Expression profiling and ontology analysis of long noncoding RNAs in post-ischemic heart and their implied roles in ischemia/reperfusion injury

Liu, Youbin; Li, Guangnan; Lu, Huimin; Li, Wei; Li, Xianglu; Liu, Huimin; Li, Xingda; Li, Tianyu; Yu, Bo

doi:10.1016/j.gene.2014.04.016

Cited by 68 publications

(62 citation statements)

References 35 publications

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“…Another mouse study reports the regulation of lncRNAs after ischemia/reperfusion injury and showed several highly upregulated (AK035396, ENSMUST00000156081, AK005401, ENSMUST00000118172) and down-regulated (uc007prv.1, AK080112, ENSMUST00000170410, AK156124) lncRNAs. 96 In human failing left ventricular samples of paired ischemic and nonischemic patients before left ventricular assist device implantation, 18 480 lncRNAs were detected; of which, 679 and 570 were differentially expressed in ischemic versus nonischemic heart failure, respectively. 97 After left ventricular assist device, ≈10% of lncRNAs were improved or normalized.…”

Section: Regulation Of Lncrnas During Myocardial Infarction and Heartmentioning

confidence: 99%

Long Noncoding RNAs in Cardiovascular Diseases

Uchida

Dimmeler

2015

Circulation Research

677

464

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Section: Regulation Of Lncrnas During Myocardial Infarction and Heartmentioning

confidence: 99%

Long Noncoding RNAs in Cardiovascular Diseases

Uchida

Dimmeler

2015

Circulation Research

677

464

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“…Rats underwent 30 min myocardial ischemia via left anterior descending artery occlusion followed by 24 h reperfusion as previously described [15]. Briefly, rats were anesthetized with sodium pentobarbital (90 mg/kg).…”

Section: Introductionmentioning

confidence: 99%

Long Non Coding RNA-UCA1 Contributes to Cardiomyocyte Apoptosis by Suppression of p27 Expression

Liu

Daliang(²,

et al. 2015

Cell Physiol Biochem

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This is an Open Access article licensed under the terms of the Creative Commons AttributionNonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only. Key WordsLong non coding RNA • UCA1 • Cardiomyocyte apoptosis • p27 Abstract Background/Aims: Urothelial carcinoma-associated 1 (UCA1) is a recently identified long non coding RNA (lncRNA). However, few studies have explored its role in cardiomyocytes after focal cardiac ischemia reperfusion injury (CIR). Methods: Rat CIR models were established using ligation of the Lower Anterior Descending artery (LAD). Cell apoptosis and reactive oxygen species (ROS) production in cardiac tissues were explored using immunohistochemistry and DHE staining. lncRNA expression patterns were detected using microarray and validated by qPCR. Cell viability and apoptosis were examined using MTT assay and flow cytometry. Results: CIR significantly induced cell apoptosis and ROS production in the rat model. The results of microarray demonstrated the reduced expression of UCA1, which was validated by qPCR. Follow-up experiments showed that UCA1 was involved in H 2 O 2 -induced cell apoptosis. We further showed that UCA1 negatively correlated with the expression of p27. Moreover, overexpression of p27 could induce primary cardiomyocyte apoptosis. Conclusions: Reduction of UCA1 levels plays a pro-apoptotic role in primary cardiomyocytes partially through stimulation of p27 protein expression. These results are in agreement with the observed levels of UCA1, p27 and apoptosis after cardiac I/R injury, suggesting that UCA1 might have an important role during I/R injury.

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“…They have been shown to exert comprehensive effects on biological processes through a variety of mechanisms (Mercer et al, 2009;Hu et al, 2012) and are thought to play important roles in the pathophysiology of several diseases, including cancer, pulmonary fibrosis and cardiovascular disease (Cao et al, 2013;Yang et al, 2013;Liu et al, 2014). Moreover, in recent studies, lncRNAs have been discovered that can act as competitive endogenous RNAs (ceRNA) of miRNAs by sharing common miRNA response elements (MREs), inhibiting miRNA activity and attenuating the repression of miRNA-target genes (Ebert and Sharp, 2010;Poliseno et al, 2010;Cesana et al, 2011).…”

Section: Introductionmentioning

confidence: 99%