Expressional Alterations of Fibrillin-1 during Wound Healing of Human Dental Pulp

Yoshiba, Nagako; Yoshiba, Kunihiko; Ohkura, Naoto; Hosoya, Akihiro; Shigetani, Yoshimi; Yamanaka, Yusuke; Izumi, Noriaki; Nakamura, Hiroaki; Okiji, Takashi

doi:10.1016/j.joen.2011.09.016

Cited by 16 publications

(14 citation statements)

References 37 publications

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“…According to previous reports [31][32][33][34], 4 targets related to odontogenesis were identified from the differentially expressed mRNAs. Among the 4 mRNAs, DNA damage inducible transcript 3 (DDIT3) and FBN1 were upregulated, while rhodopsin (RHO) and NKD inhibitor of WNT signaling pathway 2 (NKD2) were downregulated.…”

Section: Regulatory Network Of Mirnas/odontogenesis-related Genesmentioning

confidence: 99%

Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells

et al. 2020

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Background: Long noncoding RNAs (lncRNAs) play an important role in the multiple differentiations of mesenchymal stem cells (MSCs). However, few studies have focused on the regulatory mechanism of lncRNAs in the odontogenic differentiation of human dental pulp stem cells (hDPSCs). Methods: hDPSCs were induced to differentiate into odontoblasts in vitro, and the expression profiles of lncRNAs, microRNAs (miRNAs), and messenger RNAs (mRNAs) in differentiated and undifferentiated cells were obtained by microarray. Bioinformatics analyses including Gene Ontology (GO) analysis, pathway analysis, and binding site prediction were performed for functional annotation of lncRNA. miRNA/odontogenesis-related gene networks and lncRNA-associated ceRNA networks were constructed. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to verify the expression of selected genes. RNA fluorescence in situ hybridization (FISH), qRT-PCR, and western blot analysis were used to explore the location and function of lncRNA-G043225. Dual-luciferase reporter assay was performed to confirm the binding sites of miR-588 with G043225 and Fibrillin 1 (FBN1). Results:We identified 132 lncRNAs, 114 miRNAs, and 172 mRNAs were differentially expressed. GO analysis demonstrated that regulation of the neurogenic locus notch homolog (Notch), Wnt, and epidermal growth factor receptor (ERBB) signaling pathways and activation of mitogen-activated protein kinase (MAPK) activity were related to odontogenic differentiation. Pathway analysis indicated that the most significant pathway was the forkhead box O (FoxO) signaling pathway, which is related to odontogenic differentiation. Two odontogenesis-related genecentered lncRNA-associated ceRNA networks were successfully constructed. The qRT-PCR validation results were consistent with the microarray analysis. G043225 mainly locating in cytoplasm was proved to promote the odontogenic differentiation of hDPSCs via miR-588 and FBN1. (Continued on next page)Conclusion: This is the first study revealing lncRNA-associated ceRNA network during odontogenic differentiation of hDPSCs using microarray, and it could provide clues to explore the mechanism of action at the RNA-RNA level as well as novel treatments for dentin regeneration based on stem cells.

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Section: Regulatory Network Of Mirnas/odontogenesis-related Genesmentioning

confidence: 99%

Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells

et al. 2020

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“…Severe inflammation was observed in some teeth treated with enamel matrix derivative. Records excluded (n = 9) due to: •no examination of hard tissue (Piva et al 2006, D´Arcangelo et al 2007, Fernandes et al 2008, Pereira et al 2009, Yoshiba et al 2012, Silva et al 2013 •full pulpotomy (Chacko & Kurikose 2006) •no pulp exposure (Mousavinasab et al 2008) •data impossible to assess (Silva et al 2006a) Full-text articles assessed for eligibility (n = 31)…”

Section: Enamel Matrix Derivative-based Materialsmentioning

confidence: 99%

Formation of a hard tissue barrier after experimental pulp capping or partial pulpotomy in humans: an updated systematic review

2015

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The aim was to update a systematic review of pulp capping and partial pulpotomy by Olsson et al. (2006), by evaluating new evidence on formation of a hard tissue barrier after pulp capping and partial pulpotomy of experimental exposures in humans. PubMed (01-01-2005 to 01-03-2014) and CENTRAL were searched using specific keywords. Hand searches were made and the level of evidence for each included article was evaluated by the authors. The evidence of the conclusions was graded as strong, moderately strong, limited or insufficient. The initial search in PubMed yielded 215 abstracts. Hand searches of reference lists yielded no additional original scientific articles. After a selection process and interpretation, 22 articles were included and rated for level of evidence: no article was rated as high and seven as moderate. Overall the methodological quality of studies has improved since the previous systematic review was published in 2006. The conclusions are that there is limited scientific evidence that application of calcium hydroxide or mineral trioxide aggregate to an exposed pulp frequently results in formation of a hard tissue barrier, whereas adhesives or enamel matrix derivatives do not. There is insufficient scientific evidence that mineral trioxide aggregate promotes hard tissue formation more frequently than calcium hydroxide.

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“…However, the extracted tooth contained not only dentin but also enamel and dental pulp tissues; therefore, the stimulation of bone formation was not directly related to the effect of matrix proteins in the dentin. The growth factors contained in the pulp tissue would stimulate healing of the socket 18,19) . In this study, the presence of the particles would sustain the size effect and biocompatibility of dentin.…”

Section: Ryohei Matsukawa Et Al: Immunohistological Observation Of Mmentioning

confidence: 99%

Immunohistological Observation of Milled Teeth in a Rat Mandibular Incisor Extraction Socket

Matsukawa

Ozawa

Kondo

et al. 2014

J. Hard Tissue Biology.

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Recycling unnecessary teeth for use as a bone grafting material should be a valuable approach to enhance the bone-forming process in alveolar bone defects and to reduce material costs. However, the process of bone formation using grafting material is not fully understood. The aim of this study was to assess the fate of material including milled teeth by immunohistological observation in a rat incisor extraction model. Freezemilled incisor material was injected into an extraction socket in a rat mandible bone. Four weeks after the operation, the mandible was sampled and frozen sections were prepared by the sticking film method, after which DMP-1 and CD68 were visualized by immunohistochemical staining. The results revealed that milled tooth particles remained around the socket at four weeks post-operation, and showed different reactions depending on the particle size. Small particles (3-10 m) were phagocytosed by CD68-positive cells, whereas bone regeneration occurred around large (30-60 m) particles. These results suggest that the injection of crushed teeth could provide an optimum scaffold and growth factors for bone formation in extraction sockets.

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Expressional Alterations of Fibrillin-1 during Wound Healing of Human Dental Pulp

Cited by 16 publications

References 37 publications

Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells

Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells

Formation of a hard tissue barrier after experimental pulp capping or partial pulpotomy in humans: an updated systematic review

Immunohistological Observation of Milled Teeth in a Rat Mandibular Incisor Extraction Socket

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