Expressional and functional comparisons of five clustered odorant binding proteins in the brown marmorated stink bug Halyomorpha halys

Wang, Zehua; Yang, Fan; Sun, Ang; Song, Jun; Shan, Shuang; Zhang, Yongjun; Wang, Shan-Ning

doi:10.1016/j.ijbiomac.2022.03.084

Cited by 12 publications

(11 citation statements)

References 66 publications

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“…Many early studies have shown that antennae-specific OBPs are crucial to detecting sex pheromones and plant volatiles. For example, five OBP proteins of Halyomorpha halys have similar ligand binding specificity; they show strong binding ability to the alarm pheromone (( E )-2-decenal), the gathering pheromone ((2 E ,4 E ,6 Z )-2,4,6-decentrienoate), and plant volatiles (such as 3-hexyl benzoate and β-ionone) . Similarly, AlinOBP4 binds to compounds structurally different from the sex pheromone components, such as 3-hexanone, 3-hexanol, and beta-pinene .…”

Section: Discussionmentioning

confidence: 99%

“…For example, five OBP proteins of Halyomorpha halys have similar ligand binding specificity; they show strong binding ability to the alarm pheromone ((E)-2-decenal), the gathering pheromone ((2E,4E,6Z)-2,4,6-decentrienoate), and plant volatiles (such as 3-hexyl benzoate and β-ionone). 41 Similarly, AlinOBP4 binds to compounds structurally different from the sex pheromone components, such as 3-hexanone, 3hexanol, and beta-pinene. 42 However, with the development of sequencing technology, bioinformatics, and various genomics, more OBPs have been identified.…”

Section: Obp Protein Mediates Chemical Sensation In Insectsmentioning

confidence: 99%

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Identifying a New Target for BtOBP8: Discovery of a Small Amino Ketone Molecule Containing Benzothiazole Fragments

Wu,

Wang,

et al. 2023

J. Agric. Food Chem.

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Insects rely on odorant-binding proteins (OBPs) for chemical perception, making OBPs a promising target for studying attractants and repellents of pests, such as Bemisia tabaci. However, no reports have reported using B. tabaci OBPs (BtOBPs) as pesticide screening targets. To fill this gap, we obtained BtOBP8 through prokaryotic expression and purification. Then, we confirmed its identity using western blotting and mass spectrometry. Next, we used the sitting drop and hanging drop methods to screen its crystal conditions. Using microscale thermophoresis and isothermal titration calorimetry, we identified the highest affinity ligand, 3l, from 30 compounds. Furthermore, point mutation techniques identified Val119 as a key amino acid residue in binding 31 to BtOBP8. Finally, we tested the bioactivity of B. tabaci Mediterranean and found that 3l more effectively inhibits the bioactivity of B. tabaci MED than imidacloprid. This study presents a new approach for developing green insecticides specific to B. tabaci MED by targeting OBPs. Conclusively, identifying and targeting specific OBPs can create more targeted and effective pest control strategies without relying on toxic chemicals.

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Section: Discussionmentioning

confidence: 99%

Section: Obp Protein Mediates Chemical Sensation In Insectsmentioning

confidence: 99%

Identifying a New Target for BtOBP8: Discovery of a Small Amino Ketone Molecule Containing Benzothiazole Fragments

Wu,

Wang,

et al. 2023

J. Agric. Food Chem.

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“…Both of their content in females was about two times that in male buds. ( Z )-3-Hexenyl benzoate showed specific binding to odorant-binding proteins of Halyomorpha halys and Plautia stali , which was similar to alarm pheromones of both two pests with the function of repelling [ 60 ]. The higher content of ( Z )-3-hexenylbenzoate in female buds indicated that the compound might help female flowers avoid pests and protect female flowers’ pollination and development.…”

Section: Discussionmentioning

confidence: 99%

The Difference of Volatile Compounds in Female and Male Buds of Trichosanthes anguina L. Based on HS-SPME-GC-MS and Multivariate Statistical Analysis

