Expression profiles of genes related to carbohydrate metabolism provide new insights into carbohydrate accumulation in seeds and seedlings of Ricinus communis in response to temperature

Ribeiro, Paulo R.; Ligterink, Wilco; Hilhorst, Henk W. M.

doi:10.1016/j.plaphy.2015.07.023

Cited by 15 publications

(3 citation statements)

References 49 publications

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“…We consider that gluconeogenesis in Ricinus roots was suppressed under salt stress, resulting in a decrease of glucose content (Ribeiro et al . 2015). The changes of these sugars are in accordance with changes in enzymes associated with sucrose metabolism, where activity of SPS did not change significantly (Fig.…”

Section: Resultsmentioning

confidence: 99%

Response of sugar metabolism in the cotyledons and roots of Ricinus communis subjected to salt stress

Chu

Yao

et al. 2022

Plant Biol J

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Ricinus communis is an important oilseed crop worldwide and is also considered one of the best potential plants for salt-affected soil improvement in northeast China. However, little is known about photosynthesis and carbohydrate metabolism in this plant, nor the distribution of carbohydrates in cotyledons and roots under salinity stress.• In the present study, seedling growth, gas exchange parameters (P N , E, g s and C i ), carbohydrate (fructose, sucrose, glucose, soluble sugar and starch) metabolism and related enzymes and genes were measured in Ricinus plants.• Under salt stress, P N of cotyledons decreased significantly (P < 0.05), resulting in weak photosynthetic capacity. Furthermore, salt stress increased sucrose and glucose content in cotyledons, but decreased soluble sugar and starch content. However, sucrose increased and starch decreased in roots. This may be correlated with the increasing sugar metabolism under salinity, including notable changes in sugar-related enzyme activities (SPS, SuSy, α-amylase and β-amylase) and gene expression of RcINV, RcSUS, RcAmY, RcBAM and RcGBE1.• The results suggest that salinity reduces photosynthesis of cotyledons, alters carbohydrate allocation between cotyledons and roots and also promotes starch utilization in cotyledons and starch biosynthesis in roots, leading to a functional imbalance between cotyledons and roots. Together, these findings provide insights into the crucial role of sugar metabolism in improving salt-tolerance of Ricinus during the early seedling growth stage.

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Section: Resultsmentioning

confidence: 99%

Response of sugar metabolism in the cotyledons and roots of Ricinus communis subjected to salt stress

Chu

Yao

et al. 2022

Plant Biol J

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“…Normalized data were uploaded in MetaboAnalyst 2.0; a web-based analytical pipeline for high-throughput studies as described previously (Bittencourt et al, 2015;Ribeiro et al, 2015a;Xia et al, 2012). Before data analysis, a data integrity check was performed to ensure that all the necessary information was present.…”

Section: Principal Component Analysis (Pca)mentioning

confidence: 99%

“…Temperature is one of the main environmental factors influencing seed germination and plant growth (Ribeiro et al, 2014(Ribeiro et al, , 2015aRibeiro et al, 2015b, d). Temperatures above the optimum are perceived as heat stress (HS) by all living organisms and this optimum temperature may vary between species (Chamorro et al, 2017;Cochrane, 2017).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of Ricinus communis L. provides new insights underlying the mechanisms towards thermotolerance during seed imbibition and germination

Ribeiro

Willems

Silva

et al. 2018

Industrial Crops and Products

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A B S T R A C TRicinus communis seeds germinate to a high percentage and faster at 35°C than at lower temperatures, but with compromised seedling establishment and survival. However, seedlings are able to cope with high temperatures at later stages of seedling establishment if germination occurred at lower temperatures. The identification of this thermo-sensitive window during seed germination suggests that temperature disturb crucial mechanisms that support seedling establishment. We studied the molecular mechanisms that could explain this thermo-sensitive window with a genomics approach using microarray analysis to determine transcriptome changes during seed germination at 20, 25 and 35°C. Although temperature had a strong effect on the R. communis transcriptome, most of these differences occurred between 6 h of imbibition and the commencement of germination, i.e. radicle protrusion, which coincided with the identified thermo-sensitive window. We identified several responsive genes that might be involved in the thermotolerance of R. communis. Temperature had a major effect on genes involved in energy generating pathways, such as the Calvin-Benson-Bassham cycle, gluconeogenesis, and starch-and triacylglycerol degradation. Transcripts of ATP binding proteins, DNA binding proteins, RNA binding proteins, DNA-directed RNA polymerases, heat shock factor proteins, multiprotein-bridging factor proteins, and zinc finger proteins were also affected by temperature suggesting that transcriptional reprogramming mechanisms were disturbed. Among the downregulated transcripts, three were shared by all three stages: one oxidationrelated zinc finger 2, one F-box and wd40 domain protein, and one DNA binding protein/ MYB-like transcription factor. Among the upregulated transcripts, nine were shared by all three stages: one BET1P/SFT1P-like protein 14BB, one low-molecular-weight cysteine-rich protein LCR78, one WD-repeat protein, one GAST1 protein, one adenylate kinase 1/P-loop containing nucleoside triphosphate hydrolases superfamily protein, and four conserved hypothetical proteins. These genes constitute good candidate genes for further characterization of temperature-responsive molecular mechanisms in R. communis, which in turn will provide necessary tools for the exploitation of R. communis by small family farmers under the typical harsh conditions of semiarid regions worldwide.

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Castor Bean Metabolomics: Current Knowledge and Perspectives Toward Understanding of Plant Plasticity Under Stress Condition

Ribeiro

Canuto

Brito

et al. 2018

Compendium of Plant Genomes

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Expression profiles of genes related to carbohydrate metabolism provide new insights into carbohydrate accumulation in seeds and seedlings of Ricinus communis in response to temperature

Cited by 15 publications

References 49 publications

Response of sugar metabolism in the cotyledons and roots of Ricinus communis subjected to salt stress

Response of sugar metabolism in the cotyledons and roots of Ricinus communis subjected to salt stress

Transcriptome profiling of Ricinus communis L. provides new insights underlying the mechanisms towards thermotolerance during seed imbibition and germination

Castor Bean Metabolomics: Current Knowledge and Perspectives Toward Understanding of Plant Plasticity Under Stress Condition

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