Expressional and epigenetic alterations of placental matrix metalloproteinase 9 in preeclampsia

Wang, Zhuoqun; Liu, Shuang; Liu, Chunmei; Zhao, Bin; Pei, Kaiyan; Liu, Tian; Ma, Xu

doi:10.1080/09513590903184100

Cited by 10 publications

(11 citation statements)

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“…Our data indicates lower CpG methylation in the promoter region of MMP‐9 gene in the preterm placenta as compared to term. A previous study in preeclampsia, has shown hypomethylation of a CpG near the transcription start site in MMP‐9 gene correlating with its over‐expression . Although we observed hypomethylation in the MMP‐9 promoter region in the preterm placenta, the mRNA levels were comparable between the term and preterm group, which suggests that the MMP‐9 gene expression may not be controlled by the CpGs in the promoter region studied.…”

Section: Discussioncontrasting

confidence: 60%

Investigating the expression of MMPs and TIMPs in preterm placenta and role of CpG methylation in regulating MMP‐9 expression

Sundrani

Narang

Mehendale³

et al. 2017

IUBMB Life

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Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) are crucial to the processes of normal labor and parturition. We have previously reported aberrant protein levels of MMPs in placenta of women delivering preterm as compared to term. In this study, we examine the mRNA levels of MMPs (MMP-1, MMP-2, and MMP-9) and TIMPs (TIMP-1, TIMP-2) in the placenta from women delivering preterm as compared with term and further study the promoter DNA methylation of the MMP-9 gene in a sub-sample of term and preterm placentae. A total of 110 women were included in the study; 56 delivered term and 54 delivered preterm. MMP and TIMP mRNA levels were determined by Taqman-based qPCR. Promoter CpG methylation of MMP-9 gene was studied on a subset of 10 term and 8 preterm placenta using Epitect Methyl-II PCR assay kit. The mRNA levels of MMP-1,-2 were higher and those of TIMP-1,-2 were lower in the placentae of women delivering preterm. MMP-9 levels were comparable between the two groups. Among women undergoing spontaneous vaginal deliveries, higher mRNA levels of MMP-1, -2 and -9 were seen in the placentae of those delivering preterm as compared to term. Similar results were seen in women undergoing preterm labor as compared to term. MMP-9 gene promoter was hypomethylated in preterm placenta as compared to term placenta, while the mRNA levels were comparable between the two groups. The observed imbalance between MMP and TIMP expression may have prematurely triggered the signaling cascade leading to preterm birth. © 2017 IUBMB Life, 69(12):985-993, 2017.

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Section: Discussioncontrasting

confidence: 60%

Investigating the expression of MMPs and TIMPs in preterm placenta and role of CpG methylation in regulating MMP‐9 expression

Sundrani

Narang

Mehendale³

et al. 2017

IUBMB Life

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“…MMP9 has been associated with a prediction of PE but with discordant results. For example, trophoblasts and placentas from pre‐eclamptic pregnancies were recently found to express lower levels of MMP9 (Kolben et al , 1996; Campbell et al , 2004; Montagnana et al , 2009), but some investigators have found higher expression levels of MMP9 in placental sections from PE tissue (Wang et al , 2009). Again, Poon et al (2009), in disagreement with our results, demonstrate that the maternal serum concentration of MMP9 at 11 ± to 13+ weeks of gestation is increased in pregnancies that subsequently develop PE, suggesting an association with an underlying inflammatory process.…”

Section: Discussionmentioning

confidence: 99%

Gene expression in chorionic villous samples at 11 weeks of gestation in women who develop pre‐eclampsia later in pregnancy: implications for screening

et al. 2011

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“…In humans, a decline in the production of MMP‐2 and MMP‐9 was associated with growth retardation (Merchant et al., ). On the other hand, some authors (Kutzler et al., ) have argued that the canine placenta could serve as a model for study of human pre‐eclampsia, considering the high invasive capacity of its cells, the fundamental role of MMPs for invasiveness and the altered expression of these enzymes during pre‐eclampsia (Wang et al., ). Given the importance of the metalloprotease expression and activation, specific tissue and gestational period, it is necessary to perform further studies to establish the location, MMPs expression and activity of canine placenta throughout pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta

Diessler

Ventureira

Hernández

et al. 2016

Reprod Domestic Animals

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The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regarding the latter, the expression of MMP enzymes has been studied in the cat and the mature canine placenta. The aim of this study was to analyse the expression and activity of MMP-2 and MMP-9 in the early dog placenta. Placentae from 18 to 30 days of pregnancy were collected from four bitches. Two placentae from each bitch were analysed. Placental tissue from one uterine horn was fixed in formaldehyde for immunohistochemistry, while marginal haematoma, labyrinth, non-implantative and implantative endometrium from the contralateral horn were immediately frozen in dry ice for the analysis of MMP expression (Western blot [WB]) and activity (zymography). MMP-2 and MMP-9 were evidenced in the labyrinth, maternal glands and marginal haematoma; this finding was directly correlated with levels of MMP expression by WB, and with the activity of MMP-2, mainly in the haematoma (the area of major remodelling of tissues). Thus, although MMP-9 is well expressed in the early canine placenta, it is not active. Given the important role of MMPs for invasiveness, maternal-foetal angiogenesis and the establishment of a correct foetal nutrition, the results are consistent with the findings in other species in which the MMP-2 activation precedes the MMP-9 one in early placentation.

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Expressional and epigenetic alterations of placental matrix metalloproteinase 9 in preeclampsia

Cited by 10 publications

References 0 publications

Investigating the expression of MMPs and TIMPs in preterm placenta and role of CpG methylation in regulating MMP‐9 expression

Investigating the expression of MMPs and TIMPs in preterm placenta and role of CpG methylation in regulating MMP‐9 expression

Gene expression in chorionic villous samples at 11 weeks of gestation in women who develop pre‐eclampsia later in pregnancy: implications for screening

Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta

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