Expressions of human telomerase mRNA component (hTERC) and telomerase reverse transcriptase (hTERT) mRNA in effusion cytology

Abstract

Telomerase is a ribonucleoprotein enzyme that synthesizes telomeric DNA onto chromosomal ends. Its activity has been reported to be a novel diagnostic marker of malignant potential. However, no study has investigated the diagnostic importance of the expressions of human telomerase mRNA component (hTERC) and telomerase reverse transcriptase (hTERT) mRNA in effusion cytology. We investigated the expressions of hTERC and hTERT using in situ hybridization in 20 malignant cytologic specimens from 20 patients with adenocarcinomas disseminating into the pleura or peritoneum, and 16 benign cytologic specimens in pleural or peritoneal fluid (including reactive mesothelial cells) from 16 patients with non-neoplastic diseases. Expressions of hTERC and hTERT mRNA were detected in 95% and 90% of the 20 malignant cytology specimens, respectively, and were apparent within the cytoplasm of malignant cells. However, no expression was detected in reactive mesothelial cells in the 16 benign cytologic specimens. The expressions of hTERC and hTERT mRNA would appear to be a useful addition to cytology examination for diagnosis based on effusion cytology.

In vivo Lipopolysaccharide Tolerance Recruits CD11b⁺ Macrophages to the Liver with Enhanced Bactericidal Activity and Low Tumor Necrosis Factor-Releasing Capability, Resulting in Drastic Resistance to Lethal Septicemia

“…The removed livers were stained with hematoxylin-eosin (HE) for regular histological examination. Also, in situ hybridization of TNF mRNA was performed as described previously [16] . Detailed information is described in the online supplement.…”

Limitations of detection of malignancy in pleural effusions using ELISA‐based TRAP assay: comparison with cytological examination

“…The diagnostic value of the ELISA‐based TRAP assay may be undermined in tuberculosis endemic areas, and telomerase activity should be interpreted particularly cautiously wherever the annual risk of tuberculous infection is still high, for example, in Taiwan 29 . Recently, the in situ TRAP assay and in situ hybridization for human telomerase mRNA component and telomerase reverse transcriptase mRNA have been described 30,31 . These advanced techniques may be helpful for distinguishing between inflammatory and malignant cells in a non‐malignant pleural effusion with detectable telomerase activity.…”

Resveratrol increases CD68+ Kupffer cells colocalized with adipose differentiation‐related protein and ameliorates high‐fat‐diet‐induced fatty liver in mice

“…The sense probe was used as a negative control. To confirm the presence of intact RNA, replicate slides from each sample used for TNF ‐ alpha expression were also evaluated for the expression of housekeeping genes using biotinylated RNA‐positive control oligonucleotides as described previously . The hybridization was performed overnight at 37°C.…”