Expression und Untersuchung von Pflanzenproteinen mit Leishmania tarentolae

Abstract

Glycosylation is an important post-translational modification that can be found in many eukaryotic proteins. Since it can be crucial for protein structure and function, properly glycosylated proteins are of paramount importance for crystal structure determination. The eukaryotic expression system LEXSY, based on the protozoan Leishmania tarentolae, has been used to express and crystallize fully glycosylated Arabidopsis thaliana legumain γ and elucidate the role of glycosylation for its function.