Expression sites of the collectin SP-D suggest its importance in first line host defence: Power of combining in situ hybridisation, RT-PCR and immunohistochemistry

Herías, M. Verónica; Hogenkamp, Astrid; Asten, Alphons J.A.M. van; Tersteeg, Monique H.G.; Eijk, Martin van; Haagsman, Henk P.

doi:10.1016/j.molimm.2007.02.025

Cited by 27 publications

(20 citation statements)

References 34 publications

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“…For example, SP-D was robustly expressed (17.4-fold higher) in expanded small airway epithelial cells (Fig. 3A), which is consistent with previous studies of SP-D distribution in pig, rat, and human small airways (21,29,40). Using real-time PCR and immunocytochemistry, we validated that SP-D expression was higher in excised native small airway tissue, with no SP-D-positive staining detected in large airway surface epithelia (Fig.…”

Section: Isolation Expansion and Culture Of Distal Small Airway Cellssupporting

confidence: 91%

Electrolyte transport properties in distal small airways from cystic fibrosis pigs with implications for host defense

Tang

Buonfiglio

et al. 2016

American Journal of Physiology-Lung Cellular and Molecular Phys

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While pathological and clinical data suggest that small airways are involved in early cystic fibrosis (CF) lung disease development, little is known about how the lack of cystic fibrosis transmembrane conductance regulator (CFTR) function contributes to disease pathogenesis in these small airways. Large and small airway epithelia are exposed to different airflow velocities, temperatures, humidity, and CO2 concentrations. The cellular composition of these two regions is different, and small airways lack submucosal glands. To better understand the ion transport properties and impacts of lack of CFTR function on host defense function in small airways, we adapted a novel protocol to isolate small airway epithelial cells from CF and non-CF pigs and established an organotypic culture model. Compared with non-CF large airways, non-CF small airway epithelia cultures had higher Cl− and bicarbonate (HCO3−) short-circuit currents and higher airway surface liquid (ASL) pH under 5% CO2 conditions. CF small airway epithelia were characterized by minimal Cl− and HCO3− transport and decreased ASL pH, and had impaired bacterial killing compared with non-CF small airways. In addition, CF small airway epithelia had a higher ASL viscosity than non-CF small airways. Thus, the activity of CFTR is higher in the small airways, where it plays a role in alkalinization of ASL, enhancement of antimicrobial activity, and lowering of mucus viscosity. These data provide insight to explain why the small airways are a susceptible site for the bacterial colonization.

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Section: Isolation Expansion and Culture Of Distal Small Airway Cellssupporting

confidence: 91%

Electrolyte transport properties in distal small airways from cystic fibrosis pigs with implications for host defense

Tang

Buonfiglio

et al. 2016

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Expression of SP-A and SP-D has also been reported in extra pulmonary tissues such as brain, salivary glands, lachrymal glands, heart, trachea, kidney, pancreas, thymus, spleen, gall bladder, esophagus, small intestine, large intestine, testis, prostate and urinary tract (Madsen et al, 2003;Herías et al, 2007;Breuiller-Fouché et al, 2010;Nayak et al, 2012;Schicht et al, 2015). In addition, reproductive tissues have also been shown to express both SP-A and SP-D (Sati et al, 2009;Condon et al, 2004;Yadav et al, 2011).…”

Section: Introductionmentioning

confidence: 97%

Expression of surfactant proteins SP-A and SP-D in murine decidua and immunomodulatory effects on decidual macrophages

et al. 2016

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“…Therefore, it is assumed that SP-A and SP-D are also involved in innate host defense of the upper airways. Although SP-A and SP-D are referred to as 'lung collectins', especially SP-D is also expressed in many other tissues including gastric and intestinal mucosae [7,8] . The expression of SP-D at many extrapulmonary mucosal sites suggests that this collectin plays a more general role in local innate host defense.…”

Section: Sp-a and Sp-dmentioning

confidence: 99%

Surfactant Collectins and Innate Immunity

et al. 2008

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Respiratory pathogens encounter various lines of defenses before infection of the host is established. The innate immune response represents an important first-line protection mechanism against potentially pathogenic microorganisms during early stages of infection of the naive host. Important players in this host defense system are ‘collectins’, a class of soluble innate immune proteins. Well-characterized members of the collectin family are the surfactant proteins A (SP-A) and D (SP-D). These collectins are expressed in the lung and also in extrapulmonary mucosal tissues. Collectins are secreted as multimers resulting in trimeric clustering of the lectin domains which enables recognition of evolutionary conserved sugar patterns present on the surface of a large variety of pathogens. Binding to collectins may lead to direct agglutination and neutralization of pathogens, to opsonization in order to present bound microbes directly to phagocytes, to modulation of the inflammatory response and to regulation of dendritic cell and T cell functions. In pulmonary tissue, this early acute-phase-like response can be regarded as a crucial layer of protection against a vast array of pathogens that escape the physical barriers and threaten to infect the delicate respiratory epithelium. An important clinical application may be the inhalation, or instillation of collectin-based drugs as part of surfactant therapy, to prevent and treat infectious and inflammatory diseases of newborn infants.

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Expression sites of the collectin SP-D suggest its importance in first line host defence: Power of combining in situ hybridisation, RT-PCR and immunohistochemistry

Cited by 27 publications

References 34 publications

Electrolyte transport properties in distal small airways from cystic fibrosis pigs with implications for host defense

Electrolyte transport properties in distal small airways from cystic fibrosis pigs with implications for host defense

Expression of surfactant proteins SP-A and SP-D in murine decidua and immunomodulatory effects on decidual macrophages

Surfactant Collectins and Innate Immunity

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