Expression profiling of ovarian BMP antagonists reveals the potential interaction between TWSG1 and the chordin subfamily in the ovary

Wang, Ying Wen; Wu, Chia Hsu; Lin, Ting Yu; Luo, Chwan Yau

doi:10.1016/j.mce.2021.111457

Cited by 3 publications

(5 citation statements)

References 38 publications

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“…We further verified the function of BAMBI by adding recombinant BMP2 protein. Members of the TGF‐β superfamily play an important role in the regulation of ovarian growth and function (Wang et al, 2021). The BMP signaling system is present in the follicle and plays a role in the development and function of the follicle (Fatehi et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that BAMBI may play a role in cholesterol transport and pregnenolone conversion to P 4 in LGCs. important role in the regulation of ovarian growth and function (Wang et al, 2021). The BMP signaling system is present in the follicle and plays a role in the development and function of the follicle (Fatehi et al, 2005).…”

Section: Effect Of Bmp2 On Steroid Hormones and Related Genesmentioning

confidence: 99%

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Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep

Zhang

Guo

et al. 2023

Molecular Reproduction Devel

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Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) regulates mammalian ovarian follicle growth and maturation; however, its effect on luteinized granulosa cells (LGCs) in sheep ovarian follicles remains unknown.Here we explored the regulatory role of LGC functions and steroid hormone synthesis by BAMBI. Multiple sequence alignment revealed that the sheep BAMBI gene sequence was relatively conserved. Sheep LGCs were strongly positive for BAMBI. LGC proliferation increased when BAMBI was silenced and decreased when BAMBI was overexpressed. After BAMBI overexpression, the expression of CASP3, CASP8, CASP9, and BAX significantly increased, whereas that of BCL2 and the ratio of BCL2/BAX expression decreased. The opposite was observed after BAMBI silencing. CDKN1A, CCND1, and CCND2 were downregulated with BAMBI overexpression and upregulated with BAMBI silencing. Expression of steroid hormonerelated genes (CYP11A1, STAR, and 3BHSD), except CYP19A1, significantly increased after BAMBI overexpression. Moreover, estrogen and progesterone secretion increased after BAMBI overexpression and decreased after BAMBI interference.The effect of the exogenous addition of bone morphogenetic protein 2 (BMP2) on GCs was similar to that of BAMBI overexpression. In conclusion, BAMBI can regulate the proliferation and steroid hormone synthesis of sheep LGCs, and BMP2 can affectLGCs as an activator of BAMBI. These findings provide a basis for further research on the physiological role of BAMBI.

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Section: Discussionmentioning

confidence: 99%

Section: Effect Of Bmp2 On Steroid Hormones and Related Genesmentioning

confidence: 99%

Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep

Zhang

Guo

et al. 2023

Molecular Reproduction Devel

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“…Juvenile mouse ovaries also exhibited consistently high TWSG1 expression, which was not affected by FSH exposure in the granulosa cells of developing follicles [ 59 ]. Wang et al found that TWSG1 and Nbl1 were the most abundant BMP antagonists expressed in rodent and human ovaries [ 60 ]. TWSG1 has been reported to have synergistic action with the Chordin subfamily, including CHRD and CHRDL1, the genes of which also showed moderate expression in the mammalian ovary.…”

Section: Twsg1 and Folliculogenesismentioning

confidence: 99%

“…BMP2, BMP4, BMP7, GDF5, and activin A have been said to have an antagonizing ability towards CHRD, which has synergic effects with TWSG1 [ 59 ]. TWSG1 is the most abundant BMP antagonist in mouse ovaries [ 60 ]. TWSG1 colocalizes with Chordin within the theca cells, whereas it colocalizes with Chordin-like 1 in the developing granulosa cells of mouse ovaries [ 60 ], suggesting the differential roles of TWSG1 in each ovarian compartment.…”

