Expression Profiles and Possible Roles of Galectins in the Corpus Luteum

Nio‐Kobayashi, Junko; Hashiba, Kazuhisa; Sano, Masahiro; Okuda, Kiyoshi; Duncan, W. Colin; Iwanaga, Toshihiko

doi:10.4052/tigg.1416.1e

Cited by 3 publications

(4 citation statements)

References 27 publications

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“…LGALS1 gene encodes Gal1, one of 15 members of a family of β-galactoside-binding proteins called galectins, which are described to function as homodimeric proteins consisting of 14.5 kDa subunits. Gal1 expression is regulated via the luteinizing hormone/choriogonadotropin receptor (LHCGR), a common receptor for LH and hCG [ 19 ]. Interestingly, hCG stimulation in hGL5 cells stimulated intracellular accumulation of cAMP in hGL5 cells [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

Tyrosine Kinase Inhibitor Profiling Using Multiple Forskolin-Responsive Reporter Cells

Kasahara,

Tamamura,

Hiyama

et al. 2023

IJMS

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We have developed a highly sensitive promoter trap vector system using transposons to generate reporter cells with high efficiency. Using an EGFP/luciferase reporter cell clone responsive to forskolin, which is thought to activate adenylate cyclase, isolated from human chronic myelogenous leukemia cell line K562, we found several compounds unexpectedly caused reporter responses. These included tyrosine kinase inhibitors such as dasatinib and cerdulatinib, which were seemingly unrelated to the forskolin-reactive pathway. To investigate whether any other clones of forskolin-responsive cells would show the same response, nine additional forskolin-responsive clones, each with a unique integration site, were generated and quantitatively evaluated by luciferase assay. The results showed that each clone represented different response patterns to the reactive compounds. Also, it became clear that each of the reactive compounds could be profiled as a unique pattern by the 10 reporter clones. When other TKIs, mainly bcr-abl inhibitors, were evaluated using a more focused set of five reporter clones, they also showed unique profiling. Among them, dasatinib and bosutinib, and imatinib and bafetinib showed homologous profiling. The tyrosine kinase inhibitors mentioned above are approved as anticancer agents, and the system could be used for similarity evaluation, efficacy prediction, etc., in the development of new anticancer agents.

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Section: Resultsmentioning

confidence: 99%

Tyrosine Kinase Inhibitor Profiling Using Multiple Forskolin-Responsive Reporter Cells

Kasahara,

Tamamura,

Hiyama

et al. 2023

IJMS

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“…We previously reported that the corpus luteum, which produces progesterone for the establishment and maintenance of pregnancy, stage-specifically produces galectin-1 and -3 in mice, cows, and women, suggesting their involvement in the regulation of luteal function (17). Changes in galectin expression during the estrous cycle are also observed in other parts of the female reproductive tract, including the oviduct, uterus, and vagina, indicating that ovarian steroids regulate galectin expression in these organs (6).…”

Section: Galectins In the Reproductive And Endocrine Systemsmentioning

confidence: 99%

“…Galectin-1 expression in luteal cells is induced by luteotrophic LH, hCG, and PGE, while the mechanisms regulating The cellular expression of galectins correlates with changes in the glycan structure. We previously reported that α2,6-si- alylation on the terminal galactose on glycoconjugates, which inhibits galectin-1 binding, negatively correlated with the expression of galectin-1 in luteal cells (17). The differential expression of α2,6-linked sialic acid and galectin-1 has also been observed in cancer cells, and is related to sensitivity to anti-VEGF treatments to inhibit angiogenesis in tumors (33).…”

Section: G What Regulates Galectin Expression?mentioning

confidence: 99%

“…In the ovary, we found that the molecules regulating luteal function, namely, prolactin (PRL), prostaglandin E 2 (PGE) and PGF 2α (PGF), luteinizing hormone (LH), and human chorionic gonadotrophin (hCG), affected the galectin expression in luteal cells (17). Galectin-1 expression in luteal cells is induced by luteotrophic LH, hCG, and PGE, while the mechanisms regulating The cellular expression of galectins correlates with changes in the glycan structure.…”

