Expression profiling of exosomal miRNAs derived from different stages of infection in mice infected with Echinococcus granulosus protoscoleces using high-throughput sequencing

Xiao, Jing; Zhu, Yazhou; Wu, Jianwen; Bai, Min; Xin, Yunzhuo; Wang, Qiang; Zhao, Jiaqing

doi:10.1007/s00436-022-07536-1

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…Contrarily, in CE patients, no parasite miRNAs were detected. Previously, high throughput characterizations of the profiles of circulating sRNAs were performed in experimental AE [43], experimental CE [44], and human CE [45,46]. However, parasite miRNAs were only studied in the AE experiment where authors inoculated mice with protoscoleces and identified 7 circulating miR-NAs which did not include emu-miR-87-3p.…”

Section: Discussionmentioning

confidence: 99%

“…Evidence suggests that this parasite employs both vesicular and non-vesicular pathways to export sRNAs, with a predominance of the non-vesicular route towards the external milieu, i.e., the host [34]. Regarding sRNA secretion in vivo, high throughput profiling of circulating miRNAs was performed in experimental AE [43], experimental CE [44], and human CE [45,46]. However, parasite miRNAs were only studied in the AE experiment.…”

Section: Introductionmentioning

confidence: 99%

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Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis

Cucher,

Mariconti,

Manciulli

et al. 2023

Biology

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Alveolar (AE) and cystic (CE) echinococcosis are two parasitic diseases caused by the tapeworms Echinococcus multilocularis and E. granulosus sensu lato (s. l.), respectively. Currently, AE and CE are mainly diagnosed by means of imaging techniques, serology, and clinical and epidemiological data. However, no viability markers that indicate parasite state during infection are available. Extracellular small RNAs (sRNAs) are short non-coding RNAs that can be secreted by cells through association with extracellular vesicles, proteins, or lipoproteins. Circulating sRNAs can show altered expression in pathological states; hence, they are intensively studied as biomarkers for several diseases. Here, we profiled the sRNA transcriptomes of AE and CE patients to identify novel biomarkers to aid in medical decisions when current diagnostic procedures are inconclusive. For this, endogenous and parasitic sRNAs were analyzed by sRNA sequencing in serum from disease negative, positive, and treated patients and patients harboring a non-parasitic lesion. Consequently, 20 differentially expressed sRNAs associated with AE, CE, and/or non-parasitic lesion were identified. Our results represent an in-depth characterization of the effect E. multilocularis and E. granulosus s. l. exert on the extracellular sRNA landscape in human infections and provide a set of novel candidate biomarkers for both AE and CE detection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis

Cucher,

Mariconti,

Manciulli

et al. 2023

Biology

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“…Hydatid cysts with nanobiosensors can be detected noninvasively or minimally invasively [ 25 ]. Hydatid cysts can be detected using nanoscale imaging technologies without invasive procedures in host tissues or bodily fluids [ 106 ]. Thus, not only is patient comfort enhanced, but procedural complications are minimized as well.…”

Section: Future and Prospective Of Nanobiosensorsmentioning

confidence: 99%

Advances in Detecting Cystic Echinococcosis in Intermediate Hosts and New Diagnostic Tools: A Literature Review

Hajjafari,

Sadr,

Santucciu

et al. 2024

Veterinary Sciences

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Cystic echinococcosis (CE) is a zoonotic disease affecting humans and animals. Despite a lack of clarity about many details of parasite–intermediate host interactions, the nature of the immune responses triggered by hydatid infection has revealed new perspectives. This study discusses the latest advances in elucidating the immunologic mechanism of echinococcosis and its detection and potential approaches to enhance serodiagnosis accuracy. Moreover, nanobiosensors have been evaluated according to their potential to improve treatment efficiency and aid in an early diagnosis of cystic echinococcosis. The serum of an intermediate host can diagnose CE by analyzing antibodies induced by Echinococcus granulosus. Among the most notable features of this method are its noninvasive ability and high sensitivity, both of which make it an excellent tool for clinical diagnosis. Several serological tests, including ELISAs and immunoblotting, can detect these antibodies to assess the disease’s state and determine the treatment outcome. A thorough understanding of what cross-reactivity means and the stage of the disease are crucial to interpreting serological results. Nanobiosensors have also proven better than conventional biosensors in detecting hydatid cysts. Additionally, they are highly sensitive and versatile when detecting specific biomarkers, improving diagnostic accuracy. These immunomodulatory molecules, induced by E. granulosus, are a good candidate for diagnosing cystic echinococcosis because they alter intermediate host immune responses. Hydatid cyst detection is also enhanced through nanobiosensors, which provide better accuracy.

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“…LncRNAs and their targeted mRNA molecules might play roles in the regulation of multiple signaling pathways. For example, lncRNAs are involved in biological processes such as apoptosis, terpene skeleton biosynthesis, carbon metabolism, and dorsoventral-axis formation in hydatids (Si et al, 2022;Xiao et al, 2022). Meanwhile, lncRNAs are important immune stressrelated regulators that play a key role in CE (Yu et al, 2018); e.g., lncRNA028466 mediates host immune response by regulating the expression of T-helper 1 and 2 (Th1, Th2) cytokines (Wang et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

Whole-transcriptome sequencing and bioinformatics analysis of calcified outer-layer tissue from cystic echinococcosis

S.B.

2024

Trop Biomed

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Echinococcosis is a common zoonotic disease in livestock; the type with the highest incidence is cystic echinococcosis (CE). In clinical management, patients with CE of the liver in which the cyst wall is calcified have been found to have better prognoses than those without calcification. In this study, we collected calcified and uncalcified cyst wall tissue from patients with hepatic CE and observed significant changes in the expression of 2336 messenger ribonucleic acids (mRNAs), 178 long noncoding RNAs (lncRNAs), 210 microRNAs (miRNAs), and 33 circular RNAs (circRNAs) using high-throughput sequencing (HTS). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of differentially expressed RNAs (DERNAs: DEmRNAs, DElncRNAs, DEmiRNAs, and DEcircRNAs) were performed to explore these RNAs' potential biological functions and signaling pathways. Ultimately, the results of hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining confirmed the correlation between calcification and apoptosis of the cyst wall. In summary, this study was an initial exploration of the molecular-biological mechanism underlying spontaneous calcification of the hydatid cyst wall, and it provides a theoretical basis for exploring new targets for drug treatment in CE.

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Expression profiling of exosomal miRNAs derived from different stages of infection in mice infected with Echinococcus granulosus protoscoleces using high-throughput sequencing

Cited by 4 publications

References 39 publications

Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis

Circulating Small RNA Profiling of Patients with Alveolar and Cystic Echinococcosis

Advances in Detecting Cystic Echinococcosis in Intermediate Hosts and New Diagnostic Tools: A Literature Review

Whole-transcriptome sequencing and bioinformatics analysis of calcified outer-layer tissue from cystic echinococcosis

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