Expression Profiling of Translation-associated Genes in Sporulating<i>Bacillus subtilis</i>and Consequence of Sporulation by Gene Inactivation

Ohashi, Yoshiaki; Inaoka, Tsukasa; Kasai, Koji; Ito, Yasuhiro; Okamoto, Susumu; Satsu, Hideo; Tozawa, Yuzuru; Kawamura, Fujio; Ochi, Kozo

doi:10.1271/bbb.67.2245

Cited by 36 publications

(35 citation statements)

References 37 publications

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“…The continued expression of these genes most likely reflects the ongoing need for metabolic capacity required during the formation of the mature spore. Expression of translationassociated genes during sporulation has already been described by Ohashi et al (2003). In accordance with those authors, we also observed increased expression of some ribosome-associated genes, such as ctc.…”

Section: Differentiation Of the Compartment-specific Sporulation Regusupporting

confidence: 92%

“…In accordance with those authors, we also observed increased expression of some ribosome-associated genes, such as ctc. However, comparing the observations of the present study with other investigations, one has to recall that the expression analysis of this study was confined to the period following resuspension in sporulation medium, and thus initial decreases in overall expression of ribosomal proteins immediately upon nutritional shift-down (Ohashi et al, 2003) might have been missed here.…”

Section: Differentiation Of the Compartment-specific Sporulation Regumentioning

confidence: 50%

“…Recent studies have shown that core RNA polymerase and s A levels remain constant during the transition from vegetative growth to sporulation (Fujita, 2000), and several lines of evidence suggest that s A -directed transcription takes place and is important even at late stages of sporulation (Fujita, 2000;Fukushima et al, 2003;Li & Piggot, 2001;Ohashi et al, 2003). A metabolic-pathwayoriented inspection of the temporal expression pattern revealed that certain classes of housekeeping genes continued to be expressed at a high level during sporulation, at least until 150 min after the initiation of sporulation.…”

Section: Differentiation Of the Compartment-specific Sporulation Regumentioning

confidence: 99%

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Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

et al. 2005

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Temporal and compartment-specific control of gene expression during sporulation in Bacillus subtilis is governed by a cascade of four RNA polymerase subunits. s F in the prespore and s E in the mother cell control early stages of development, and are replaced at later stages by s G and s K , respectively. Ultimately, a comprehensive description of the molecular mechanisms underlying spore morphogenesis requires the knowledge of all the intervening genes and their assignment to specific regulons. Here, in an extension of earlier work, DNA macroarrays have been used, and members of the four compartment-specific sporulation regulons have been identified. Genes were identified and grouped based on: i) their temporal expression profile and ii) the use of mutants for each of the four sigma factors and a bofA allele, which allows s K activation in the absence of s G . As a further test, artificial production of active alleles of the sigma factors in non-sporulating cells was employed. A total of 439 genes were found, including previously characterized genes whose transcription is induced during sporulation: 55 in the s F regulon, 154 s E -governed genes, 113 s G -dependent genes, and 132 genes under s K control. The results strengthen the view that the activities of s F , s E , s G and s K are largely compartmentalized, both temporally as well as spatially, and that the major vegetative sigma factor (s A ) is active throughout sporulation. The results provide a dynamic picture of the changes in the overall pattern of gene expression in the two compartments of the sporulating cell, and offer insight into the roles of the prespore and the mother cell at different times of spore morphogenesis.

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Section: Differentiation Of the Compartment-specific Sporulation Regusupporting

confidence: 92%

Section: Differentiation Of the Compartment-specific Sporulation Regumentioning

confidence: 50%

Section: Differentiation Of the Compartment-specific Sporulation Regumentioning

confidence: 99%

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Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

et al. 2005

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“…A previous study had shown that inactivation of ctc, which encodes a homologue of L25, causes a temperature-sensitive sporulation phenotype in B. subtilis (55). In addition, the sporulation frequency of an rpmGB::pMutinT3-rpmGB mutant, in which transcription of the rpmGB (L33.2) gene is under the control of an isopropyl-␤-D-thiogalactoside (IPTG)-regulated promoter, decreased slightly at 47°C (41). However, the involvement of these ribosomal proteins in sporulation remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Ribosomal Protein Genes in Bacillus subtilis Reveals Importance of Each Ribosomal Protein for Cell Proliferation and Cell Differentiation

et al. 2012

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bAmong the 57 genes that encode ribosomal proteins in the genome of Bacillus subtilis, a Gram-positive bacterium, 50 genes were targeted by systematic inactivation. Individual deletion mutants of 16 ribosomal proteins (L1, L9, L15, L22, L23, L28, L29, L32, L33.1, L33.2, L34, L35, L36, S6, S20, and S21) were obtained successfully. In conjunction with previous reports, 22 ribosomal proteins have been shown to be nonessential in B. subtilis, at least for cell proliferation. Although several mutants that harbored a deletion of a ribosomal protein gene did not show any significant differences in any of the phenotypes that were tested, various mutants showed a reduced growth rate and reduced levels of 70S ribosomes compared with the wild type. In addition, severe defects in the sporulation frequency of the ⌬rplA (L1) mutant and the motility of the ⌬rpsU (S21) mutant were observed. These data provide the first evidence in B. subtilis that L1 and S21 are required for the progression of cellular differentiation.

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“…Incorporation of L27 or L36 is not likely to be the essential function(s) of RbgA, YsxC, or YphC, as L27 and L36 are dispensable for growth in both E. coli and B. subtilis (22,23,27). Conversely, mutants defective in L16 have not been isolated, suggesting that L16 is essential.…”

Section: Discussionmentioning

confidence: 99%

Multiple GTPases Participate in the Assembly of the Large Ribosomal Subunit in Bacillus subtilis

et al. 2006

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Expression Profiling of Translation-associated Genes in SporulatingBacillus subtilisand Consequence of Sporulation by Gene Inactivation

Cited by 36 publications

References 37 publications

Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

Inactivation of Ribosomal Protein Genes in Bacillus subtilis Reveals Importance of Each Ribosomal Protein for Cell Proliferation and Cell Differentiation

Multiple GTPases Participate in the Assembly of the Large Ribosomal Subunit in Bacillus subtilis

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