Section: G What Regulates Galectin Expression?mentioning

confidence: 99%

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Histological Mapping and Subtype-Specific Functions of Galectins in Health and Disease

Nio‐Kobayashi

2018

Trends in Glycoscience and Glycotechnology

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Galectins, β-galactoside-binding lectins, consist of 15 members and are broadly distributed in the mammalian body in organand cell-specific manners. This minireview summarizes current knowledge on the cellular localization of galectin subtypes in various organs including the digestive tract, lymphatic system, respiratory system, urinary system, and reproductive system. We also summarize the specific localization of galectins in the epithelium with a focus on the characteristic morphologies of epithelia. In addition, we discuss the functions of galectins in the reproductive and endocrine systems, pathogenic angiogenesis, and the regulation of stem cells. The regulatory mechanism of galectin expression is also discussed based on findings obtained from luteal cells. Various glycoconjugate ligands for galectins have been identified, and notably the ligands for galectins differ in each galectin-expressing cell. The physiological and pathological states of cells affect the expression of galectin and glycoconjugate ligands, and the extracellular environment, such as the concentration of growth factors, nutrients, and oxygen also controls the expression levels. Future studies to identify the cellular and intracellular localization of galectins using histological analyses will provide a better understanding of the potential functions of galectins in healthy and disease states.

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Possible Contribution of Alpha2,6-Sialylation to Luteolysis in Cows by Inhibiting the Luteotropic Effects of Galectin-1

Hashiba

Nio‐Kobayashi

Sano

et al. 2016

Biology of Reproduction

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The corpus luteum (CL) is essential for establishing pregnancy. If pregnancy does not occur during the estrous cycle, luteolysis is induced by prostaglandin (PG) F2alpha secreted from the uterus. Galectin-1, a beta-galactose-binding protein, is expressed in the functional CL of cows and increases the viability of bovine luteal steroidogenic cells (LSCs) by modifying the functions of membrane glycoproteins. The binding of galectin-1 to glycoproteins is blocked by alpha2,6-sialylation of the terminal galactose residues of glycoconjugates, which is catalyzed by a sialyltransferase (ST6Gal-I). However, the physiological role of alpha2,6-sialic acid in bovine CL is unclear. The level of alpha2,6-sialylation of the bovine CL was higher during the regressed-luteal stage than in other luteal stages. Lectin histochemistry revealed that alpha2,6-sialylated glycoconjugates were localized to luteal endothelial cells throughout the estrous cycle. In addition, alpha2,6-sialylated glycoconjugates concentrated to the membrane of LSCs during the regressed-luteal stage. PGF2alpha treatment for 72 h enhanced the expression of ST6Gal-I mRNA and the level of alpha2,6-sialylated glycoproteins in mid-LSCs. The level of alpha2,6-sialylated glycoproteins of late-stage LSCs (Days 15-17 after ovulation) was higher than that of mid-stage LSCs (Days 8-12 after ovulation), and galectin-1 increased the viability of mid-LSCs but not that of late-stage LSCs. Furthermore, galectin-1 increased the viability of late-LSCs when alpha2,6-sialic acid residues were removed by neuraminidase. The overall findings suggest that alpha2,6-sialylation stimulated by PGF2alpha contributes to luteolysis by inhibiting the luteotropic effects of galectin-1 in bovine CL.

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Expression Profiles and Possible Roles of Galectins in the Corpus Luteum

Cited by 3 publications

References 27 publications

Tyrosine Kinase Inhibitor Profiling Using Multiple Forskolin-Responsive Reporter Cells

Tyrosine Kinase Inhibitor Profiling Using Multiple Forskolin-Responsive Reporter Cells

Histological Mapping and Subtype-Specific Functions of Galectins in Health and Disease

Possible Contribution of Alpha2,6-Sialylation to Luteolysis in Cows by Inhibiting the Luteotropic Effects of Galectin-1

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