Expressions of heat shock and metallothionein genes in the heart of common carp (<i>Cyprinus carpio</i>): Effects of temperature shock and heavy metal exposure

Ali, K. Said; Ferencz, Ágnes; Nemcsók, J.; Hermesz, Edit

doi:10.1556/abiol.61.2010.1.2

Cited by 25 publications

(6 citation statements)

References 22 publications

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“…Further, none of the applied stresses was able to affect the transcription level of hsp90 . Similar results were previously observed in various fish species exposed either to heat or copper 20 53 54 . In zebrafish, several studies also reported that heat shock and/or copper exposure resulted in increased expression of MTs and/or HSPs in different adult tissues 55 56 or in embryos 36 57 .…”

Section: Discussionsupporting

confidence: 91%

DNA methyltransferases and stress-related genes expression in zebrafish larvae after exposure to heat and copper during reprogramming of DNA methylation

Dorts

Falisse

Schoofs

et al. 2016

Sci Rep

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DNA methylation, a well-studied epigenetic mark, is important for gene regulation in adulthood and for development. Using genetic and epigenetic approaches, the present study aimed at evaluating the effects of heat stress and copper exposure during zebrafish early embryogenesis when patterns of DNA methylation are being established, a process called reprogramming. Embryos were exposed to 325 μg Cu/L from fertilization (<1 h post fertilization - hpf) to 4 hpf at either 26.5 °C or 34 °C, followed by incubation in clean water at 26.5 °C till 96 hpf. Significant increased mortality rates and delayed hatching were observed following exposure to combined high temperature and Cu. Secondly, both stressors, alone or in combination, significantly upregulated the expression of de novo DNA methyltransferase genes (dnmt3) along with no differences in global cytosine methylation level. Finally, Cu exposure significantly increased the expression of metallothionein (mt2) and heat shock protein (hsp70), the latter being also increased following exposure to high temperature. These results highlighted the sensitivity of early embryogenesis and more precisely of the reprogramming period to environmental challenges, in a realistic situation of combined stressors.

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Section: Discussionsupporting

confidence: 91%

DNA methyltransferases and stress-related genes expression in zebrafish larvae after exposure to heat and copper during reprogramming of DNA methylation

Dorts

Falisse

Schoofs

et al. 2016

Sci Rep

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“…Heat shock proteins are reported to protect the cells against the damage promoted by oxidative stress [ 47 – 50 ]. These proteins assist in the correct folding of nascent and misfolded proteins.…”

Section: Discussionmentioning

confidence: 99%

“…These proteins assist in the correct folding of nascent and misfolded proteins. HSP genes are known as “stress genes,” serving as indicators of the cellular toxicity of different environmental stressors [ 47 – 50 ]. In this study, larger relative steady-state levels of the specific mRNA of Nrf2, catalase, HSP70, and 83 were observed at the concentration of 5 mg/mL, suggesting an adaptative response of flies to the presence of the lower concentration of MZ in the diet for 15 days [ 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Exposure of Drosophila melanogaster to Mancozeb Induces Oxidative Damage and Modulates Nrf2 and HSP70/83

Saraiva

Ávila

Silva

et al. 2018

Oxidative Medicine and Cellular Longevity

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Mancozeb (MZ), a manganese- and zinc-containing ethylene-bis-dithiocarbamate, is a broad-spectrum fungicide. Harmful effects of this fungicide have been reported in nontarget organisms via a not fully understood mechanism. Drosophila melanogaster has provided remarkable contributions for toxicological studies. This work was aimed at evaluating the biochemical targets and implication of oxidative stress in MZ-mediated toxicity in drosophilas. Exposure of flies for fifteen days to MZ at 5 and 10 mg/mL through the diet impaired locomotor performance and induced fly mortality. In parallel, it caused lipid peroxidation and reactive oxygen species (ROS) formation and Mn overload. MZ inhibited superoxide dismutase and inducted catalase and glutathione S-transferase activities. Nitric oxide and reduced glutathione levels were significantly decreased by MZ. Heat shock proteins (HSP70 and HSP83) and Nrf2 mRNA levels were significantly augmented in MZ-exposed flies. Our study reinforced the use of Drosophila melanogaster as a reliable model for the study of biochemical targets of pesticides, and based on our data, MZ induced oxidative damage and Mn accumulation in a concentration-dependent manner. An adaptative cellular state was inducted by the lower concentration of pesticide, possibly contributing to the slighter damage observed.

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“…Many papers describe its toxic effect on DNA and the damage to and inhibition of relative repair mechanisms (Emmanouil et al, 2007;Joseph, 2009;Pruski and Dixon, 2002), and the general reduction of protein synthesis coupled with an increase in stress proteins (Qian et al, 2012;Said Ali et al, 2010). Cadmium, whose effects in sublethal concentrations on fish metabolism are well known, has been shown to alter the structure and to cause morpho-pathological changes of varying severity in various organs (Atif et al, 2005;Thophon et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c tThe immunotoxic effects of heavy metals on blood leukocytes of sea bass (Dicentrarchus labrax) were examined. The cells, separated by a discontinuous Percoll-gradients, were exposed in vitro to various sublethal concentrations of cadmium and copper (10 À 7 M, 10 À 5 M, and 10 À 3 M) and their immunotoxic effect was then evaluated by measuring neutral red uptake, MTT assay, DNA fragmentation and Hsp70 gene expression. First of all, we demonstrated that the cells treated in vitro could incorporate Cd and Cu. A relationship between heavy metal exposure and dose-time-dependent alterations in responses of leukocytes from blood was found for both metals, but copper was more immunotoxic than cadmium in all assays performed. A significant reduction in the cells' ability to uptake neutral red and viability by MTT assay was recorded, indicating that both cadmium and copper could change the membrane permeability, inducing cellular apoptosis when the concentration of metals reached 10 À 3 M. The apoptotic effect may also explain the high level of cytotoxicity found when the leukocytes were exposed to higher concentration of metals. These results demonstrated that toxic effect of copper and cadmium affect on the mechanisms of cellmediated immunity reducing the immune defences of the organism.

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Expressions of heat shock and metallothionein genes in the heart of common carp (Cyprinus carpio): Effects of temperature shock and heavy metal exposure

Cited by 25 publications

References 22 publications

DNA methyltransferases and stress-related genes expression in zebrafish larvae after exposure to heat and copper during reprogramming of DNA methylation

DNA methyltransferases and stress-related genes expression in zebrafish larvae after exposure to heat and copper during reprogramming of DNA methylation

Exposure of Drosophila melanogaster to Mancozeb Induces Oxidative Damage and Modulates Nrf2 and HSP70/83

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

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