Song

Liu

et al. 2022

Molecules

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Trichosanthes anguina L. (family Cucurbitaceae) is a monoecious and diclinous plant that can be consumed as a vegetable and has anti-inflammatory and antioxidant effects. The chemical composition and content of volatile compounds in female and male buds of T. anguina were explored by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) technology combined with multivariate statistical analysis. The results showed that the content of the volatile compounds was different between female and male buds. 2,2,6-trimethyl-6-vinyltetrahydro-2H-pyran-3-ol and 2,2,6-trimethyl-6-vinyldihydro-2H-pyran-3(4H)-one were the main volatile compounds in both female and male buds. Based on the multivariate statistical analysis of orthogonal projections to latent structures discriminant analysis (OPLS-DA) and t-test, the content of seven compounds was significantly different between female and male buds. The content of three compounds in male buds was higher than that in female, i.e., (E)-4,8-dimethyl-1,3,7-nonatriene, 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene, and (E)-caryophyllene. Conversely, the content of (Z)-4-hexen-1-ol, (Z)-3-hexenyl benzoate, (Z)-3-hexenyl salicylate, and 2-hexen-1-ol in female buds was higher than that in male buds. This is the first report on the difference in the volatile compounds between female and male buds of T. anguina, which enriches the basic research on the monoecious and diclinous plant and provides a reference for the study of plant sex differentiation.

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“…Most of the research has been on the identification and function of OBPs in adult antennae. 12,18,20,21,23,24 However, studies on the OBPs of larvae are significantly few, especially in G. molesta.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Many more putative OBPs are being identified in various insect species, ,, and their function features are being confirmed on a regular basis. , OBPs have a conserved six cysteine residue pattern in their protein sequences and can detect environmental signals involved in insect host, oviposition site, and mate selections, as well as alarm from natural enemies. ,, As a result, OBPs play critical roles in insect–host, insect–insect, and insect–enemy interactions. Most of the research has been on the identification and function of OBPs in adult antennae. ,,,,, However, studies on the OBPs of larvae are significantly few, especially in G. molesta.…”

Section: Introductionmentioning

confidence: 99%

The Odorant-Binding Protein 1 Mediates the Foraging Behavior of Grapholita molesta Larvae

Tan,

Wei,

et al. 2023

J. Agric. Food Chem.

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Since eggs are laid directly on fruit skin, it is typically believed that food odor has little impact on the foraging of Grapholita molesta larvae. It is crucial to note that larvae that hatch on twigs and leaves could need some sort of identification system when foraging. Here, 22 GmolOBP genes were identified from the G. molesta larval transcriptome via the comparison of conserved domain and homology in the protein level. GmolOBP1 had strong affinities for important pear-fruit volatiles, which caused larvae strong behavioral responses. However, after GmolOBP1 silencing, the larvae lost their attraction to methyl salicylate, α-farnesene, butyl acetate, ethyl butanoate, and ethyl hexanoate, and the effects of larvae seeking various pears were significantly reduced. Consequently, GmolOBP1 was required for the reception of pear volatiles and was involved in mediating how G. molesta larvae foraged. Our research revealed the GmolOBP1 foraging signal recognition mechanism as well as potential molecular targets for field pest management.

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Expressional and functional comparisons of five clustered odorant binding proteins in the brown marmorated stink bug Halyomorpha halys

Cited by 12 publications

References 66 publications

Identifying a New Target for BtOBP8: Discovery of a Small Amino Ketone Molecule Containing Benzothiazole Fragments

Identifying a New Target for BtOBP8: Discovery of a Small Amino Ketone Molecule Containing Benzothiazole Fragments

The Difference of Volatile Compounds in Female and Male Buds of Trichosanthes anguina L. Based on HS-SPME-GC-MS and Multivariate Statistical Analysis

The Odorant-Binding Protein 1 Mediates the Foraging Behavior of Grapholita molesta Larvae

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