Expression profile, transcriptional and post‐transcriptional regulation of genes involved in hydrogen sulphide metabolism connecting the balance between development and stress adaptation in plants: a data‐mining bioinformatics approach

Abstract

Recent research focused on novel aspects of sulphur and sulphur-containing molecules in fundamental plant processes has highlighted the importance of these compounds. Currently, the focus has shifted to the efficacy of hydrogen sulphide (H 2 S) as signalling compounds that regulate different development and stress mitigation in plants.• Accordingly, we used an in silico approach to study the differential expression patterns of H 2 S metabolic genes at different growth/development stages and their tissue-specific expression patterns under a range of abiotic stresses. Moreover, to understand the multilevel regulation of genes involved in H 2 S metabolism, we performed computationbased promoter analysis, alternative splice variant analysis, prediction of putative miRNA targets and co-expression network analysis.• Gene expression analysis suggests that H 2 S biosynthesis is highly influenced by developmental and stress stimuli. The functional annotation of promoter structures reveales a wide range of plant hormone and stress responsive cis-regulatory elements (CREs) that regulate H 2 S metabolism. Co-expression analysis suggested that genes involved in H 2 S metabolism are also associated with different metabolic processes.• In this data-mining study, the primary focus was to understand the genetic architecture governing pathways of H 2 S metabolism in different cell compartments under various developmental and stress signalling cascades. The present study will help to understand the genetic architecture of H 2 S metabolism via cysteine metabolism and the functional roles of these genes in development and stress tolerance mechanisms.

Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)

“…This expression leads to biotic and abiotic stresses ( Kurepa et al., 2009 ). The presence of light-responsive elements indicates that they are required for light-dependent transcriptional regulation ( Hiratsuka and Chua, 1997 ; Mondal et al., 2022 ). Similarly, the presence of endosperm elements is expressed during developmental stages.…”

From Classical to Modern Computational Approaches to Identify Key Genetic Regulatory Components in Plant Biology

“…The most straightforward application of co-expression network analysis is the study of metabolic pathways and their regulation [104][105][106][107], including plant stress responses [108][109][110] and, more importantly, the identification of potential candidates for the biotechnological improvement of crops [51]. This is of special relevance, as it makes it possible to directly transfer all knowledge gained in model species over the years to species of agronomic interest, such as soybean [108].…”

Emerging role of hydrogen sulphide as a signalling molecule in plant biology

“…This special issue helps to elucidate the interactions of H 2 S with other signalling molecules and phytohormones in plants under different environmental stresses. Mondal et al (2022) explored the differential expression levels of H 2 S metabolic genes at different growth/development stages and their tissuespecific expression patterns under environmental stress. Analysis of gene expression reveals that H 2 S biosynthesis is triggered by developmental and stress stimuli.…”

“…Mondal et al . (2022) explored the differential expression levels of H 2 S metabolic genes at different growth/development stages and their tissue‐specific expression patterns under environmental stress. Analysis of gene expression reveals that H 2 S biosynthesis is triggered by developmental and stress stimuli.…”