Expression profile, subcellular localization and signaling pathway analysis of fish-specific TLR25 in Nile tilapia (Oreochromis niloticus)

Lee, Po‐Tsang; Ho, Thi Hang; Nguyen, Bao Trung; Lin, Yulin; Chiu, Po-Yu

doi:10.1016/j.fsi.2020.05.028

Cited by 16 publications

(8 citation statements)

References 70 publications

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“…Bacterial infection (or lysates) causes the delivery of ligands, such as LTA, peptidoglycan, flagellin, single-stranded (ss) RNA, double-stranded (ds) RNA and CpG DNA, to the host, which activates multiple TLRs, such as TLR1-3, TLR5, TLR7-9, TLR21, and teleost-specific TLR19 ( 51 ) and TLR22 ( 52 ). Additionally, our recent research revealed that the transcript levels of Nile tilapia TLR18 ( 27 ) and TLR25 ( 28 ) were elevated in the head kidney during bacterial infection and that these two fish-specific TLRs can physically interact with both MyD88 and TRIF, adding the possibility that they may recognize (unknown) bacteria-derived ligands and launch the production of proinflammatory cytokines and type I IFNs after activation.…”

Section: Discussionmentioning

confidence: 99%

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Sterile alpha and TIR motif-containing protein 1 is a negative regulator in the anti-bacterial immune responses in nile tilapia (Oreochromis niloticus)

Trung

Nguyen²,

Hsueh³

et al. 2022

Front. Immunol.

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Nile tilapia (Oreochromis niloticus) is one of the most important food fish in the world. However, the farming industry has encountered significant challenges, such as pathogen infections. Toll-like receptors (TLRs) play an essential role in the initiation of the innate immune system against pathogens. Sterile alpha and TIR motif-containing protein 1 (SARM1) is one of the most evolutionarily conserved TLR adaptors, and its orthologs are present in various species from worms to humans. SARM1 plays an important role in negatively regulating TIR domain-containing adaptor proteins inducing IFNβ (TRIF)-dependent TLR signaling in mammals, but its immune function remains poorly understood in fish. In this study, O. niloticus SARM1 (OnSARM1) was cloned and its evolutionary status was verified using bioinformatic analyses. mRNA expression of OnSARM1 was found at a higher level in the trunk kidney and muscle in healthy fish. The examination of its subcellular location showed that the OnSARM1 was detected only in the cytoplasm of THK cells, and colocalized with OnMyD88, OnTRIF and OnTRIF in small speckle-like condensed granules. The transcript levels of OnMyD88, OnTIRAP, OnTRIF, and downstream effectors, including interleukin (IL)-1β, IL-8, IL-12b and type I interferon (IFN)d2.13, were regulated conversely to the expression of OnSARM1 in the head kidney from Aeromonas hydrophila and Streptococcus agalactiae infected fish. Moreover, the treatment of THK cells with lysates from A. hydrophila and S. agalactiae enhanced the activity of the NF-κB promoter, but the effects were inhibited in the OnSARM1 overexpressed THK cells. Overexpression of OnSARM1 alone did not activate the NF-κB-luciferase reporter, but it suppressed OnMyD88- and OnTIRAP-mediated NF-κB promoter activity. Additionally, OnSARM1 inhibited the mRNA expression of proinflammatory cytokines and hepcidin in A. hydrophila lysate stimulated THK cells. Taken together, these findings suggest that OnSARM1 serves as a negative regulator by inhibiting NF-κB activity, thereby influencing the transcript level of proinflammatory cytokines and antimicrobial peptides in the antibacterial responses.

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Section: Discussionmentioning

confidence: 99%

“…Western blotting was conducted according to procedures described in previous research ( 28 ). HEK 293 cells were seeded in 24-well plates at a density of 1 × 10 5 cells per well the day before transfection.…”

Section: Methodsmentioning

confidence: 99%

Sterile alpha and TIR motif-containing protein 1 is a negative regulator in the anti-bacterial immune responses in nile tilapia (Oreochromis niloticus)

Trung

Nguyen²,

Hsueh³

et al. 2022

Front. Immunol.

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“…To date, 18 different TLRs have been identified in fish species. In teleost fish, TLR25 is an identified fish-specific member of the TLR1 subfamily, and is involved in activating NF-κB and type I IFNs signaling pathways and inducing the expression of proinflammatory cytokines such as TNF-α, IL1β and IL8 ( 32 , 33 ). Previous studies have suggested that TLR2 and TLR4 are receptors for histones, and required for responses to all individual histones ( 34 , 35 ).…”

