Expressions of P53 and CD68 in mouse liver withSchistosoma mansoniinfection and the protective role of silymarin

Abstract

Schistosomiasis is one of the major human parasitic diseases in many developing countries and is one of the causes of morbidity and mortality in the human population. The present work has been planned to study the histopathological and immunohistochemical expression of P53 and CD68 in mouse liver tissues experimentally infected with Schistosoma mansoni, in addition to the ameliorating role of silymarin. A total of 50 adult male mice were divided into 5 groups (10 animals each). Groups 1 and 2 were the control and silymarin groups, respectively, while group 3 was the infected group in which the mice were infected with S. mansoni live cercariae for 6 weeks. Groups 4 and 5 were the cotreated and posttreated groups, respectively, in which mice were infected with cercariae of S. mansoni and treated with silymarin during and after Schistosoma infection, respectively. The major histopathological lesions were variable numbers of perioval granulomas, diffuse infiltration of inflammatory cells, mainly eosinophils and small mononuclear cells, and fibrosis of portal areas and interlobular septa. Treatment with silymarin led to a significant reduction in granuloma area in all treated infected mice compared with nontreated infected mice. Immunohistochemical observations of the liver tissues showed a significant increase in the apoptotic proteins P53 and CD68 after the infection with the cercariae of Schistosoma, compared with the control group. The expression of the cytoplasmic P53 and CD68 was very low in the control liver sections. A significant decrease in the expression of the cytoplasmic P53 and CD68 was observed after silymarin treatment.

Ameliorative effects of vitamin B17 on the kidney against Ehrlich ascites carcinoma induced renal toxicity in mice

“…In the immunohistochemical examination for PCNA and P53 proteins, a strong positive reaction in glomeruli and renal tubules were observed in the kidney sections of the Ehrlich carcinoma group according to Tousson et al and Eldaim et al who detected that Ehrlich tumor causes proliferation and apoptosis in renal tissues and induced strong positive expressions in Ki67, P53, and PCNA proteins in the female mice. Also, our results agree with Aldubayan et al who found that Ehrlich tumor‐induced higher expression of PCNA and KI67 proteins that observed in renal and hepatic tissues for mice.…”

Grape Seed Proanthocyanidin Ameliorates Cardiac Toxicity Induced by Boldenone Undecylenate through Inhibition of NADPH Oxidase and Reduction in the Expression of NOX2 and NOX4

“…The present study revealed that intramuscular injection of Boldenone in male rats induces a significant elevation in food intake, fluid intake, RBW, and RHW. Tousson et al [ 13 ] demonstrated that the RBW of male rabbits increased significantly following Boldenone injection. Shabir et al [ 33 ] reported a significant increase in food intake, water intake, and weight gain in male rats after Boldenone injection.…”

“…Rats were divided into four equal groups (10 each) after 2 weeks of acclimatization: normal control (administered vehicle (sesame oil) injection); positive control or GSPE (GSPE was administered via a stomach tube at 50 mg/kg body weight, twice a week) [ 11 ]; Boldenone (treated with Boldenone undecylenate (5 mg/kg/week, i.m.)) [ 13 ]; and Boldenone then posttreatment with GSPE (treated at the doses and routes mentioned above). Boldenone and vehicle were injected in the hind limbs for 8 weeks.…”

Identification of CD68+ neutrophil granulocytes in in vitro model of acute inflammation and inflammatory bowel disease

“…The present findings need to be considered when immunohistological studies on cryostat or paraffin material from human inflammatory disorders are undertaken, as it is conceivable that a potentially erroneous impression of macrophage-derived damage is present [28][29][30].…”