Expression profiles of hot pepper (capsicum annuum) genes under cold stress conditions

Abstract

In an attempt to determine a cold defense mechanism in plants, we have attempted to characterize changes occurring in the expression of cold-regulated transcript levels in the hot pepper (Capsicum annum), using cDNA microarray analysis, combined with Northern blot analysis. After analysing a 3.1 K hot pepper cDNA microarray, we isolated a total of 317 cold inducible genes. We selected 42 genes which were up-regulated and three genes which were down-regulated due to cold treatment, for further analysis. Among the 45 genes which appeared to be up-regulated by cold, 19 genes appeared to be simultaneously regulated by salt stress. Among the up-regulated cold-stress genes, we identified a variety of transcription factors, including: a family of 4 ethylene-responsive element binding protein (EREBP, designated CaEREBP-C1 to C4) genes, a bZIP protein (CaBZ1), RVA1, Ring domain protein, HSF1, and the WRKY (CaWRKY1) protein. As mentioned earlier, several genes appeared to be induced not only by cold stress, but also simultaneously by salt stress. These genes included: CaEREBP-C3, CaBZ1, putative trans-activator factor, NtPRp27, malate dehydrogenase, putative auxin-repressed protein, protein phosphatase (CaTPP1), SAR8.2 protein precursor, late-embryogenesis abundant protein 5 (LEA5), DNAJ protein homologue, xyloglucanendo-1,4-beta-D-gucanase precursor, PR10, and the putative non-specific lipid transfer protein StnsLTP.

The Dormancy MarkerDRM1/ARPAssociated with Dormancy but a Broader RoleIn Planta

“…An increase in DRM1/ARP expression with cold treatment or salt stress has been documented in pea [23], Capsicum annuum [17], and the close relative of Arabidopsis, B. rapa [16]. During times of eco-dormancy due to cold, it might be anticipated that DRM1/ARP expression is high, suggesting a role for DRM1/ARP in temperature signalling.…”

Evaluation of a <i>Brassica napus</i> Auxin-Repressed Gene Induced by Flea Beetle Damage and <i>Sclerotinia sclerotiorum</i> Infection

“…These include developmentrelated ARPs such as the fruit repression SAR5 from strawberry [21], the dormancy-associated SbDRM1 from sorghum and pea [22] [23], a germination-related gene from tobacco [24], a hypocotyl elongation-related RpARP from black locust [25], the auxin-induced EuNOD-ARP1 gene from Elaeagnus umbellate root nodules [26], and the cucumber CsGRP1, which accumulates on the upper L. M. Wu et al side of seedlings during gravimorphogenesis [27]. Stress-responsive ARPs include the drought/heat-induced AtARP1 from Arabidopsis [28], a gene induced by salinity and cold from hot pepper [29], five genes responsive to drought/ cold/high-salinity from chickpea [30], the salinity/drought-responsive BnARP from B. napus [31], and two genes responsive to chilling, heat shock and salt stress (BrARP1 and BrDRM1) from B. rapa [32]. However, the role of ARPs that mediate resistance or susceptibility to insects and fungi is unknown.…”

Identification of a drought- and cold-stress inducibleWRKYgene in the cold-hardyCitrusrelativePoncirus trifoliata

“…Abiotic stresses in plants induce physiological and biochemical changes controlled by regulation of gene expression. Genome-wide gene-expression studies in cold-acclimated plants have shown that expression of several hundred genes are induced in response to low temperatures in the model plant Arabidopsis thaliana (Seki et al 2001(Seki et al , 2002Fowler & Thomashow 2002;Matsui et al 2010) and economically important crops, such as rice (Rabbani et al 2003), sugarcane (Nogueira et al 2003), pepper (Hwang et al 2005), potato (Rensink et al 2005) and sunflower (Fernandez et al 2008). expression constitute a major group of genes induced in response to abiotic stresses (Seki et al 2001(Seki et al , 2002Fowler & Thomashow 2002;Sakuma et al 2002;S¸ahin-Ç evik & Moore 2006aS¸ahin-Ç evik & Moore , 2006bWei at al.…”