Expression profiles of miRNAs in the genesis of Populus euphratica Oliv. heteromorphic leaves

Zhao, Lei; Qin, Shaowei

doi:10.1007/s10725-016-0200-0

Cited by 13 publications

(19 citation statements)

References 30 publications

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“…For example, Kalve et al found that the cytoplasmic growth is mainly based on macromolecular synthesis and therefore consumes amount of energy, so that metabolism can affect cell growth [12]. P. euphratica has typical heterophylly [9,10], 140 lncRNAs involved in development process, this result indicated that these lncRNAs maybe play a key role in the P. euphratica leaf development. 109 lncRNAs involved in stress response, it was indicated that these lncRNAs involved in regulation resistance of salt, temperature and drought stresses, enable P. euphratica to live in the desert [10].…”

Section: Discussionmentioning

confidence: 99%

“…Combined Table 2 with Fig. 2, it was showed that 127 lncRNAs participate in jointly the biologic process regulation of P. euphratica by decoying miRNA 6, 8, 1,3,7,10,12,15,16,25,27,28, 30 and 32, and participate in jointly the stress response of P. euphratica; 151 lncRNAs can decoy miRNA 1, 3-10, 12-17, 19-24, 26, 27, 29 and 31-33 respectively, and play a role in P. euphratica metabolism together. Fig.…”

Section: The Functions Of Lncrnas Predicted By Mirnasmentioning

confidence: 94%

“…The tender leaves, including linear, lanceolate, oval and broad-oval leaves, were collected from P. euphratica in Alar, Xinjiang province, China. These samples were disposed immediately according to the methods of Zhao and Qin [10]. Total RNAs were extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer′s protocol.…”

Section: Plant Materials and Ncrna Sequencingmentioning

confidence: 99%

“…Then these libraries were sequenced on the Illumina sequencing platform (HiSeqTM 2500 or other platform) and 150 bp/125bp paired-end reads were generated. Small RNA libraries and Sequencing were following the procedure described by Zhao and Qin [10].…”

Section: Plant Materials and Ncrna Sequencingmentioning

confidence: 99%

“…(P. euphratica) grows almost exclusively in the desert, is the only tree species in the Taklimakan Desert (China) [9]. P. euphratica has a high tolerance for salinity, drought, cold, and wind, meanwhile also exhibits a typical heterophylly [9,10]. All above characters of P. euphratica make it one of ideal model plants for study development and stress resistance.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Functional Predictions of Long Noncoding RNAs by MiRNAs in Populus Euphratica Oliv.

Yan¹,

Zhang²,

Luo³

et al. 2018

Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)

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Long noncoding RNAs (lncRNAs) are refer as noncoding transcript RNAs and longer than 200 nucleotides (nt) in length but shorter than 10,000 nt. LncRNAs are involved in a variety of biological processes, such as development, stress response. However, the functions of lncRNAs in plants are largely unclear. MicroRNAs (miRNAs) can regulate gene expression at transcriptional or post-transcriptional level in eukaryotes by sequence complementation. At present, the research on noncoding RNAs targeted by miRNAs is seldom explored in plants. Populus euphratica Oliv. (P. euphratica) is one of ideal model plants for studying development and stress resistance. To uncover the potential functions of lncRNAs in P. euphratica, the tender leaves of P. euphratica were analyzed using sequencing, and the data including lncRNAs and miRNAs were obtained, followed by bioinformatics methods to predict the potential binding sites of 258 mature miRNAs at 4460 lncRNAs in P. euphratica. It was found that 159 lncRNAs can act as the targets of 33 miRNAs. The corrections of miRNAs-mRNAs-lncRNAs were constructed and the functions of lncRNAs were predicted according to the roles of targets (mRNAs) of corresponding miRNAs. Our results indicated that lncRNAs of P. euphratica can regulate development, metabolism, and stress response and regulation process by decoying corresponding miRNAs.

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