Expression profiling of human fetal growth plate cartilage by EST sequencing

Tagariello, Andreas; Schlaubitz, Silke; Hankeln, Thomas; Mohrmann, Gerrit; Stelzer, Christiane; Schweizer, Anja; Hermanns, Pia; Lee, Brendan; Schmidt, Erwin R.; Winterpacht, Andreas; Zabel, Bernhard

doi:10.1016/j.matbio.2005.08.002

Cited by 11 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Levels were lower than those of cartilage collagen genes COL2A1, COL9A1, COL11A1, COL9A3, COL9A2, and COL11A2, but higher than that of COL10A1, whose expression is restricted to hypertrophic zones. A similar study using human growth plate cartilage from 20 week prenatal to 2 year postnatal samples determined that COL27A1 transcripts represented 0.05% of total and 1.1% of collagen transcripts, and again more than that of COL10A1 [7]. Because of its expression in cartilage, COL27A1 was screened for enhancer elements that regulate transcription of other collagen genes in cartilage, and two paired SOX9-responsive elements, typical of those found in other cartilage collagen genes, were identified in intron [8].…”

Section: Introductionmentioning

confidence: 88%

Type XXVII collagen at the transition of cartilage to bone during skeletogenesis

Hjorten

Hansen²,

Underwood

et al. 2007

Bone

View full text Add to dashboard Cite

COL27A1 is a member of the collagen fibrillar gene family and is expressed in cartilaginous tissues including the anlage of endochondral bone. To begin to understand its role in skeletogenesis, the temporospatial distributions of its RNA message and protein product, type XXVII collagen, were determined in developing human skeletal tissues. Laser capture microdissection and quantitative reverse-transcription polymerase chain reaction demonstrated that gene expression occurred throughout the growth plate and that it was higher in the resting and proliferative zones than in hypertrophic cartilage. Immunohistochemical analyses showed that type XXVII collagen was most evident in hypertrophic cartilage at the primary ossification center and at the growth plate and that it accumulated in the pericellular matrix. Synthesis of type XXVII collagen overlapped partly with that of type X collagen, a marker of chondrocyte hypertrophy, preceded the transition of cartilage to bone, and was associated with cartilage calcification. Immunogold electron microscopy of extracted ECM components from mouse growth plate showed that type XXVII collagen was a component of long non-banded fibrous structures, filamentous networks, and thin banded fibrils. The timing and location of synthesis suggest that type XXVII collagen plays a role during the calcification of cartilage and the transition of cartilage to bone.

show abstract

Section: Introductionmentioning

confidence: 88%

Type XXVII collagen at the transition of cartilage to bone during skeletogenesis

Hjorten

Hansen²,

Underwood

et al. 2007

Bone

View full text Add to dashboard Cite

show abstract

“…In the context of an EST-project aimed at identifying genes regulating bone growth and differentiation novel transcripts have been isolated from a human fetal growth plate cartilage cDNA library (Tagariello et al, 2005). One of these transcripts, which was selected for further analysis because of its restricted representation in EST databases, corresponds to a predicted, yet uncharacterized human transcript (C10ORF49).…”

Section: Identification and Amino Acid Sequence Of Human And Murine Ucmamentioning

confidence: 99%

Ucma — A novel secreted factor represents a highly specific marker for distal chondrocytes

et al. 2008

Self Cite

View full text Add to dashboard Cite

“…Although the complete set of genes expressed in human cartilage has not yet been described, analysis of human cartilage cDNA libraries has provided an initial in vivo picture of the cartilage transcriptome [3-6]. These investigations have also identified expression of both known and novel genes.…”

Section: Introductionmentioning

confidence: 99%

Cartilage-selective genes identified in genome-scale analysis of non-cartilage and cartilage gene expression

et al. 2007

View full text Add to dashboard Cite

Background: Cartilage plays a fundamental role in the development of the human skeleton. Early in embryogenesis, mesenchymal cells condense and differentiate into chondrocytes to shape the early skeleton. Subsequently, the cartilage anlagen differentiate to form the growth plates, which are responsible for linear bone growth, and the articular chondrocytes, which facilitate joint function. However, despite the multiplicity of roles of cartilage during human fetal life, surprisingly little is known about its transcriptome. To address this, a whole genome microarray expression profile was generated using RNA isolated from 18-22 week human distal femur fetal cartilage and compared with a database of control normal human tissues aggregated at UCLA, termed Celsius.

show abstract

Expression profiling of human fetal growth plate cartilage by EST sequencing

Cited by 11 publications

References 44 publications

Type XXVII collagen at the transition of cartilage to bone during skeletogenesis

Type XXVII collagen at the transition of cartilage to bone during skeletogenesis

Ucma — A novel secreted factor represents a highly specific marker for distal chondrocytes

Cartilage-selective genes identified in genome-scale analysis of non-cartilage and cartilage gene expression

Contact Info

Product

Resources

About