Expression, Purification of Recombinant Human Mitochondrial Transcription Termination Factor 3 (hMTERF3) and Preparation of Polyclonal Antibody Against hMTERF3

Xiong, Wei; Luo, Yong-Hui; Zhang, Cuixiang; Tan, Dezhi; Zuo, Shi

doi:10.1007/s12010-012-9754-0

Cited by 4 publications

(5 citation statements)

References 23 publications

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“…Overexpression of MTERF2 can prohibit cell proliferation [95], but the mechanism has not been well defined so far. MTERF3 is the most conserved member of the MTERF family with the negative regulation on mammalian mitochondrial DNA transcription [96]. DEAD-box helicases are fundamental in DNA and RNA metabolism such as replication, repair, recombination, transcription, translation, ribosome biogenesis, and splicing, which regulate and control plant growth and development [97, 98].…”

Section: Discussionmentioning

confidence: 99%

A high-density genetic map constructed using specific length amplified fragment (SLAF) sequencing and QTL mapping of seed-related traits in sesame (Sesamum indicum L.)

Zhang

Wei

et al. 2019

BMC Plant Biol

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BackgroundSesame (Sesamum indicum L., 2n = 2x = 26) is an important oilseed crop with high oil content but small seed size. To reveal the genetic loci of the quantitative seed-related traits, we constructed a high-density single nucleotide polymorphism (SNP) linkage map of an F2 population by using specific length amplified fragment (SLAF) technique and determined the quantitative trait loci (QTLs) of seed-related traits for sesame based on the phenotypes of F3 progeny.ResultsThe genetic map comprised 2159 SNP markers distributed on 13 linkage groups (LGs) and was 2128.51 cM in length, with an average distance of 0.99 cM between adjacent markers. QTL mapping revealed 19 major-effect QTLs with the phenotypic effect (R2) more than 10%, i.e., eight QTLs for seed coat color, nine QTLs for seed size, and two QTLs for 1000-seed weight (TSW), using composite interval mapping method. Particularly, LG04 and LG11 contained collocated QTL regions for the seed coat color and seed size traits, respectively, based on their close or identical locations. In total, 155 candidate genes for seed coat color, 22 for seed size traits, and 54 for TSW were screened and analyzed.ConclusionsThis report presents the first QTL mapping of seed-related traits in sesame using an F2 population. The results reveal the location of specific markers associated with seed-related traits in sesame and provide the basis for further seed quality traits research.

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Section: Discussionmentioning

confidence: 99%

A high-density genetic map constructed using specific length amplified fragment (SLAF) sequencing and QTL mapping of seed-related traits in sesame (Sesamum indicum L.)

Zhang

Wei

et al. 2019

BMC Plant Biol

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“…Primer Premier 5.0 software was used to design PCR primers according to sequences provided by GenBank, and the primer homology was compared using the NCBI/Primer-BLAST website ( 20 ). A 100 mg frozen breast cancer and noncancerous breast tissues samples was used for the isolation of total RNA, with TRIzol solution.…”

Section: Methodsmentioning

confidence: 99%

Expression of MTERF3 gene in breast carcinoma and the relationship with clinicopathological characteristics