Section: Twsg1 and Folliculogenesismentioning

confidence: 99%

“…TWSG1 is the most abundant BMP antagonist in mouse ovaries [ 60 ]. TWSG1 colocalizes with Chordin within the theca cells, whereas it colocalizes with Chordin-like 1 in the developing granulosa cells of mouse ovaries [ 60 ], suggesting the differential roles of TWSG1 in each ovarian compartment. Some data suggest that TWSG1 may work coordinately with CHRD within theca/interstitial shells and also with CHRDL1 in developing granulosa cells.…”

Section: Twsg1 and Folliculogenesismentioning

confidence: 99%

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Multifaceted Functions of TWSG1: From Embryogenesis to Cancer Development

Suzuki

Fukuda

2022

IJMS

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Bone morphogenetic proteins (BMPs) play an important role in development. Twisted gastrulation BMP signaling modulator 1 (TWSG1) was initially identified as a regulator of the dorsoventral axis formation in Drosophila. The mechanism of BMP signaling modulation by TWSG1 is complex. TWSG1 inhibits BMP signaling by binding to BMP ligands including BMP4, whereas it enhances signaling by interacting with Chordin, a BMP antagonist. Therefore, TWSG1 can act as both a BMP agonist and antagonist. TWSG1 has various functions ranging from embryogenesis to cancer progression. TWSG1 knockout mice showed neural, craniofacial, and mammary defects. TWSG1 also regulated erythropoiesis and thymocyte development. Furthermore, the relationship between TWSG1 and cancer has been elucidated. Allelic loss of TWSG1 was detected in colorectal cancer. TWSG1 expression was upregulated in papillary thyroid carcinoma and glioblastoma but downregulated in gastric and endometrial cancers. TWSG1 suppressed BMP7-enhanced sphere formation and migration in endometrial cancer cells, indicating its tumor-suppressive role. Further studies are required to clarify the TWSG1 function and its association with BMP signaling in cancer development. Finally, TWSG1 is abundantly expressed in human and mouse ovaries and sustains follicular growth in rodent ovaries. Thus, TWSG1 has various functions ranging from fertility to cancer. Therefore, TWSG1 signaling modulation may be beneficial in treating specific diseases such as cancer.

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Long-Term In Vitro Culture Alters Gene Expression Pattern of Genes Involved in Ontological Groups Representing Cellular Processes

Zgórecka,

Kranc,

Blatkiewicz

et al. 2024

IJMS

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The oviduct provides an optimal environment for the final preparation, transport, and survival of gametes, the fertilization process, and early embryonic development. Most of the studies on reproduction are based on in vitro cell culture models because of the cell’s accessibility. It creates opportunities to explore the complexity of directly linked processes between cells. Previous studies showed a significant expression of genes responsible for cell differentiation, maturation, and development during long-term porcine oviduct epithelial cells (POECs) in vitro culture. This study aimed at establishing the transcriptomic profile and comprehensive characteristics of porcine oviduct epithelial cell in vitro cultures, to compare changes in gene expression over time and deliver information about the expression pattern of genes highlighted in specific GO groups. The oviduct cells were collected after 7, 15, and 30 days of in vitro cultivation. The transcriptomic profile of gene expression was compared to the control group (cells collected after the first day). The expression of COL1A2 and LOX was enhanced, while FGFBP1, SERPINB2, and OVGP1 were downregulated at all selected intervals of cell culture in comparison to the 24-h control (p-value < 0.05). Adding new detailed information to the reproductive biology field about the diversified transcriptome profile in POECs may create new future possibilities in infertility treatments, including assisted reproductive technique (ART) programmes, and may be a valuable tool to investigate the potential role of oviduct cells in post-ovulation events.

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Expression profiling of ovarian BMP antagonists reveals the potential interaction between TWSG1 and the chordin subfamily in the ovary

Cited by 3 publications

References 38 publications

Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep

Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep

Multifaceted Functions of TWSG1: From Embryogenesis to Cancer Development

Long-Term In Vitro Culture Alters Gene Expression Pattern of Genes Involved in Ontological Groups Representing Cellular Processes

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