Expression, purification and identification of isotope-labeled recombinant cystatin C protein in Escheichia coli intended for absolute quantification using isotope dilution mass spectrometry

Zhang, Qiaoxuan; Cai, Zhiliang; Lin, Haibiao; Han, Liqiao; Yan, Jun; Wang, Jianbing; Ke, Peifeng; Zhang, Junhua; Huang, Xianzhang

doi:10.1016/j.pep.2020.105785

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…To overcome the current limitations of the cysC measurement system, methodological improvements are required. First, the most promising candidate, isotope dilution mass spectrometry (IDMS), will be a reference measurement method for cysC [38,39], and current commercial methods should be calibrated to be traceable with respect to the IDMS method. Second, it is essential to develop novel reference materials for cysC that are assigned clinically useful values and do not require dilution.…”

Section: Discussionmentioning

confidence: 99%

Effect of Two Cystatin C Reagents and Four Equations on Glomerular Filtration Rate Estimations After Standardization

et al. 2023

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Background: Serum cystatin C (cysC), which is less affected by sex, race, and muscle mass than creatinine, is a useful biomarker of the estimated glomerular filtration rate (eGFR). The standardization of cysC measurements remains controversial, although a certified reference material (ERM-DA471/IFCC) is available. Moreover, the effect of combinations of cysC reagents and equations for eGFR is unclear.Methods: We conducted a simulation analysis of cysC measured using two reagents standardized against ERM-DA471/IFCC-Gentian cystatin C immunoassay (Gentiancys; Gentian AS, Moss, Norway) and Roche Tina-quant Cystatin C Gen.2 (Rochecys; Roche, Mannheim, Germany)-on a Cobas c702 system (Roche) and eGFR generated by eight combinations of four equations: 2012 cystatin C-based Chronic Kidney Disease Epidemiology Collaboration equation (CKD-EPIcys); the Caucasian, Asian, pediatric, and adult equation (CAPAeq); full age spectrum equation (FASeq); and 2023 cystatin C-based European Kidney Function Consortium equation (EKFCcys).Results: A total of 148 participants (mean age, 60.5 ± 14.5 years; 43% female) were enrolled. The mean cysC was 1.72 ± 1.44 mg/L for Gentiancys and 1.71 ± 1.35 mg/L for Rochecys. Regression analysis showed concordance between the reagents within 0.85-4.40 mg/L when using ± 7.61% total allowable error. Lin's concordance correlation coefficient of eGFR, by combining the measuring system and equation, varied from 0.73 to 1.00. Conclusions:The equivalence of cysC values at low concentrations ( < 0.85 mg/L) between the two reagents was unsatisfactory. Results obtained with different measurement systems could lead to larger differences in eGFR varying with the combination.

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Section: Discussionmentioning

confidence: 99%

Effect of Two Cystatin C Reagents and Four Equations on Glomerular Filtration Rate Estimations After Standardization

et al. 2023

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“…Another method for identifying isotope-labeled proteins is isotope dilution mass spectrometry. An isotope-labeled 15N-Cys C protein in E. coli was effectively discovered using this method ( 93 ). TMT ( 94 , 95 ) is a method for quantifying proteins/peptides using tissue, serum, plasma, or other body fluid samples from the affected/diseased area.…”

Section: Role Of Proteomics To Unravel Bacterial Pathogenesismentioning

confidence: 99%

Proteomics approaches: A review regarding an importance of proteome analyses in understanding the pathogens and diseases

Zubair

Wang

et al. 2022

Front. Vet. Sci.

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Proteomics is playing an increasingly important role in identifying pathogens, emerging and re-emerging infectious agents, understanding pathogenesis, and diagnosis of diseases. Recently, more advanced and sophisticated proteomics technologies have transformed disease diagnostics and vaccines development. The detection of pathogens is made possible by more accurate and time-constrained technologies, resulting in an early diagnosis. More detailed and comprehensive information regarding the proteome of any noxious agent is made possible by combining mass spectrometry with various gel-based or short-gun proteomics approaches recently. MALDI-ToF has been proved quite useful in identifying and distinguishing bacterial pathogens. Other quantitative approaches are doing their best to investigate bacterial virulent factors, diagnostic markers and vaccine candidates. Proteomics is also helping in the identification of secreted proteins and their virulence-related functions. This review aims to highlight the role of cutting-edge proteomics approaches in better understanding the functional genomics of pathogens. This also underlines the limitations of proteomics in bacterial secretome research.

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A protein standard absolute quantification strategy for enhanced absolute quantification of ricin in complex matrices using in vitro synthesized mutant holoprotein as internal standard by ultra-high-performance liquid chromatography-tandem mass spectrometry

Liang,

Ma,

Yang

et al. 2023

Journal of Chromatography A

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Expression, purification and identification of isotope-labeled recombinant cystatin C protein in Escheichia coli intended for absolute quantification using isotope dilution mass spectrometry

Cited by 5 publications

References 35 publications

Effect of Two Cystatin C Reagents and Four Equations on Glomerular Filtration Rate Estimations After Standardization

Effect of Two Cystatin C Reagents and Four Equations on Glomerular Filtration Rate Estimations After Standardization

Proteomics approaches: A review regarding an importance of proteome analyses in understanding the pathogens and diseases

A protein standard absolute quantification strategy for enhanced absolute quantification of ricin in complex matrices using in vitro synthesized mutant holoprotein as internal standard by ultra-high-performance liquid chromatography-tandem mass spectrometry

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