Wang¹,

Zi²,

Sun³

et al. 2020

Transl Cancer Res TCR

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Background Mitochondrial transcription termination factor 3 (MTERF3) is a negative regulator of mitochondrial transcription. It is a modular factor involves in mitochondrial ribosome biogenesis and protein synthesis. However, the association between MTERF3 and breast cancers remains largely unknown. The aim of this study was to investigate the expression of MTERF3 in breast carcinoma and to analyze its clinicopathological significance, and to examine the potential prognostic value of MTERF3 in breast cancer. Methods The protein expression levels of MTERF3 in MCF7 (Luminal A), BT-474 (Luminal B), SKBR3 (HER2 overexpression), MDA-MB-468 (Basal like) and MCF10A cell lines were detected by Western blotting. Immunohistochemistry (IHC), Western blotting, and semiquantitative RT-PCR were performed to analyze the protein and mRNA expression levels of MTERF3 in 58 breast cancer tissues and 58 noncancerous breast tissues. The MTERF3 expression data and clinical information from breast cancer patients were downloaded from the TCGA dataset by using the R3.6.1 software. Then the relationship between the expression level of MTERF3 and clinicopathological characteristics and the prognostic value was analyzed. A Cox regression model was performed for the multivariate analysis of the factors that affected the prognosis of breast cancer. The association between the expression levels of MTERF3 and other mitochondrial regulatory genes was analyzed with GEPIA. Results MTERF3 is upregulated in breast cancer cell lines compared to noncancerous breast cell line. The IHC results showed that the MTERF3 protein was located in the cytoplasm, and the rate of positive expression in breast cancer tissue was significantly upregulated compared with the adjacent normal tissue. The mRNA and protein expression levels of MTERF3 in breast cancer tissues were significantly higher than that in breast tissues. Moreover, the expression of MTERF3 was significantly correlated with ER status, PR status, breast cancer molecular typing, cancer type, histological diagnosis and primary site (P<0.05). Further analysis showed MTERF3 expression was not related to prognosis. Multivariate Cox regression analysis showed that age, metastasis status and tumor type were independent prognostic factors for breast cancer patients. The expression levels of MTERF3 were positively correlated with the TFAM , TFB1M , TFB2M , MTERF1 , TEFM and MFN1 genes but negatively correlated with the MTERF4 and PINK1 genes. In addition, the expression levels of MTERF3 were not correlated with the MTERF2 gene. Conclusions MTERF3 was significantly upregulated in breast cancer cells and tissues compared with noncancerous cells and tissues. Moreover, th...

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“…MTERF3 gene amplification and upregulation are negatively correlated with overall survival in cancer patients [ 119 ]. Overexpression of the MTERF3 gene promotes tumor cell growth both in vivo and in vitro, increasing the percentage of cells in the S phase [ 120 ]. MTERF3 plays an oncogenic role in colorectal cancer development by upregulating interleukin 6 and interleukin 11 to promote colorectal cancer cell growth and enhance radiotherapy resistance [ 121 ].…”

Section: Dysregulation Of Mitochondrial Transcription Affects Tumor P...mentioning

confidence: 99%

Mitochondria transcription and cancer

Lei,

Rui,

Xiaoshuang

et al. 2024

Cell Death Discov.

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Mitochondria are major organelles involved in several processes related to energy supply, metabolism, and cell proliferation. The mitochondria function is transcriptionally regulated by mitochondria DNA (mtDNA), which encodes the key proteins in the electron transport chain that is indispensable for oxidative phosphorylation (OXPHOS). Mitochondrial transcriptional abnormalities are closely related to a variety of human diseases, such as cardiovascular diseases, and diabetes. The mitochondria transcription is regulated by the mtDNA, mitochondrial RNA polymerase (POLRMT), two transcription factors (TFAM and TF2BM), one transcription elongation (TEFM), and one known transcription termination factor (mTERFs). Dysregulation of these factors directly leads to altered expression of mtDNA in tumor cells, resulting in cellular metabolic reprogramming and mitochondrial dysfunction. This dysregulation plays a role in modulating tumor progression. Therefore, understanding the role of mitochondrial transcription in cancer can have implications for cancer diagnosis, prognosis, and treatment. Targeting mitochondrial transcription or related pathways may provide potential therapeutic strategies for cancer treatment. Additionally, assessing mitochondrial transcriptional profiles or biomarkers in cancer cells or patient samples may offer diagnostic or prognostic information.

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Expression, Purification of Recombinant Human Mitochondrial Transcription Termination Factor 3 (hMTERF3) and Preparation of Polyclonal Antibody Against hMTERF3

Cited by 4 publications

References 23 publications

A high-density genetic map constructed using specific length amplified fragment (SLAF) sequencing and QTL mapping of seed-related traits in sesame (Sesamum indicum L.)

A high-density genetic map constructed using specific length amplified fragment (SLAF) sequencing and QTL mapping of seed-related traits in sesame (Sesamum indicum L.)

Expression of MTERF3 gene in breast carcinoma and the relationship with clinicopathological characteristics

Mitochondria transcription and cancer

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