reviews, meta-analyses, case-controls, cohort studies, case reports and case series. Results: Originally based on 737 articles, our reviewed selected 40 articles with 54.2% at least mentioning CHIKV cardiovascular compromise within the systemic affectation. Cardiovascular manifestations can be considered common and have been reported in France, India, Sri Lanka, Malaysia, Colombia, Venezuela and USA, including mainly, but no limited to: hypotension, shock and circulatory collapse, Raynaud phenomenon, arrhythmias, murmurs, myocarditis, dilated cardiomyopathy, congestive insufficiency, heart failure and altered function profile (Troponins, CPK). Conclusions: Physicians should be encouraged to keep divulgating reports on the cardiovascular involvement of chikungunya virus disease, to raise awareness and ultimately encourage suitable diagnosis and intervention worldwide.

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Expression profile of immune response genes during acute myopathy induced by chikungunya virus in a mouse model

Cited by 31 publications

References 33 publications

Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Macrophages as target cells for Mayaro virus infection: involvement of reactive oxygen species in the inflammatory response during virus replication

Cardiovascular involvement and manifestations of systemic Chikungunya virus infection: A systematic review

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