Section: Discussionmentioning

confidence: 99%

Immunoprotective Effects of Two Histone H2A Variants in the Grass Carp Against Flavobacterium columnare Infection

et al. 2022

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In teleost fish, the nucleotide polymorphisms of histone H2A significantly affect the resistance or susceptibility of zebrafish to Edwardsiella piscicida infection. Whether histone H2A variants can enhance the resistance of grass carp to Flavobacterium columnare infection remains unclear. Here, the effects of 7 previously obtained variants (gcH2A-1~gcH2A-7) and 5 novel histone H2A variants (gcH2A-11, gcH2A-13~gcH2A-16) in response to F. columnare infection were investigated. It was found that these histone H2A variants could be divided into type I and II. Among them, 5 histone H2A variants had no any effects on the F. columnare infection, however 7 histone H2A variants had antibacterial activity against F. columnare infection. The gcH2A-4 and gcH2A-11, whose antibacterial activity was the strongest in type I and II histone H2A variants respectively, were picked out for yeast expression. Transcriptome data for the samples from the intestines of grass carp immunized with the engineered Saccharomyces cerevisiae expressing PYD1, gcH2A-4 or gcH2A-11 revealed that 5 and 12 immune-related signaling pathways were significantly enriched by gcH2A-4 or gcH2A-11, respectively. For the engineered S. cerevisiae expressing gcH2A-4, NOD-like receptor and Toll-like receptor signaling pathways were enriched for up-regulated DEGs. Besides NOD-like receptor and Toll-like receptor signaling pathways, the engineered S. cerevisiae expressing gcH2A-11 also activated Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway and C-type lectin receptor signaling pathway. Furthermore, grass carp were immunized with the engineered S. cerevisiae expressing PYD1, gcH2A-4 or gcH2A-11 for 1 month and challenged with F. columnare. These grass carp immunized with gcH2A-4 or gcH2A-11 showed lower mortality and fewer numbers of F. columnare than did the control group. All these results suggest that gcH2A-4 and gcH2A-11 play important roles in evoking the innate immune responses and enhancing disease resistance of grass carp against F. columnare infection.

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“…Kureishi et al (1997) research has demonstrated that Rho‐kinase directly modulates smooth muscle contraction through myosin light chain phosphorylation, independently of the Ca 2+ ‐calmodulin‐dependent myosin light chain kinase pathway. In multicellular organisms, phagocytosis plays a role in maintaining cellular homeostasis by removing dead cells, microbes and cellular or foreign debris (Lee et al, 2020; Lee et al, 2020). Bandola‐Simon and Roche's (2019) research has shown that MLC2 in hepatopancreas tissue may resist the infection of Vibrio anguillae by triggering actin rearrangement and forming phagosomes.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals immune‐related differentially expressed genes in the hepatopancreas and gills of Penaeus vannamei after Vibrio anguillarum infection

Song

Soomro

et al. 2021

Aquaculture Research

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Penaeus vannamei is one of the commercially important aquaculture species in the world but sensitive to Vibrio anguillarum infection. To understand the immune response of P. vannamei against V. anguillarum infection, the transcriptomes of hepatopancreas and gills of P. vannamei were analysed before and after V. anguillarum infection. Total 6213 differentially expressed genes (DEGs) were obtained from the hepatopancreas, and 3780 were obtained from the gills. The DEGs of interest were screened to construct a heat map. The reliability and accuracy of the transcriptome data were verified using RT‐qPCR. HSP70 and ARF6 were found highly expressed in both hepatopancreas and gills, while MLC2s encoded by two different gene sequences were found to be expressed in hepatopancreas and gills. Six immune‐related DEGs were identified from the protein–protein interaction (PPI) network including the HSP70, ARF6 and MLC2s. The sub‐network of PPI was constructed to explore the mode of interaction of immune‐related DEGs with their neighbouring proteins. Based on the result of PPI network, we infer that phosphorylated MLC2‐1 conducts signal transduction to induce leukocyte transendothelial migration in P. vannamei gills tissue while MLC2‐2 plays a role in immunity of V. anguillarum infection not only through activating signal transduction to mediate immune responses but also through the formation of phagosomes in hepatopancreas tissue. HSP70 is associated with the transporting exogenous cargos through presenting antigenic peptides in the process of V. anguillarum infection in P. vannamei. ARF6 interacts with Rab proteins to regulate phagolysosome formation by regulating GTPase activity in P. vannamei gills tissue. In hepatopancreas tissue, ARF6 plays a role in immunity by interacting with cytohesin. This study will contribute to further exploring the immune mechanism of P. vannamei to V. anguillarum infection.

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Expression profile, subcellular localization and signaling pathway analysis of fish-specific TLR25 in Nile tilapia (Oreochromis niloticus)

Cited by 16 publications

References 70 publications

Sterile alpha and TIR motif-containing protein 1 is a negative regulator in the anti-bacterial immune responses in nile tilapia (Oreochromis niloticus)

Sterile alpha and TIR motif-containing protein 1 is a negative regulator in the anti-bacterial immune responses in nile tilapia (Oreochromis niloticus)

Immunoprotective Effects of Two Histone H2A Variants in the Grass Carp Against Flavobacterium columnare Infection

Transcriptome analysis reveals immune‐related differentially expressed genes in the hepatopancreas and gills of Penaeus vannamei after Vibrio anguillarum infection

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