Expression profile of tsRNAs in white adipose tissue of vitamin D deficiency young male mice with or without obesity

Abstract

Background The expression of tsRNA in white adipose tissue (WAT) of VD deficiency male mice with obesity has not been reported. Methods The healthy male C57BL/6J mice aged 4–6 weeks were divided into 4 groups according to the VD3 and fat energy supplement in daily diets. Results The qPCR verification further demonstrated that 1 tsRNAs (tRF5-20-HisGTG-3, all P < 0.05) were significantly up-regulated and 1 tsRNA (mt-tRF3a-ProTGG, all P < 0.05) was significantly down-regulated not only in HFVDD vs HFVDS, but aslo in HFVDD vs ConVDS. 1 tsRNAs (tRF5-22-CysGCA-27, P < 0.05) were significantly up-regulated and 3 tsRNA (mt-5'tiRNA-32-SerTGA, mt-5'tiRNA-33-SerTGA and mt-5'tiRNA-33-AlaTGC, all P < 0.05) was significantly down-regulated only in HFVDD vs ConVDS. Enrichment analysis of the qPCR verified DE tsRNAs showed that the 3 up-regulated tsRNAs seemed to be associated with FoxO signaling pathway, GnRH secretion, 2 − Oxocarboxylic acid metabolism, Autophagy – animal, Glucagon signaling pathway, AGE − RAGE signaling pathway in diabetic complications, Insulin signaling pathway, Apelin signaling pathway, Alzheimer disease, Pathways of neurodegeneration − multiple diseases, while 4 down-regulated tsRNA seemed to be associated with cell communication, primary metabolic process, metabolic process, response to stimulus, multicellular organismal process, cellular metabolic process, regulation of cellular process, regulation of biological process, and biological regulation. Conclusions The tsRNAs were differentially expressed in VD deficiency with obesity, especially tRF5-20-HisGTG-3, tRF5-22-CysGCA-27, tRF3a-GlyGCC-1, mt-5'tiRNA-33-AlaTGC, mt-5'tiRNA-33-SerTGA, mt-5'tiRNA-32-SerTGA and mt-tRF3a-ProTGG. These tsRNAs seemed to be associated with FoxO signaling pathway, GnRH secretion, 2 − oxocarboxylic acid metabolism, autophagy, glucagon and insulin signaling pathway, pathways of neurodegeneration − multiple diseases, metabolic process and biological regulation.