Expression Profiling and Functional Characterization of MicroRNAs in Apical Periodontitis

Shen, Zhen; Wichnieski, Caroline; Carneiro, Everdan; Garlet, Gustavo P.; Letra, Ariadne; Silva, Renato

doi:10.1016/j.joen.2020.11.016

Cited by 15 publications

(14 citation statements)

References 32 publications

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“…The contribution of miRNAs to bone remodeling in CAP has also been extensively studied (Figure 1). Several miRNAs have been identified in AP (Shen et al, 2021), but only a few have been thoroughly studied. Among them, mir-10A-5p has the highest expression level, and its overexpression can downregulate TNF-a mRNA levels and upregulate IL-10 (Shen et al, 2021).…”

Section: Mirnamentioning

confidence: 99%

Mechanisms of bone remodeling and therapeutic strategies in chronic apical periodontitis

Xu-tao

Wan

Liu

et al. 2022

Front. Cell. Infect. Microbiol.

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Chronic periapical periodontitis (CAP) is a typical oral disease in which periodontal inflammation caused by an odontogenic infection eventually leads to bone loss. Uncontrolled infections often lead to extensive bone loss around the root tip, which ultimately leads to tooth loss. The main clinical issue in the treatment of periapical periodontitis is the repair of jawbone defects, and infection control is the first priority. However, the oral cavity is an open environment, and the distribution of microorganisms through the mouth in jawbone defects is inevitable. The subversion of host cell metabolism by oral microorganisms initiates disease. The presence of microorganisms stimulates a series of immune responses, which in turn stimulates bone healing. Given the above background, we intended to examine the paradoxes and connections between microorganisms and jaw defect repair in anticipation of new ideas for jaw defect repair. To this end, we reviewed the microbial factors, human signaling pathways, immune cells, and cytokines involved in the development of CAP, as well as concentrated growth factor (CGF) and stem cells in bone defect repair, with the aim of understanding the impact of microbial factors on host cell metabolism to inform the etiology and clinical management of CAP.

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Section: Mirnamentioning

confidence: 99%

Mechanisms of bone remodeling and therapeutic strategies in chronic apical periodontitis

Xu-tao

Wan

Liu

et al. 2022

Front. Cell. Infect. Microbiol.

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“… 31 , 32 Thus, in this study, we produced a functional miRNA-encapsulated liposome with controlled particle size to specifically target macrophages (MT-Lips). We encapsulate miR-10a, a miRNA known to induce M2 polarization of macrophages, 33 in MT-Lip as miR-10a/MT-Lip and demonstrate an effective increase in the proportion of M2-polarized macrophages without disturbing the population of other immune cells. This phenomenon alleviates sterile-inflammation-induced uterine fibrosis in a mouse model of AS.…”

Section: Introductionmentioning

confidence: 99%

Liposome-mediated small RNA delivery to convert the macrophage polarity: A novel therapeutic approach to treat inflammatory uterine disease

Park

Han

et al. 2022

Molecular Therapy - Nucleic Acids

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“…In response to hypoxia, dental pulp cells increase the expression of hypoxia‐inducible factor 1 and then mediate the increased transcription of pro‐angiogenesis factors, including VEGF, platelet‐derived growth factor AB and angiopoietin [ 14 ]. A recent study sequencing RNA from 10 periapical periodontitis tissues also revealed that the hypoxia‐inducible factor 1 pathway was activated [ 37 ]. Therefore, it is reasonable to assume that the PLDSCs in the present study expressed higher levels of VEGF and that PLDSC‐conditioned medium induced increased tubular structure formation in HUVECs because the PLDSCs resided in a hypoxic inflammatory condition before surgery.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of periapical lesion‐derived stem cells for dental pulp tissue engineering

Mao

et al. 2021

FEBS Open Bio

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Dental pulp tissue engineering is a promising alternative treatment for pulpitis and periapical periodontitis, and dental pulp stem cells (DPSCs) are considered to be the gold standard for dental seed cell research. Periapical lesions harbor mesenchymal stem cells with the capacity for self‐renewal and multilineage differentiation. However, it remains unknown whether these periapical lesion‐derived stem cells (PLDSCs) are suitable for dental pulp tissue engineering. To investigate this possibility, PLDSCs and DPSCs were isolated using the tissue outgrowth method and cultured under identical conditions. We then performed in vitro experiments to investigate their biological characteristics. Our results indicate that PLDSCs proliferate actively in vitro and exhibit similar morphology, immunophenotype and multilineage differentiation ability as DPSCs. Simultaneously, PLDSCs exhibit stronger migrative ability and express more vascular endothelial growth factor and glial cell line‐derived neurotrophic factor than DPSCs, and PLDSC‐derived conditioned medium was more effective in tube formation assay. The mRNA expression levels of immunomodulatory genes HLA‐G , IDO and ICAM‐1 were also higher in PLDSCs. However, regarding osteo/odontogenic differentiation, PLDSCs showed weaker alkaline phosphatase staining and lower calcified nodule formation compared to DPSCs, as well as lower expression of ALP , RUNX2 and DSPP , as confirmed by a quantitative RT‐PCR. The osteo/odontogenic protein expression levels of DSPP, RUNX2, DMP1 and SP7 were also higher in DPSCs. The present study demonstrates that PLDSCs demonstrate potential use as seed cells for dental pulp regeneration, especially for achieving enhanced neurovascularization.

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Expression Profiling and Functional Characterization of MicroRNAs in Apical Periodontitis

Cited by 15 publications

References 32 publications

Mechanisms of bone remodeling and therapeutic strategies in chronic apical periodontitis

Mechanisms of bone remodeling and therapeutic strategies in chronic apical periodontitis

Liposome-mediated small RNA delivery to convert the macrophage polarity: A novel therapeutic approach to treat inflammatory uterine disease

In vitro evaluation of periapical lesion‐derived stem cells for dental pulp tissue engineering

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