Expressions and clinic significance of miRNA-143, miRNA- 34A, miRNA-944, miRNA-101 and miRNA-218 in cervical cancer tissues

Wang, Rui; Zhang, Qingwei; Fu, Xiuhong; Wang, Huifen; Liu, Yuling

doi:10.4314/tjpr.v15i7.6

Cited by 7 publications

(2 citation statements)

References 24 publications

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“…miRNAs are a class of small endogenous non-coding single-stranded RNA molecule that widely exist in organisms, with a length of about 19-25 nucleotides [10] . It mainly combines with the 3 'untranslated region (3' UTR) speci city of target gene mRNA in the complete or incomplete pairing manner, leading to direct degradation of target gene mRNA or inhibition of translation of target protein, so as to promote or inhibit gene expression at the post transcriptional level [11][12][13][14] . Many studies have shown the abnormal expression of miRNA in prostate cancer, gastric cancer, non-small cell lung cancer, breast cancer, pancreatic cancer and other tumors [14][15][16] .This abnormal expression affects tumor cell proliferation, apoptosis, migration and invasion, and is closely related to the clinical staging and prognosis of tumors.…”

Section: Introductionmentioning

confidence: 99%

Research on the mechanism of miR-132-3p in inhibiting the proliferation、invasion and metastasis of colon cancer by targeted regulation of PDGF

Lang

et al. 2023

Preprint

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Objective: To investigate the impacts of miR-132-3p on the proliferation, apoptosis, metastasis and invasion of colon cancer cells by targeted regulation of PDGF, so as to provide a new approach and target for studying the development mechanism of colon cancer and targeting gene therapy of colon cancer. Methods: qRT-PCRexperiment was used to detect the expression of miR-132-3p between colon cancer tissue and adjacent tissue.CCK-8 cloning experiment was carried out to observe the results of overexpressed miR-132-3p HCT-116 cells proliferation; Flow cytometry experiment was conducted to examine the results of apoptosis;Transwell chamber experiment and scratch healing experiment were performed to detect the effect of cell invasion and metastasis; Pearson correlation analysis was adopted to evaluate the correlation between miR-132-3p and PDGF;Luciferase experiment was used to detected the luciferase activity;qRT-PCR and Western blot were carried out to detect the PDGF mRNA and protein respectively;Tumorigenesis experiment was conducted in nude mice to verify the inhibitory effect of miR-132-3p on colon cancer HCT-116 cells. Results: Compared with the pericarcinomatous tissues, the expression of miR-132-3p in colon cancer tissue decreased significantly (P<0.05); Compared with the miR-NC group, the proliferation and invasive ability of HCT-116 cells in the miR-132-3pmimic group decreased significantly (P<0.05); The luciferase activity in the wild-type miR-132-3p-mimic group was lower than that in the miR-132-3pNA group obviously(P<0.05);Compared with miR-132-3pmimic+vector group, the proliferation and invasion ability of cells in miR-132-3pmimic+PDGF group increased apparently (P<0.05);Compared with miR-132-3pNC group, the subcutaneous tumor mass in the nude mice in miR-132-3pmimic group was smaller (P<0.05). Conclusion: PDGF is the action target of miR-132-3p. miR-132-3p can inhibit the proliferation, invasion and metastasis of colon cancer cells by controlling the expression of PDGF so as to promote the apoptosis of colon cancer cells.

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Section: Introductionmentioning

confidence: 99%

Research on the mechanism of miR-132-3p in inhibiting the proliferation、invasion and metastasis of colon cancer by targeted regulation of PDGF

Lang

et al. 2023

Preprint

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“…Human periodontal ligament stem cells (hPDLSCs) from periodontal ligament tissue can form new periodontal supporting tissues and exert effects on the functional regeneration and biological repair of periodontal tissue [2]. MiRNAs are classified of short noncoding RNAs (18-25 nucleotides), which function as gene suppressors to downregulate the stability or translational efficiency of target genes by the 3'-untranslated region (3'-UTR), and can regulate cell proliferation, migration, and differentiation [3,4].…”

Section: Introductionmentioning

confidence: 99%

MiR-452 negatively regulates osteoblast differentiation in periodontal ligament stem cells by targeting the polycomb-group protein, BMI1

Mao¹,

Li²,

Peng³

et al. 2022

Trop. J. Pharm Res

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Purpose: To determine whether miR-452 regulates osteoblast differentiation (OD) in human periodontal ligament stem cells (hPDLSCs) by targeting polycomb-group protein BMI1. Methods: hPDLSCs were stimulated to differentiate upon treatment with mineralization liquid. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were used to measure mRNA and protein expressions, respectively. Alkaline phosphatase (ALP) activity and Alizarin red staining were used to determine the osteogenic differentiation (OD) of hPDLSCs. The bioinformatics software, Targetscan, was used to predict the potential target of miR-452, while luciferase assay, qRT- PCR, and western blot were employed to verify the target gene of miR-452, BMI1. Results: MiR-452 was downregulated during the OD of hPDLSCs, but miR-452 overexpression inhibited the OD of hPDLSCs. BMI1 was identified as a direct target gene of miR-452 during the OD of hPDLSCs, while miR-452 overexpression correlated inversely with BMI1 expression during OD of hPDLSCs. Conclusion: Overexpression of miR-452 suppresses the OD of hPDLSCs by targeting BMI1.This study may provide potential diagnostic and therapeutic basis for OD in hPDLSCs.

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miRNAs that regulate apoptosis in breast cancer and cervical cancer

Beilankouhi,

Maghsoodi,

Sani

et al. 2024

Cell Biochem Biophys

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Expressions and clinic significance of miRNA-143, miRNA- 34A, miRNA-944, miRNA-101 and miRNA-218 in cervical cancer tissues

Cited by 7 publications

References 24 publications

Research on the mechanism of miR-132-3p in inhibiting the proliferation、invasion and metastasis of colon cancer by targeted regulation of PDGF

Research on the mechanism of miR-132-3p in inhibiting the proliferation、invasion and metastasis of colon cancer by targeted regulation of PDGF

MiR-452 negatively regulates osteoblast differentiation in periodontal ligament stem cells by targeting the polycomb-group protein, BMI1

miRNAs that regulate apoptosis in breast cancer and cervical cancer

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